Method for extracting bone calcium and osteocalcin

A technology of osteocalcin and aggregates, which is applied in food extraction, pharmaceutical formulations, functions of food ingredients, etc., can solve the problems of unsatisfactory calcium supplementation and insufficient free calcium content.

Inactive Publication Date: 2018-11-23
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the bone calcium obtained by the above process, although the free calcium content has increased after fermentation, the free calcium content is still not high enough from a superior calcium source, and the effect of calcium supplementation is not ideal.

Method used

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  • Method for extracting bone calcium and osteocalcin
  • Method for extracting bone calcium and osteocalcin
  • Method for extracting bone calcium and osteocalcin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation medium: MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween -80 0.1%, triammonium citrate 0.2%, pH 5.5~6.0, 115℃, sterilize for 30min.

Embodiment 2

[0055] (1) Bone crushing and sterilization;

[0056] (2) Take B. lactis (B. lactis), Lactobacillus bulgaricus (L. bulgaricus) and B. adolescentis (B. adolescentis), the weight ratio of three lactic acid bacteria is 1:2:2; the weight and volume of aggregate and water The ratio is 1:3, the weight ratio of lactic acid bacteria to the aggregate is 0.03%, 5% of the total weight of sucrose is added, and the fermentation is carried out at 40°C for 12 hours, and sterilized at 120°C for 30 minutes;

[0057] (3) Add hydrochloric acid to adjust pH value 1, add pepsin, the weight ratio of aggregate is 0.04%, hydrolysis temperature is 35℃, stir for 1h, add alkali to adjust the pH value to 6, add papain, weight ratio of aggregate is 0.04 %, 40℃, stirring for 1h, heating to kill the enzyme;

[0058] (4) Add 5% of the total weight of glucose, add Leuconostoc cristatum and citric acid, the weight ratio of Leuconostoc cristatum to the aggregate weight is 0.02%, the weight ratio of Leuconostoc lactis ...

Embodiment 3

[0060] (1) Bone crushing and sterilization;

[0061] (2) Take B. lactis (B. lactis), Lactobacillus bulgaricus (L. bulgaricus) and B. adolescentis (B. adolescentis), the weight ratio of three lactic acid bacteria is 1:2:2; the weight and volume of aggregate and water The ratio is 1:5, the weight ratio of lactic acid bacteria to the aggregate is 0.07%, 5% of the total weight of sucrose is added, fermentation is carried out at 50°C for 12 hours, and sterilized at 120°C for 30 minutes;

[0062] (3) Add hydrochloric acid to adjust pH value 3, add pepsin, the weight ratio of aggregate is 0.08%, hydrolysis temperature 40℃, stir for 2h, add alkali to adjust the pH value to 7, add papain, weight ratio of aggregate is 0.08 %, 50℃, stir for 2h, heat to kill the enzyme;

[0063] (4) Add 5% of the total weight of glucose, add Leuconostoc milk fat and citric acid, the weight ratio of Leuconostoc milk fat to aggregate is 0.06%, the weight ratio of Leuconostoc milk fat to citric acid is 1:30, and t...

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Abstract

The invention discloses a method for secondarily fermenting and extracting bone calcium. The method comprises the following steps: smashing bone, fermenting before enzymolysis, performing enzymolysisand fermenting after enzymolysis. Compared with the prior art, the technique disclosed by the invention has the advantage that the free calcium content in the prepared osteocalcin is greatly increased. The finally prepared product tastes nice, the product is fragrant and the odor in the extracts is removed.

Description

Technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first in metabolic bone disease in Western countries. It is self-evident that osteoporosis brings suffering to patients and families. According to statistics, there are currently more than 100 million patients with osteoporosis in my country, and it is expected to increase to 210 million by 2050. Factors causing calcium deficiencies in more and more people: accelerated pace of life and work, heavy work pressure and irregular life, and carelessness in the reasonable combination of dietary structure. Those with daily calcium intake of less than 600 mg can be considered calcium intake Insufficiency, it is easy to cause a lack of calcium in the body; some middle-aged people are stressed at work, lack of self-care awareness, and do not pay attention to outdoor activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/10A23J3/04A23J3/34A23L33/17A61K38/17A61P17/10
CPCA61K38/1709A61P17/10A23J1/10A23J3/04A23J3/341A23L33/17A23V2002/00A23V2400/123A23V2400/513A23V2400/531A23V2200/306A23V2250/542A23V2300/38A23V2300/14
Inventor 黄永亮郁正刚
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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