Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for producing osteocalcin-containing extract

A technology of extract and osteocalcin, which is applied in the field of preparing extracts containing active osteocalcin, can solve the problem of no effective use of bone residues and the like, and achieve the effect of saving cost

Inactive Publication Date: 2010-03-17
TAKARA HOLDINGS
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bone residues have no useful use after extraction; if they were reused, they would be used as fertilizer at best

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for producing osteocalcin-containing extract
  • Process for producing osteocalcin-containing extract
  • Process for producing osteocalcin-containing extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Study on Solvents for Extracting Active Osteocalcin from Porcine Bone(1)

Prepare the extraction solvent as follows. 0.2M carbonate buffer (pH 9.5): 0.275 g of sodium carbonate (NACALAITESQUE, INC.) and 0.62 g of sodium bicarbonate (NACALAITESQUE, INC.) were dissolved in distilled water to make 50 mL. 1M Hydrochloric acid: 45.7 mL of distilled water was mixed with 4.3 mL of hydrochloric acid (NACALAI TESQUE, INC.). 1 M phosphate buffer (pH6.7): 7.1 g disodium hydrogen phosphate (NACALAI TESQUE, INC.) was dissolved in distilled water to make 50 mL; 6.8 g potassium dihydrogen phosphate (NACALAI TESQUE, INC.) was dissolved in distilled water , to make 50 mL, and then mix the two solutions to adjust to pH 6.7. Then, 10 mL of each extraction solvent was added to 3 g (wet weight) of the mixer-milled bone extract extraction residue (cooked bone) of raw pig bone using hot pressurized water, and the mixture was extracted at 4° C. for 24 hours with stirring. After separating the extrac...

Embodiment 2

Study on the Raw Materials for Extracting Active Osteocalcin from Pork Bone

Raw pork bones were heated in how water (90° C.) for 30 minutes to obtain heating extraction solution 1 . Take out the remaining pork bones and grind them roughly. Then, the coarsely ground pork bones were further heated in how water (90° C.) for 30 minutes to obtain hot extraction solution 2 and hot water-treated pork bones. Then, the raw pig bone was heated in hot water under pressure (120° C.) for 5 hours to obtain the heating extraction solution 3 and the pig bone treated with hot water under pressure. Then, according to the same method as in Example 1, the extract was prepared, and the content of active osteocalcin in the extract was determined, except that 20 g (wet weight) was treated with hot water or 20 g (wet weight) was treated with Autoclaved hot water-treated pork bones were used as raw material bones, both of which were used after coarse grinding with a mixer, and 60 mL of 0.5 M carbona...

Embodiment 3

Study on Salt Concentration of Extraction Solvents Used for Extracting Active Osteocalcin from Porcine Bone

Various concentrations of carbonate buffer were prepared as follows. 1M carbonate buffer (pH 9.5): 1.375 g of sodium carbonate and 3.1 g of sodium bicarbonate were dissolved in distilled water to prepare 50 mL. The solution was diluted with distilled water to obtain a 0.05-0.5M carbonate buffer (pH 9.5). Then, according to the same method as in Example 1, the correlation between the concentration of carbonate buffer solution (pH9.5) and the extraction efficiency of active osteocalcin was studied. The results are shown in Table 3. As shown in Table 3, it was proved that although active osteocalcin was extracted at all tested concentrations, the extraction efficiency of active osteocalcin was particularly high in 0.1-1 M carbonate buffer solution (pH 9.5).

[0078]

table 3

[0079]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A process for producing an extract containing activated osteocalcin; an extract which can be obtained by this production process; a food, a drink, a drug, an oral cavity-care composition and a feed containing this extract; and a growth enhancer containing osteocalcin originating in a bone.

Description

technical field [0001] The present invention relates to a method for preparing an extract containing active osteocalcin; an extract obtained by the preparation method; food or beverage products, medicaments, oral care compositions and feedstuffs containing the extract; Calcium growth promoter. Background technique [0002] Active osteocalcin, also known as Gla-containing bone protein, is a characteristic non-collagen protein synthesized by osteoblasts and bone-forming cells, and it exists in the extracellular matrix. Active osteocalcin has been reported to make up 10-20% of bone non-collagen. [0003] The human osteocalcin molecule contains 49 amino acids, in which glutamic acid residues in the molecule are transformed into gamma-carboxyglutamic acid (Gla) residues through vitamin K-dependent post-translational modification. The γ-carboxylated osteocalcin molecule contains 3 Gla residues (at positions 17, 21 and 24). Active vitamin D promotes the biosynthesis of γ-c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A61K38/00A61P1/14A61P19/08
CPCA61K38/39A61K35/32A61P1/02A61P1/14A61P19/00A61P19/08A61P19/10C07K1/14A61K38/16
Inventor 大野木宏足立真一中川一夫岩元宏毅加藤郁之进
Owner TAKARA HOLDINGS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com