Kit for detecting osteocalcin content and testing method thereof

A technology of osteocalcin and kit, applied in the field of biochemistry, can solve the problems of uncomfortable emergency and timely diagnosis of clinical patients, cumbersome steps of radioimmunoassay, expensive reagents, etc., to improve precision, shorten reaction time, and detection range Extended effect

Inactive Publication Date: 2017-01-25
JIANGSU ZECEN BIOTECH CO LTD
View PDF7 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination
Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.
Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting osteocalcin content and testing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0045] The operation procedure of adopting the test kit test sample of the present invention is as follows:

[0046] 1. Sample collection

[0047] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0048] 2. Preparation before experiment

[0049] ① Take a bottle of lotion a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit using a magnetic particle chemiluminiscence method to measure osteocalcin (BGP) content. The kit comprises a calibration article, a quality control article, a resisting reagent, a magnetic particle reagent and a light-emitting substrate, wherein the resisting reagent comprises an antibody marked by fluorescein isothiocyanate and coated by osteocalcin and an antibody marked by alkaline phosphatase and osteocalcin; the magnetic particle reagent is used for connecting magnetic particles with anti-goat FITC. The kit has the advantages that the chemiluminiscence technology is combined with immune magnetic particles, a reaction system close to homogeneous phase is provided, a one-step reaction mode is used, the detection sensitivity and precision of the kit are increased greatly, the detection range of the kit is increased, reaction time is shortened greatly, time from sample feeding to a detecting result is less than 35 minutes, and the detection speed of the kit is evidently higher than that of similar kits; the kit can detect multiple samples at the same time on full-automatic chemiluminescence apparatus, high-throughput and fast measuring of the osteocalcin is achieved, the kit is high in accuracy and high in specificity, and the accuracy and detection efficiency of the kit are increased greatly.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of osteocalcin (BGP) in a human body by using a magnetic particle chemiluminescence method. Background technique [0002] Osteocalcin, also known as bone γ-carboxyglutamic acid protein (BoneG-Gla Protein, BGP), is an acidic protein. This protein is vitamin K-dependent and contains three γ-carboxyglutamic acid side chains. , the γ-carboxyglutamic acid in BGP is very important to its biological characteristics, and the carboxylated BGP can be the same as Ca 2+ Combined, BGP is one of the most abundant non-collagenous bone proteins in bone, and it is ubiquitous in the bones and dentin of all vertebrates. In adult bones, it accounts for about 25% of the total amount of collagenous bone protein. Vitamin K is the synthesis of BGP Indispensable substance, whose synthesis is regulated by 1,25(OH) 2 D. 3 adjustment. The use of vitamin K antagonists...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 杨旻刘振国薛玲姚广城夏振伟
Owner JIANGSU ZECEN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap