Kit for detecting osteocalcin content and testing method thereof

Abstract

The invention discloses a kit using a magnetic particle chemiluminiscence method to measure osteocalcin (BGP) content. The kit comprises a calibration article, a quality control article, a resisting reagent, a magnetic particle reagent and a light-emitting substrate, wherein the resisting reagent comprises an antibody marked by fluorescein isothiocyanate and coated by osteocalcin and an antibody marked by alkaline phosphatase and osteocalcin; the magnetic particle reagent is used for connecting magnetic particles with anti-goat FITC. The kit has the advantages that the chemiluminiscence technology is combined with immune magnetic particles, a reaction system close to homogeneous phase is provided, a one-step reaction mode is used, the detection sensitivity and precision of the kit are increased greatly, the detection range of the kit is increased, reaction time is shortened greatly, time from sample feeding to a detecting result is less than 35 minutes, and the detection speed of the kit is evidently higher than that of similar kits; the kit can detect multiple samples at the same time on full-automatic chemiluminescence apparatus, high-throughput and fast measuring of the osteocalcin is achieved, the kit is high in accuracy and high in specificity, and the accuracy and detection efficiency of the kit are increased greatly.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of osteocalcin (BGP) in a human body by using a magnetic particle chemiluminescence method. Background technique [0002] Osteocalcin, also known as bone γ-carboxyglutamic acid protein (BoneG-Gla Protein, BGP), is an acidic protein. This protein is vitamin K-dependent and contains three γ-carboxyglutamic acid side chains. , the γ-carboxyglutamic acid in BGP is very important to its biological characteristics, and the carboxylated BGP can be the same as Ca 2+ Combined, BGP is one of the most abundant non-collagenous bone proteins in bone, and it is ubiquitous in the bones and dentin of all vertebrates. In adult bones, it accounts for about 25% of the total amount of collagenous bone protein. Vitamin K is the synthesis of BGP Indispensable substance, whose synthesis is regulated by 1,25(OH) 2 D. 3 adjustment. The use of vitamin K antagonists...

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination

Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.

Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

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