Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bone calcium extraction method by double fermentation and bone gla protein

A secondary fermentation, bone calcium technology, applied in application, food preparation, food science and other directions, can solve the problems of poor taste of the final product, difficult to remove the smell of fishy mutton, etc., and achieve the effect of product aroma and good taste

Active Publication Date: 2013-12-25
TIANJIN TIANSHI BIOLOGICAL DEV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the bone calcium prepared by the above process, although the content of free calcium increases significantly after fermentation, the taste of the final product is poor, and the smell of fishy smell is difficult to remove

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation medium: MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween -80 0.1%, triammonium citrate 0.2%, pH 5.5-6.0, sterilized at 115°C for 30 minutes.

Embodiment 2

[0032] Bone crushing and sterilization; take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of the three kinds of lactic acid bacteria is 1:3:2; aggregate and water The weight to volume ratio is 1:5, the weight ratio of lactic acid bacteria to the aggregate is 0.04%, add 5% sucrose of the total weight, ferment at 50°C for 12h, and sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 3, add pepsin, The weight ratio of the aggregate is 0.08%, the hydrolysis temperature is 40°C, stirring for 2 hours, adding alkali to adjust the pH value to 7, adding papain, accounting for 0.08% of the aggregate weight ratio, stirring at 60°C for 2 hours, heating to kill the enzyme; adding the total weight 5% of glucose, Leuconostoc creamis and citric acid were added, the weight ratio of Leuconostoc creamis to the aggregate was 0.02%, the weight ratio of Leuconostoc creamis and citric ...

Embodiment 3

[0034] Bone crushing and sterilization; take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of three kinds of lactic acid bacteria is 1:3:2; aggregate and water The weight to volume ratio is 1:3, the weight ratio of lactic acid bacteria to aggregate is 0.01%, add 5% sucrose of the total weight, ferment at 35°C for 12h, and sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 1, add pepsin, The weight ratio of the aggregate is 0.04%, the hydrolysis temperature is 35°C, stir for 1h, add alkali to adjust the pH value to 6, add papain, the weight ratio of the aggregate is 0.04%, 40°C, stir for 1h, heat to inactivate the enzyme; add the total weight 5% glucose, adding Leuconostoc creamis and citric acid, Leuconostoc creamis accounted for aggregate weight ratio of 0.06%, Leuconostoc creamis and citric acid weight ratio 1:20, fermentation temperature is 39 ℃, fermentation...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bone calcium extraction method by double fermentation. The bone calcium extraction method by double fermentation includes the steps of fermenting before enzymolysis, enzymolysis and fermenting after enzymolysis. Free calcium content of bone gla protein made by the bone calcium extraction method compared with the prior art is increased greatly. The obtained bone gla protein has fine taste and fragrance, and peculiar odors in extract are removed.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/312A23L1/304A23L13/20
Inventor 黄永亮吴益群赵健崔卜东郁正刚
Owner TIANJIN TIANSHI BIOLOGICAL DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com