Method for extracting bone calcium by fermentation, and osteocalcin

An extraction method and bone calcium technology, applied in the field of food processing, can solve the problems of unbalanced nutritional intake, reduced vitamin D, lack of self-health awareness, etc., and achieve the effects of balanced nutrition and increased calcium content in products

Active Publication Date: 2013-01-16
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can ea...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation medium: MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween -80 0.1%, triammonium citrate 0.2%, pH5.5~6.0, 115℃, sterilized for 30min.

Embodiment 2

[0031] Crush the aggregate, take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of the three lactic acid bacteria is 1:3:2; the weight and volume of aggregate and water Ratio 1:5, add 5% sucrose in weight volume concentration, lactic acid bacteria account for 0.04% by weight of the aggregate, ferment at 50°C for 12h, and sterilize at 120°C for 30min; add bromelain and papain to the aggregate fermentation broth in a weight ratio of 1: 2, the compound protease, the compound protease accounted for 0.05% by weight of the aggregate, added citric acid to adjust the pH value to 5.5, and stirred at 40°C for 1 hour; the enzymatic solution was neutralized with alkali, inactivated, and dried.

Embodiment 3

[0033] Crush the aggregate, take Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), the weight ratio of the three lactic acid bacteria is 1:3:2; the weight and volume of aggregate and water Ratio 1:3, add 5% sucrose by weight and volume concentration, lactic acid bacteria account for 0.01% by weight of the aggregate, ferment for 12 hours at 35°C, and sterilize for 30 minutes at 120°C; add bromelain and papain to the aggregate fermentation broth in a weight ratio of 1: 5 compound protease, the compound protease accounted for 0.2% by weight of the aggregate, added citric acid to adjust the pH value to 6.5, and stirred at 60°C for 2 hours; the enzymatic solution was neutralized with alkali, inactivated, and dried.

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PUM

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Abstract

The invention discloses a method for extracting bone calcium by fermentation, and osteocalcin. The method comprises the steps of crushing the bone, fermenting lactic acid bacteria, heating for sterilization, enzymolizing, adding alkali to neutralize an enzymatic hydrolysate, inactivating and drying to obtain the osteocalcin. Compared with the osteocalcin prepared by a convention technology, the osteocalcin prepared by the enzymolysis technology has greatly increased free calcium content. The final prepared product has better balanced nutrition.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/312A23L33/00A23L13/20
Inventor 吴益群郁正刚黄永亮崔卜东赵健
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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