Quantitative detection kit for neuronspecific enolase (NSE) and preparation method and application thereof

Abstract

The invention relates to a quantitative detection kit for NSE and a preparation method and application of the quantitative detection kit. The kit comprises a calibrator, a magnetic separation reagent, an enzyme reactant, a stable reinforcing agent and a chemiluminiscent substrate, wherein the calibrator is obtained by treating NSE antigen through a reducing agent solution and diluting the NSE antigen to a buffer solution containing a nonionic surfactant; the magnetic separation reagent is obtained by immunofixation of a biotinylation antibody and streptavidin magnetic particles; the enzyme reactant comprises a NSE tracing antibody marked by alkaline phosphatase; and the stable reinforcing agent comprises a multicomponent immune compound interfered by an anti-heterophilic antibody. The invention further relates to a preparation method of the kit and a method of applying the kit to quantitatively detect a tumor marker NSE. The kit is reliable in performance, high in flexibility, and wide in linear range, and matched up with an automatic instrument for use. At present, the kit has already obtained a third registration certificate of a diagnostic reagent in SFDA (State Food and Drug Administration).

Description

Technical field [0001] The invention belongs to the field of medical and biological immunoassay reagents, and specifically relates to a neuron-specific enolase quantitative detection kit and a preparation method and application thereof. More specifically, it relates to a chemiluminescence method based on magnetic particle separation. A kit for detecting tumor marker neuron specific enolase (NSE) and a preparation method thereof, and a method for quantitatively detecting tumor marker neuron specific enolase using the kit. Background technique [0002] Neuron-specific enolase (NSE) is one of the enolases involved in the glycolysis pathway and exists in nerve tissues and neuroendocrine tissues. NSE has the highest activity in brain tissue cells, the activity levels of peripheral nerves and neurosecretory tissues are in the middle, and the lowest values ​​are seen in non-neural tissues, serum and spinal fluid. The destruction of NSE-containing cells will release NSE into the serum, ...

AI Technical Summary

Problems solved by technology

This technique has low sensitivity, narrow linearity, poor specificity, and is not conducive to high-throughput automatic detection

In addition, due to the characteristics of the plate-type detection reagent, the relative validity period is relatively short, and the neuron-specific enolase (NSE) antigen is easily inactivated, and the reliability is low in actual use.

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