Quantitative detection kit for neuronspecific enolase (NSE) and preparation method and application thereof

An enolase quantification and detection kit technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of unfavorable high-throughput automatic detection, low sensitivity, and narrow linearity.

Inactive Publication Date: 2013-02-06
BEIJING DIACHA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique has low sensitivity, narrow linearity, poor specificity, and is not conducive to high-throughput automatic detection
In addition, due to the characteristics of the plate-

Method used

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  • Quantitative detection kit for neuronspecific enolase (NSE) and preparation method and application thereof
  • Quantitative detection kit for neuronspecific enolase (NSE) and preparation method and application thereof
  • Quantitative detection kit for neuronspecific enolase (NSE) and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0065] In the present invention, the capture antibody is a monoclonal antibody that can specifically bind to a neuron-specific enolase (NSE) antigen in humans, and the tracer antibody is a monoclonal antibody that can specifically bind to a neuron-specific enolase antigen. Bound monoclonal antibody. In addition to the specific binding of the capture antibody and the tracer antibody to the neuron-specific enolase (NSE) antigen, they can form a "sandwich" structure with the antigen when paired.

[0066] 1. The neuron-specific enolase (NSE) antigen used in this example was purchased from Tianjian Biopharmaceutical (Tianjin) Co., Ltd. The neuron-specific enolase (NSE) capture antibody and tracer used in this example The antibody was purchased from Tianjian Biopharmaceutical (Tianjin) Co., Ltd. The formulations of the various buffers described in this embodiment are as follows:

[0067] 【PBS pH7.2 buffer】

[0068] Reagent

Vendor

Final concentration

1000ml reagent dosage

Sodiu...

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Abstract

The invention relates to a quantitative detection kit for NSE and a preparation method and application of the quantitative detection kit. The kit comprises a calibrator, a magnetic separation reagent, an enzyme reactant, a stable reinforcing agent and a chemiluminiscent substrate, wherein the calibrator is obtained by treating NSE antigen through a reducing agent solution and diluting the NSE antigen to a buffer solution containing a nonionic surfactant; the magnetic separation reagent is obtained by immunofixation of a biotinylation antibody and streptavidin magnetic particles; the enzyme reactant comprises a NSE tracing antibody marked by alkaline phosphatase; and the stable reinforcing agent comprises a multicomponent immune compound interfered by an anti-heterophilic antibody. The invention further relates to a preparation method of the kit and a method of applying the kit to quantitatively detect a tumor marker NSE. The kit is reliable in performance, high in flexibility, and wide in linear range, and matched up with an automatic instrument for use. At present, the kit has already obtained a third registration certificate of a diagnostic reagent in SFDA (State Food and Drug Administration).

Description

Technical field [0001] The invention belongs to the field of medical and biological immunoassay reagents, and specifically relates to a neuron-specific enolase quantitative detection kit and a preparation method and application thereof. More specifically, it relates to a chemiluminescence method based on magnetic particle separation. A kit for detecting tumor marker neuron specific enolase (NSE) and a preparation method thereof, and a method for quantitatively detecting tumor marker neuron specific enolase using the kit. Background technique [0002] Neuron-specific enolase (NSE) is one of the enolases involved in the glycolysis pathway and exists in nerve tissues and neuroendocrine tissues. NSE has the highest activity in brain tissue cells, the activity levels of peripheral nerves and neurosecretory tissues are in the middle, and the lowest values ​​are seen in non-neural tissues, serum and spinal fluid. The destruction of NSE-containing cells will release NSE into the serum, ...

Claims

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Application Information

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IPC IPC(8): G01N33/573
Inventor 刘劲吕纳新周昊张小平陈峰赵鑫宋旭红仲维新
Owner BEIJING DIACHA BIO ENG
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