VEGF-165 activator and use thereof for promoting stem cell differentiation

A technology of activator and mesenchymal stem cells, applied in the field of stem cells, can solve problems such as infection immune rejection

Active Publication Date: 2018-11-30
ASIA REGENERATIVE MEDICINE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the

Method used

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  • VEGF-165 activator and use thereof for promoting stem cell differentiation
  • VEGF-165 activator and use thereof for promoting stem cell differentiation
  • VEGF-165 activator and use thereof for promoting stem cell differentiation

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Experimental program
Comparison scheme
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Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

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Abstract

The present invention relates to a VEGF-165 activator and use thereof for promoting stem cell differentiation. Alkaline phosphatase (ALP) is an early osteogenesis marker, and is mainly distributed incell membranes to promote cell calcification. The quantitative detection of the ALP can reflect the differentiation level of osteoblasts. If the activity of the ALP is higher, the higher activity of the ALP indicates that pre-osteoblasts have more obvious differentiation tendency to mature osteoblasts. The high expression of the activity of the ALP is an early marker of osteoblast differentiationand maturation. When the activity of the ALP is enhanced, bone formation is enhanced, and bone matrix mineralization is promoted. Therefore, the activity of the ALP is a good indicator for reflectingthe degree differentiation and the functional status of the osteoblasts. The osteogenic differentiation of hBMSCs can be promoted by activating BMP-2 protein expression in the hBMSCs by to-be-tested compounds 1 to 3. The osteogenic differentiation of the hBMSCs can be promoted by activating VEGF-165 protein expression in the hBMSCs by to-be-tested compounds 4 and 5.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/165C12N2506/1353
Inventor 卢珊珊赵元英
Owner ASIA REGENERATIVE MEDICINE LTD
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