24 results about "Cells transplant" patented technology

The invention relates to the technical field of nerve injury repair, in particular to a spinal cord injury repair system based on ordered collagen scaffold carried cell transplantation combined with electroacupuncture stimulation application. The invention provides a spinal cord injury repairing system, which comprises a biological material support transplanting module, a cell inoculating module and a spinal cord injury repairing module, and the electro-acupuncture stimulation module is used for applying electro-acupuncture stimulation to acupuncture points after the biological material stent inoculated with the cells is transplanted to the spinal cord injury part. According to the system, electroacupuncture stimulation and biomaterial stent transplantation cells are combined for application, the microenvironment can be remarkably improved, survival, differentiation and function integration of the transplantation cells can be promoted, meanwhile, muscle atrophy and joint degeneration are relieved, an innovative treatment system is provided for spinal cord injury, and the system has high clinical feasibility and is worthy of popularization and application. Good application prospects are realized in the field of spinal cord injury repair.

Novel methods are disclosed for treating patients with Parkinson's disease and other primary and secondary parkinsonian disorders by improving cell engraftment. Treating patients with antihyperlipidemic drugs and / or CSF-1R antagonists before, during, and / or after transplantation of DA neurons improves cell viability, engraftment, proliferation, migration, or differentiation of the administered DA neurons. Methods are disclosed for pre-treating patients to improve engraftment of administered cells. Methods are disclosed for pre-treating patients to improve engraftment of administered progenitor cells.

The present invention addresses the problem of providing a method for obtaining mature cardiomyocytes which can be used for screening in drug development and for cell transplantation, and said problem is solved by providing a mature cardiomyocyte production method including a step for performing partial reprogramming in immature cardiomyocytes.

Nanoparticles, methods, and kits are provided for supplying nutrients and other therapeutic agents to transplanted cells. Nutrient deprivation is a significant factor which contributes to poor outcome of many cell transplants because cells receive insufficient nutrients until they are able to establish a functional microcirculation to support their metabolic and physiological needs after transplantation. Nanoparticles are provided for use in supplying nutrients and other therapeutic agents to transplanted cells to improve cell survival. Such nanoparticles can be used to supply nutrients and other factors to transplanted cells until the transplanted cells are able to develop a new microcirculation.

The application discloses a kind of fibroblast growth factor 2 and mesenchymal cell co-loaded freeze-dried collagen support and its preparation method and application, belong to the technical field of biomedical materials, by adding sulfated polysaccharide to collagen solution, it is formed freeze-dried collagen support containing sulfated polysaccharide by vacuum freeze-drying, it is combined with fibroblast growth factor 2 incubation, obtain the freeze-dried collagen support of loading fibroblast growth factor 2, again mesenchymal cell is inoculated in the freeze-dried collagen support of loading fibroblast growth factor 2, and the freeze-dried collagen support of fibroblast growth factor 2 and mesenchymal cell co-loading is prepared.The support obtained by the application can efficiently combine fibroblast growth factor 2, support the adhesion growth of mesenchymal cell simultaneously, promote mesenchymal cell to migrate to the inside of support, realize the uniform distribution of cell in support, and can promote angiogenesis, provide new and efficient carrier for cell transplantation treatment.

The invention belongs to the technical field of biological medicine, and particularly relates to composite hydrogel for promoting hiPSC-CMs in-situ maturation and a preparation method and application thereof. According to the preparation method disclosed by the invention, a compound liposome modified by quaternary ammonium chitosan and loaded with Torrin 1 is constructed, a unique three-dimensional network structure (PDN) is constructed by compounding different chitosan, and the compound liposome and the PDN are combined to obtain the composite hydrogel for embedding and delivering hiPSC-CMs. Wherein residual amine of the quaternary ammonium chitosan can slowly release drugs in an acidic microenvironment after myocardial infarction, and the cell cycle is inhibited; the PDN structure enhances the retention rate of cell transplantation; the loaded hiPSC-CMs can restore the contraction function, reduce spontaneous depolarization and action potential, reduce the risk of lethal arrhythmia and promote in-situ maturation of transplanted cells. Experiments show that the gel loaded with the hiPSC-CMs can significantly promote myocardial tissue maturation after myocardial infarction, effectively slow down ventricular remodeling and improve heart functions.

The invention features compositions and methods for treating transplant recipients (e.g., chronic allograft rejection) using a senolytic agent and an angiotensin II receptor antagonist or using a senolytic agent and senomorphic agent. The methods and compositions are useful in a variety of transplant settings including, without limitation, solid organ transplants including kidney, lung, heart, liver, intestine, or pancreas transplantation procedures and cellular transplants including but not limited to bone marrow transplants.

Disclosed is a composition for supporting the survival and differentiation of neural progenitor cells (NPCs) transplanted into a site of nerve injury or disease, the composition comprising (a) a gel-forming molecule and (b) a chemokine receptor type 5 (CCR5) antagonist. Also disclosed is a method for treating nerve injury or disease in a subject, the method comprising: (a) mixing NPCs with a composition disclosed herein; and (b) administering the mixture of NPCs and the composition into the site of nerve injury or disease in the subject, thereby supporting the survival and differentiation of NPCs. Also disclosed is the use of a mixture of NPCs and a composition disclosed herein in the manufacture of a medicament for treating nerve injury or disease in a subject, the mixture being administered into the site of nerve injury or disease in the subject, thereby supporting the survival and differentiation of NPCs. Furthermore, disclosed is a kit for use in supporting the survival and differentiation of NPCs transplanted into the site of nerve injury or disease, the kit comprising: (a) a composition disclosed herein, (b) artificial cerebrospinal fluid (α-CSF), (c) CaCl2, and (d) thrombin.

The invention discloses autologous fat transplantation equipment for increasing the survival rate of fat cells, and relates to the technical field of fat cell transplantation. Comprising an adipocyte transplantation processing mechanism, the adipocyte transplantation processing mechanism comprises a cell transplantation box, and a control panel is installed on the surface of the cell transplantation box; the device can conveniently extract transplanted and extracted fat cells, is beneficial to efficiently treating the transplanted fat cells, improves the fat cell transplantation and extraction effect, reduces the probability of errors caused by manual extraction of the fat cells by medical staff, can ensure that the fat cells are transplanted and extracted in a closed and sterile environment, and improves the survival rate of the fat cells. The fat cell transplantation and extraction device is simple in structure and convenient to use, the survival rate of fat cell transplantation and extraction is increased, medical articles for fat cell extraction can be stored, medical staff can conveniently operate in the fat cell transplantation and extraction work, the fat cell transplantation efficiency of the medical staff is improved, and convenience is provided for the staff.

Provided herein are methods and systems of transplant cells and growing an ectopic tissue in a lymph node of a subject. In certain embodiments, the methods and systems provided herein enable minimally invasive cell transplantation to treat patients in need thereof. In certain embodiments, the methods and systems provided herein include the use of ultrasound endoscopy.

The present disclosure relates to a catechol group- or pyrogallol group-functionalized biocompatible polymer hydrogel patch having excellent biocompatibility and tissue adhesion, and uses for drug delivery, cell transplantation and tissue regeneration using the same. The biocompatible polymer hydrogel patch functionalized with the catechol group or pyrogallol group of the present disclosure has remarkably excellent mechanical properties and tissue adhesion compared with a solution-based bulk hydrogel. Therefore, it can load cells and a drug in vivo for a long time and also safely and efficiently deliver the cells and the drug to a target site.

The present disclosure relates to a porous scaffold having excellent tissue engineering characteristics and a method for preparing the same. The scaffold according to the present disclosure not only can be prepared by a simple process, but also can exhibit a remarkably excellent cell engraftment rate as well as high tensile strength and biocompatibility so that it can be usefully used as a support composition for human body transplantation of various uses, including artificial ligaments and supports for reinforcing the abdominal wall.

An application of using n-butylidenephthalide (BP) in dopamine precursor cell transplantation treatment, including using n-butylidenephthalide (BP) to improve the treatment efficacy of dopamine precursor cell transplantation treatment and using n-butylidenephthalide (BP) and dopamine precursor cells treated by n-butylidenephthalide (BP) in dopamine precursor cell transplantation treatment. The foregoing application is particularly related to using n-butylidenephthalide (BP) to improve the efficacy of dopamine precursor cell transplantation treatment in treating Parkinson's disease.

To provide the first description of hESC-derived cells transplanted into human patients, wherein results are reported for one patient with each of Stargardt's Macular Dystrophy (SMD) and Dry Age-Related Macular Degeneration (AMD).SOLUTION: Controlled hESC differentiation resulted in near-100% pure RPE populations. Immediately after surgery, hyperpigmentation was visible at a transplant site in both patients, with subsequent evidence that cells had attached and integrated into a native RPE layer. No signs of inflammation or hyperproliferation were observed. HESC-derived RPE cells showed no signs of rejection or tumorigenicity at a time of this report. Visual measurements suggest improvement in both patients.SELECTED DRAWING: Figure 1A-B

Disclosed is a composition for supporting survival and differentiation of neural precursor cells (NPCs) grafted into a neurological injury or disease site, the composition comprising: (a) a gel forming molecule; and (b) a chemokine receptor type 5 (CCR5) antagonist. Also disclosed is a method of treating a neurological injury or disease of a subject, comprising (a) mixing NPCs with the composition as disclosed herein; and (b) administering a mixture of the NPCs and the composition into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Also disclosed is use of a mixture of NPCs and the composition as disclosed herein in the manufacture of a medicament for treating a neurological injury or disease of a subject, wherein the mixture is to be administered into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Further disclosed is a kit for use in supporting survival and differentiation of NPCs grafted into a neurological injury or disease site, the kit comprising: (a) the composition as disclosed herein; (b) artificial cerebral spinal fluid (a-CSF); (c) CaCl2; and (d) thrombin.

Disclosed is a composition for supporting survival and differentiation of neural precursor cells (NPCs) grafted into a neurological injury or disease site, the composition comprising: (a) a gel forming molecule; and (b) a chemokine receptor type 5 (CCR5) antagonist. Also disclosed is a method of treating a neurological injury or disease of a subject, comprising (a) mixing NPCs with the composition as disclosed herein; and (b) administering a mixture of the NPCs and the composition into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Also disclosed is use of a mixture of NPCs and the composition as disclosed herein in the manufacture of a medicament for treating a neurological injury or disease of a subject, wherein the mixture is to be administered into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Further disclosed is a kit for use in supporting survival and differentiation of NPCs grafted into a neurological injury or disease site, the kit comprising: (a) the composition as disclosed herein; (b) artificial cerebral spinal fluid (a-CSF); (c) CaCl2; and (d) thrombin.

The present invention relates to a method for isolating dopaminergic neurons and to a pharmaceutical composition for the treatment of Parkinson's disease containing dopaminergic neurons isolated by said method. The method for isolating dopaminergic neurons comprises a step for isolating dopaminergic neurons that are positive for trophoblastic glycoprotein (TPBG), such that the dopaminergic neurons isolated by this method exhibit greater cellular efficacy upon transplantation and greater transplant safety, thus enabling their effective use in cell transplantation for the treatment of Parkinson's disease.

An object of the present invention is to provide a fibrin gel sheet for cell transplantation that has an unprecedented larger size while cells are uniformly dispersed and embedded, and a method for producing the same. The present invention provides a fibrin gel sheet for cell transplantation, wherein cells are uniformly dispersed and embedded in the fibrin gel sheet, and the fibrin gel sheet has a size of 2.25 cm2 or larger in terms of a surface area of one surface and has a thickness of 1 mm or smaller, and a method for producing the same.

The application relates to the field of biotechnology and relates to immune monitoring and imaging, and particularly discloses a base editing ectopic kidney method and a spatial omics imaging monitoring method. The base editing ectopic kidney method comprises the following steps: extracting and screening cytidine deaminase conditional knockout engineered cells of base editing conditional knockout young mice; the cytidine deaminase conditional knockout engineered cells are implanted into a spontaneous immune disease mouse model characterized by nephritis, and an autoimmune-kidney generation model is established. The young mice of APOBEC3 base editing enzyme CD68 cell conditional knockout mice are used as a functional cell source, the cytidine deaminase is not translated, the young mice are suitable for ectopic kidney generation, and the time cost required by single cell amplification, breeding and screening in a traditional cell transplantation scheme is avoided, and the experimental process is greatly simplified.

The present application belongs to the field of biological medicine research, and specifically provides the use of a transcription factor in one or more of the following: preparation of a retinal disease treatment drug; preparation of a retinal ganglion cell regeneration product; preparation of a product for inducing reprogramming of adult cells into retinal ganglion cells; preparation of a retinal ganglion cell; the transcription factor is selected from any one or more of Brn3B, Sox4, Atoh7, Sox11 and Isl1. The cell material used for the regenerated retinal ganglion cells is the endogenous cells of the self, and compared with the cell transplantation, there is no risk of immune rejection and tumorigenesis; the use of viral expression genes is simple and easy to popularize.

The health-care preparation comprises the surfactant provided by the invention and an active ingredient prepared into a solid, a liquid or an emulsion. The invention provides a preparation of a health care product, such as prescription medicine and non-prescription medicine. Mineral, herbal and / or vitamin supplements; medicaments applied in hospitals, clinics, doctor offices and nightseeing care places; vaccines, tissues, organs and cell grafts and / or substitutes and / or infusion agents; and wound care formulations including topical ointments, lotions, cleaners, wipes, bandages, and dressings. The active may be included in the formulation as a solute, solvent, particle, or oil immiscible component of the formulation. The active may be included in a tablet, capsule, tincture, liquid or emulsion. Healthcare formulations of the present invention include formulations suitable for oral, topical and / or injection administration.

Compositions and methods are provided for transplantation of cells to the body, utilizing a thermosensitive polymer that forms a gel when introduced onto tissue or into a cavity of the tissue, such as the eye.

The application provides a traditional Chinese medicine preparation for treating esophageal cancer and a preparation method thereof, and relates to the technical field of traditional Chinese medicine pharmacy. The traditional Chinese medicine preparation is composed of Wilingxian, Shijianchuan, Kelp, Caenotus, Zhongjie wind, Lotus leaf peduncle, Chutoucao, Shashen, Jiuxiangchong, Shiqiti, Huangyaozi, Jiluo, and Luhongfang. The application verifies the anti-esophageal cancer effect and safety of the traditional Chinese medicine preparation through in-vitro and in-vivo experiments. The in-vitro experiment shows that the traditional Chinese medicine preparation can significantly reduce the TNF-alpha, IL-1beta and IL-8 levels in the supernatant of esophageal cancer cells Eca-109, and plays an anti-tumor role by regulating the inflammatory microenvironment. The in-vivo experiment shows that the traditional Chinese medicine preparation can inhibit the growth of esophageal cancer EC9706 cell transplanted tumors in nude mice, remodel the immune balance of the organism by bidirectional regulation of immune cell subgroups, and enhance the anti-tumor immunity. Meanwhile, the traditional Chinese medicine preparation does not cause liver and kidney damage to the nude mice at an effective dose, and provides a new scheme with safety and effectiveness for the clinical treatment of esophageal cancer.

The present invention relates to a conditioning regimen for the transplant of a cell to a subject, optionally wherein the cell is a hematopoietic stem / progenitor cells (HSPC), a CAR cell or a platelet. The invention also relates to methods for the prevention or treatment of a disease or condition in a subject by administration of a cell transplant, wherein said administration comprises the conditioning regimen of the invention.