Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells

A hair follicle stem cell and vascular endothelial technology, applied in the field of cell culture, can solve the problems of easy aging of vascular endothelial cells, restrictions on direct acquisition and application, and limited sources of materials, so as to reduce the probability of immune rejection and disease transmission, and promote effective healing , source-rich effects

Inactive Publication Date: 2016-06-15
HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
View PDF11 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the source of autologous ECs is limited, and it is easy to cause stenosis and occlusion of the grafted blood vessels after harvesting, and it is easy to aggravate the damage to the patient. Moreover, the vascular endothelial cells that have been isolated and cultured in the primary generation are very easy to age, have a short cell cycle, and have very limited proliferation ability.
These all limit the direct acquisition and application of ECs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0035] 1. Isolation, culture, purification and identification of rat hair follicle stem cells

[0036] 1.1 Isolation of rat hair follicle stem cells

[0037] Newborn 1-week-old SD rats were killed by cervical dislocation, put into a beaker filled with 75% ethanol for disinfection, and then taken out. In an ultra-clean bench, use ophthalmic scissors to shorten the tentacles and cut off the skin of the tentacles, rinse once with 75% ethanol, and then rinse three times with PBS. Then digest with 1% collagenase type IV and 1% Dispase enzyme mixture at 37°C for 90min, and wash twice with PBS. Under a stereomicroscope, use a 1ml syringe needle to desheath the hair follicle, cut off both ends, leaving the bulge, inoculate the bulge of the hair follicle into a pre-coated culture dish, and add 1ml of complete medium.

[0038] The components o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells, comprising: (1), isolating rat hair follicle stem cell; (2), culturing the rat hair follicle stem cells; (3), purifying the rat hair follicle stem cells; (4), inhibiting the induced differentiation of the rat hair follicle stem cells into vascular endothelial cells. In the inhibition method provided herein, vascular endothelial cell growth factor 165 is used for the first time as an inducing factor so that the rat hair follicle stem cells efficiently and directionally differentiate into vascular endothelial cells; the formation and growth of vascular endothelial cells generated by induced differentiation of the hair follicle stem cells can be adjusted, an effective healing of a wound is promoted; it is also possible to provide seed cell sources for solving the problems in vascularization of tissue engineering skin, cell-transplant therapy of ischemic disease and the like.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for inhibiting the induced differentiation of hair follicle stem cells into vascular endothelial cells. Background technique [0002] Stem cells are the first element of regenerative medicine, and in-depth research on their biological characteristics can guide the development of regenerative medicine. Stem cells commonly used in regenerative medicine are divided into embryonic stem cells and adult stem cells. Due to the limited sources of embryonic stem cells and serious ethical concerns Ethically controversial, so adult stem cells seem more suitable for application in regenerative medicine. [0003] Studies have shown that hair follicle stem cells (Hair Follicle Stem Cells, HFSCs) are a type of stem cells that exist in the protuberance of the outer root sheath of hair follicles, and have the characteristics of undifferentiation, self-renewal and strong proliferation in vitro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/071C12N5/074
CPCC12N5/069C12N2501/165C12N2506/092
Inventor 全仁夫杜伟斌郑宣李强曹国平
Owner HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com