Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells

A hair follicle stem cell and vascular endothelial technology, applied in the field of cell culture, can solve the problems of easy aging of vascular endothelial cells, restrictions on direct acquisition and application, and limited sources of materials, so as to reduce the probability of immune rejection and disease transmission, and promote effective healing , source-rich effects

Inactive Publication Date: 2016-06-15
HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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Problems solved by technology

However, the source of autologous ECs is limited, and it is easy to cause stenosis and occlusion of the grafted blood vessels after harvesting, and it is easy to aggravate the damage to the patient. Moreover, the vascular endot

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  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells

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Embodiment Construction

[0034] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0035] 1. Isolation, culture, purification and identification of rat hair follicle stem cells

[0036] 1.1 Isolation of rat hair follicle stem cells

[0037] Newborn 1-week-old SD rats were killed by cervical dislocation, put into a beaker filled with 75% ethanol for disinfection, and then taken out. In an ultra-clean bench, use ophthalmic scissors to shorten the tentacles and cut off the skin of the tentacles, rinse once with 75% ethanol, and then rinse three times with PBS. Then digest with 1% collagenase type IV and 1% Dispase enzyme mixture at 37°C for 90min, and wash twice with PBS. Under a stereomicroscope, use a 1ml syringe needle to desheath the hair follicle, cut off both ends, leaving the bulge, inoculate the bulge of the hair follicle into a pre-coated culture dish, and add 1ml of complete medium.

[0038] The components o...

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Abstract

The invention discloses an inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells, comprising: (1), isolating rat hair follicle stem cell; (2), culturing the rat hair follicle stem cells; (3), purifying the rat hair follicle stem cells; (4), inhibiting the induced differentiation of the rat hair follicle stem cells into vascular endothelial cells. In the inhibition method provided herein, vascular endothelial cell growth factor 165 is used for the first time as an inducing factor so that the rat hair follicle stem cells efficiently and directionally differentiate into vascular endothelial cells; the formation and growth of vascular endothelial cells generated by induced differentiation of the hair follicle stem cells can be adjusted, an effective healing of a wound is promoted; it is also possible to provide seed cell sources for solving the problems in vascularization of tissue engineering skin, cell-transplant therapy of ischemic disease and the like.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for inhibiting the induced differentiation of hair follicle stem cells into vascular endothelial cells. Background technique [0002] Stem cells are the first element of regenerative medicine, and in-depth research on their biological characteristics can guide the development of regenerative medicine. Stem cells commonly used in regenerative medicine are divided into embryonic stem cells and adult stem cells. Due to the limited sources of embryonic stem cells and serious ethical concerns Ethically controversial, so adult stem cells seem more suitable for application in regenerative medicine. [0003] Studies have shown that hair follicle stem cells (Hair Follicle Stem Cells, HFSCs) are a type of stem cells that exist in the protuberance of the outer root sheath of hair follicles, and have the characteristics of undifferentiation, self-renewal and strong proliferation in vitro...

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Application Information

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IPC IPC(8): C12N5/071C12N5/074
CPCC12N5/069C12N2501/165C12N2506/092
Inventor 全仁夫杜伟斌郑宣李强曹国平
Owner HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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