Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of iridoid in preparation of anti-osteoporosis medicines

An iridoid and osteoporosis technology, which is applied in the field of medicine, can solve the problems of not knowing the active substances of Ligustrum lucidum, not deeply researching the active ingredients of Ligustrum lucidum, etc., so as to promote the differentiation of osteocytes and promote the proliferation of cells. , the effect of promoting alkaline phosphatase activity

Inactive Publication Date: 2014-08-20
魏园
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on the anti-osteoporosis activity of Ligustrum lucidum mostly focuses on the crude extract of Ligustrum lucidum, as disclosed in CN200710073273.9 in the new medical application of Ligustrum lucidum and its pharmaceutical preparations, it is recorded that Ligustrum lucidum is used in the preparation of prophylaxis or The application of drugs for the treatment of osteoporosis, but there is no in-depth study of the specific active ingredients in Ligustrum lucidum, and it is not known which active substance in Ligustrum lucidum can prevent or treat osteoporosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of iridoid in preparation of anti-osteoporosis medicines
  • Application of iridoid in preparation of anti-osteoporosis medicines
  • Application of iridoid in preparation of anti-osteoporosis medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Tracking separation of anti-osteoporosis active ingredients

[0040]UMR-106 cells belong to the rat osteosarcoma cell line and have many characteristics of osteoblasts. Many studies have proved that UMR-106 cells are a good cell model, which can be used to study the mechanism of action of various hormones and growth factors regulating osteoporosis at the cellular level. Compounds that can promote the activity of alkaline phosphatase (ALP) and cell proliferation in UMR-106 cells indicate that they can promote the function of osteoblasts. Estradiol was found to promote ALP activity in UMR-106 cells and was therefore used here as a positive control.

[0041] 1.1 In vitro culture of UMR-106 cells

[0042] Osteoblast UMR-106 was cultured in a 4.5×9cm cell culture flask with sterile RPMI-1640 culture solution plus 10% newborn calf serum (containing 100U / mL penicillin, 100μg / mL streptomycin) at 37°C, constant 5%CO 2 in the cell culture incubator. Take the cell c...

Embodiment 2

[0104] Example 2 promotes the effect of UMR-106 cell proliferation

[0105] Using Am-blue cell proliferation and activity detection reagent to detect the proliferation of UMR-106 osteoblasts, and compared with the blank control (only the same amount of solvent DMSO was added), the effects of various extracts and compounds on the proliferation of UMR-106 cells were detected. effect.

[0106] 2.1 Principle of Am-blue cell proliferation assay

[0107] AlamarBlue cell proliferation detection method is a newly developed cell proliferation detection method. Its principle is that during the process of cell proliferation, the environment in the cell body changes from an oxidative environment to a reducing environment. NADPH / NADP, FADH / FAD in the respiratory chain, Ratio of FMNH / FMN and NADH / NAD was elevated. After AlamarBlue is endocytosed by cells, it is reduced by the above metabolic intermediates in the cytoplasm and finally released to the outside of the cell. The accumulation ...

Embodiment 3

[0120] Embodiment 3 removes the ability of DPPH free radical

[0121] Absorbance value A detected after reacting each compound with different concentrations with DPPH free radical i , the absorbance value of the respective control tube A j , the absorbance value of the blank tube A b And standard tube absorbance value A 0 Substituting formula (1) to calculate the scavenging rate of each concentration of each compound to DPPH free radicals, take the scavenging rate as the ordinate, and the concentration of each compound as the abscissa plot to obtain the regression equation, and calculate the half free radical scavenging rate of each compound to DPPH Concentration IC 50 When the concentration reaches 500mg / L, the scavenging rate of DPPH free radicals does not reach 50%, the result is then selected to represent the maximum DPPH free radical scavenging rate when the concentration is 500mg / L, and the results are shown in Table 5. Compounds 1 and 3 have strong ability to scaven...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicine, and relates to the application of an iridoid compound in the preparation of anti-osteoporosis drugs. The application of the iridoid compound of general formula I of the present invention in the preparation of medicines for treating and / or preventing osteoporosis. The experiment found that the extract of iridoids with the general formula I was tracked and separated based on the ALP activity in UMR106 cells, and the effects of the extract and the separated components on osteoblast proliferation and antioxidant activity Research has been carried out, showing that the iridoid compounds with the general formula I can significantly promote the activity of alkaline phosphatase and promote the differentiation of osteoblasts, so this type of compound has an anti-osteoporosis effect and can be used to prepare Medicines to treat or prevent osteoporosis. Among them, privetin and compound G13 have the effect of significantly promoting the activity of alkaline phosphatase and promoting the differentiation of osteoblasts, and the effect of privetin can also significantly promote the proliferation of UMR-106 cells, which is used for anti-osteoporosis When the effect is better.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of iridoid compounds in the preparation of anti-osteoporosis drugs. Background technique [0002] Osteoporosis is a disease characterized by damage to the microstructure of bone tissue, a decrease in the proportion of bone mineral composition and bone matrix, bone thinning, a decrease in the number of bone trabeculae, increased bone fragility, and increased risk of fracture. Diseases of systemic bone metabolism disorders. Osteoporosis has become an important issue affecting the health of our people and social medical security, and has become the second leading cause of death in the elderly, second only to cardiovascular and cerebrovascular diseases. According to the survey, it is estimated that in 2006, about 69.44 million people (15.34 million males and 54.1 million females) suffered from osteoporosis, and about 213.9 million people had low bone mas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P19/10G01N30/90
Inventor 王飞陈前锋杨莉娟张国林魏园
Owner 魏园
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com