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Imidacloprid fluorescence immunoassay method based on gold nano-cluster anchored hydroxy cobalt oxide nanosheet

A fluorescent immunoassay and cobalt oxyhydroxide technology, applied in the field of biosensors, can solve the problems of cumbersome test methods, large errors, low detection sensitivity, etc., achieve great practical application value, excellent enzymatic reaction, and improve the effect of detection sensitivity

Active Publication Date: 2019-04-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of low detection sensitivity, large error and cumbersome test methods in the existing detection technology, and provide a gold nanocluster (AuNCs)-anchor based The composite material of cobalt oxyhydroxide (CoOOH) nanosheets and its preparation method are used in the imidacloprid fluorescence immunoassay method to realize the simple and high-sensitivity detection of imidacloprid in complex matrices, thereby promoting the practicality of such sensors in pesticide detection change

Method used

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  • Imidacloprid fluorescence immunoassay method based on gold nano-cluster anchored hydroxy cobalt oxide nanosheet
  • Imidacloprid fluorescence immunoassay method based on gold nano-cluster anchored hydroxy cobalt oxide nanosheet
  • Imidacloprid fluorescence immunoassay method based on gold nano-cluster anchored hydroxy cobalt oxide nanosheet

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Synthesis of CoOOH NPs

[0025] First prepare CoOOH nanosheets, and 125μL KOH (1.0mol L -1 ) And 500μL CoCl2 (10mol L -1 ) Mixed, then sonicated for 1 min; 25μL NaClO (0.9mol L -1 ) Add to the above solution, continue to sonicate for 10 minutes, and then centrifuge at 10000 rpm for 10 minutes to collect the CoOOH NPs product. Washed with deionized water three times, and freeze-dried the obtained CoOOH NPs black powder. Finally, dilute CoOOH NPs with deionized water to 0.025mg mL -1 spare.

Embodiment 2

[0026] Example 2: Preparation of AuNCs functionalized with glutathione

[0027] Add 0.60 mL of HuCl 4 (20mmol L -1 ) And 0.15mL of GSH (100mmol L -1 ) Mix, add 4.55 mL of ultrapure water under vigorous stirring. Then, the above mixture was heated to 70°C for 24 hours to obtain a yellow AuNCs solution, purified by dialysis (1kDa), and freeze-dried to obtain yellow AuNCs powder. Finally, the AuNCs powder was diluted with deionized water to 0.20 mg mL -1 spare.

Embodiment 3

[0028] Example 3: Preparation of CoOOH-AuNCs composite material

[0029] Take the 5.0mL CoOOH NPs solution (0.25mg mL -1 ), added to the AuNCs solution of Example 2 (5.0mL, 0.20mg mL -1 ), the mixture was sonicated for 10 minutes, and then the mixed solution was centrifuged at 10000 rpm for 10 minutes, and the precipitate was dissolved in 10 mL of deionized water. The CoOHO-AuNCs composite material solution was obtained.

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Abstract

The invention discloses an imidacloprid fluorescence immunoassay method based on gold nano-cluster anchored hydroxy cobalt oxide nanosheet, and belongs to the technical field of the biological sensor.A nano composite material is formed by anchoring the gold nano-cluster on a surface of the two-dimensional hydroxyl cobalt oxide nanosheet, and the fluorescence intensity is obviously reduced. The ascorbic acid capable of triggering CoOOH nanosheet decomposition is imported to effectively reverse a quenching effect. Noticeable, the corresponding fluorescence reaction induced by the ascorbic acidis related to the ALP activity of the antibody mark. After the competitive immunoreaction, the antibody of the ALP mark can be combined with the immobilized antigen, the fluorescence change of a detection platform can be adjusted. By utilizing the fluorescence switching of the system, the detection concentration (IC50) on the imidacloprid by the FIA is 1.3ng mL-1, and is 60 times sensitive than the the conventional ELISA (86.4ng mL-1). The fluorescence immunoassay method can realize the high-sensitive detection of the target antigen imidacloprid, a new prospect is opened for the pesticide detection, and an effective policy is opened for the fluorescence immunoassay.

Description

Technical field [0001] The invention belongs to the technical field of biosensors, and specifically relates to a method for preparing a composite material based on gold nanoclusters anchoring cobalt oxyhydroxide nanosheets (CoOOH NPs), and cleverly establishes alkaline phosphatase (ALP)-mediated fluorescence immunoassay for imidacloprid Analytical method. Background technique [0002] Nowadays, pesticide pollution has become a worldwide public health problem. Its outbreak not only causes huge economic losses, but also seriously endangers human life. Imidacloprid, as a contact nicotinic pesticide, acts on the post-synaptic nicotinic acetylcholine receptors to block the normal conduction of the central nervous system and paralyze the pests and die. Similarly, imidacloprid residues in agricultural products and environmental samples are also harmful to the neuroreceptors of animals and humans. It may even damage the DNA of human peripheral blood lymphocytes, which can pose a huge ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551G01N33/535G01N21/64
CPCG01N21/643G01N33/535G01N33/551G01N2021/6432
Inventor 卢革宇闫旭李红霞金蕊孙鹏刘方猛
Owner JILIN UNIV
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