3D printed sodium alginate-type I collagen-ceramic composite scaffold, preparation method and application

A sodium alginate and 3D printing technology, which is applied in prosthesis, tissue regeneration, additive processing, etc., can solve problems such as the inability to bear the huge stress of the knee joint, poor mechanical properties of fibrocartilage, and inability to guarantee long-term curative effect, and achieve improved Chondrocyte proliferation and ALP activity, reduce chronic inflammatory response, and facilitate cell adhesion

Active Publication Date: 2020-10-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current clinical treatment methods such as microfracture, osteochondral column transplantation, and chondrocyte transplantation do not involve combined treatment of osteochondral injury, and the new cartilage tissue is often fibrocartilage rather than hyaline cartilage.
Fibrocartilage has poor mechanical properties and cannot withstand the huge stress generated by the daily activities of the knee joint, and is prone to degeneration and wear, so long-term efficacy cannot be guaranteed

Method used

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  • 3D printed sodium alginate-type I collagen-ceramic composite scaffold, preparation method and application
  • 3D printed sodium alginate-type I collagen-ceramic composite scaffold, preparation method and application
  • 3D printed sodium alginate-type I collagen-ceramic composite scaffold, preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0039] 1) The calcium magnesium silicate powder is processed by wet ball milling to obtain an ultrafine powder with a particle size of no more than 5 μm, and the ultrafine powder is dispersed in deionized water, stirred at room temperature for 1 hour, and then added with I The type collagen powder was stirred at room temperature for 5 minutes, and finally sodium alginate was added and stirred at room temperature for 15 minutes to make a hydrogel as a composite material ink;

[0040] Among them, the mass fraction ratio of sodium alginate: type I collagen: calcium magnesium silicate is 6:2:5.

[0041] The calcium-magnesium silicate is magnesium-doped wollastonite, and the molar percentage of magnesium replacing calcium in the magnesium-doped wollastonite is 10%.

[0042] 2) Put the hydrogel into a three-dimensional printer and print it with a three-dimensional printer. The length of the scaffold is 10 mm, the pore size of the channel is 300 μm, and the porosity is 43%. During ...

Embodiment 2

[0045] 1) Treat the β-tricalcium phosphate powder with wet ball milling to obtain an ultrafine powder with a particle size of no more than 5 μm, disperse the ultrafine powder into deionized water, and stir at room temperature for 1 hour, then add I The type collagen powder was stirred at room temperature for 5 minutes, and finally sodium alginate was added and stirred at room temperature for 15 minutes to make a hydrogel as a composite material ink;

[0046] The mass fraction ratio of sodium alginate: type I collagen: β-tricalcium phosphate is 6:2:5.

[0047] 2) Put the hydrogel into a three-dimensional printer and print it with a three-dimensional printer. The length of the scaffold is 10 mm, the pore size of the channel is 300 μm, and the porosity is 43%. During the printing process, 10% calcium chloride spray was used for cross-linking. After printing, the scaffold was soaked in 10% calcium chloride solution for 10 minutes for further cross-linking to obtain the final 3D p...

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Abstract

The invention discloses a 3D printed sodium alginate-collagen type I-ceramic composite scaffold and a preparation method and application thereof. The composite scaffold is mainly composed of sodium alginate, collagen type I and calcium magnesium silicate according to a mass ratio of (1-6): 2: (0-10) and utilizes calcium chloride as a crosslinking agent, wherein a mass fraction of calcium chloride in the composite scaffold is 1 to 10%. The preparation method comprises dispersing the calcium magnesium silicate powder in deionized water, fully mixing the solution, compounding the solution, collagen type I and sodium alginate to obtain uniform hydrogel, placing the hydrogel in a 3D printer, carrying out crosslinking with calcium chloride and printing a porous scaffold which is the composite scaffold. The composite scaffold can promote chondrocyte proliferation and ALP activity, has excellent biological activity and has an application value in tissue engineering.

Description

technical field [0001] The invention relates to medical biomaterials, in particular to a 3D printed sodium alginate-type I collagen-ceramic composite scaffold, a preparation method and application. [0002] technical background [0003] Cartilage defect is a common disease in orthopedics, especially in athletes, and cartilage damage is usually combined with subchondral bone damage. Patients often have joint swelling, pain, and limited activities, which affect daily life and work, and cause a large economic burden to the society. There are no blood vessels, no nerves, no lymph in the cartilage, poor self-repair ability after injury, and difficult treatment, which has always been a problem in orthopedics. However, the current clinical treatment methods such as microfracture, osteochondral column transplantation, and chondrocyte transplantation do not involve combined treatment of osteochondral injury, and the new cartilage tissue is often fibrocartilage rather than hyaline car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/10A61L27/56A61L27/50B33Y70/10B33Y80/00
CPCA61L27/10A61L27/20A61L27/24A61L27/50A61L27/56A61L2430/06B33Y70/00B33Y80/00C08L5/04
Inventor 余新宁戴雪松苟中入杨贤燕沈炜亮赵腾飞方晶华
Owner ZHEJIANG UNIV
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