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Shark protein antihypertensive peptide and preparation method and application thereof

A technology of antihypertensive peptides and protease preparations, which is applied in the field of marine biology and can solve problems such as staying in the primary stage

Inactive Publication Date: 2008-08-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research and application of sharks is limited to shark liver oil and shark cartilage extracts, but shark liver oil and shark cartilage are only a small part of the shark body, the research and application of most shark materials except shark liver oil and shark cartilage still in its infancy

Method used

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  • Shark protein antihypertensive peptide and preparation method and application thereof
  • Shark protein antihypertensive peptide and preparation method and application thereof
  • Shark protein antihypertensive peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Three peptides Cys-Phe, Glu-Tyr and Phe-Glu were obtained by separation and purification from shark hydrolyzate.

[0041] (1) Preparation of protease preparation

[0042] Seed culture medium: take 0.3 part of beef extract, 1 part of peptone, 2 parts of agar, 0.5 part of NaCl as medium by weight, 98 parts of water, pH is 7.1, sterilized, streaked and connected to Bacillus subtilis SM98011 (Bacillus.SubtilisSM98011) Bacteria were cultured at 30°C for 20 hours.

[0043] Fermentation medium: 3 parts of bean cake powder, 2 parts of corn flour, 1 part of bran, 0.4 part of Na2HPO4, 0.04 part of KH2PO4, 100 parts of water, and 0.3 part of soybean oil as defoamer. Sterilize at 120°C for 30 minutes, after cooling, put 10 parts of the bacterial suspension connected with seeds into a 150-liter fermenter with a sample volume of 70 liters, and at 30°C, the ventilation rate (V 风 / V 液 ·Time) 1:0.6, stirring at 330 rpm, and culturing for 36 hours. At this point the pH was...

Embodiment 2

[0051] Embodiment 2: as described in embodiment 1, difference is that the consumption of raw material shark is not the same, gets the shark meat of 45 parts (fresh weight), beating. Then add 50 parts (liquid weight) of protease preparation.

[0052] The short peptides of the present invention have higher ACE inhibitory activity, because the three peptides of the present invention are dipeptides, therefore, in the digestive tract, it is difficult to be further enzymatically hydrolyzed and can be directly absorbed and utilized. It will exert better antihypertensive activity. The present invention is characterized in that it integrates enzyme engineering technology and peptide purification and identification technology, deeply develops and utilizes the protein part of sharks that has not been effectively used so far, and discovers an ACE inhibitory peptide composed of a new amino acid sequence and guarantees The functionality in the body is confirmed, which lays the foundation f...

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PUM

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Abstract

Shark protein antigypertensive peptide, preparation and application thereof, which pertains to marine biology technical field. The novel antigypertensive peptide with two amino acid separated and purified from proteolysis product of shark, has a high inhibiting activity for angiotensin converting enzyme (ACE). Preparation includes steps of carrying out enzymolysis for shark protein to prepare enzymatic hydrolysate, seperating and purifying peptide from the enzymatic hydrolysate, and sequencing of peptide. The invention is applicable for preparing antihypertensive medicament. The invention apply microbial enzyme technology to deep development of shark, obtain peptide having novel sequence, with high inhibiting activity for ACE.

Description

technical field [0001] The invention relates to an antihypertensive peptide with a new amino acid sequence isolated and purified from an enzymatic hydrolysis product of shark meat, a preparation method and application thereof, and belongs to the technical field of marine biology. Background technique [0002] Short peptides obtained by partial enzymatic hydrolysis of food proteins have attracted more and more attention from food scientists. These short peptides have various activities, such as: enhancing immunity, increasing nutrient absorption rate, antihypertensive activity. Proteins with these activities have been purified from food protein hydrolysates. These peptides are inactive within the protein sequence and are only active when released from the protein by enzymatic hydrolysis or food processing. [0003] Angiotensin-converting enzyme (ACE) is a carboxypeptidase that can catalyze the conversion of angiotensin 1 into angiotensin 2, which can lead to an increase in b...

Claims

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Application Information

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IPC IPC(8): C07K5/065C07K5/072C07K5/06C07K1/20C07K1/34C12P21/06
Inventor 张玉忠吴昊何海伦陈秀兰周百成
Owner SHANDONG UNIV
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