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Filefish skin collagen peptide having ACE restraining activity and preparation method of filefish skin collagen peptide

A technology for inhibiting activity and horse-faced fish, applied in the preparation of horse-faced fish skin collagen peptides, in the field of horse-faced fish skin collagen peptides, to achieve high enzymatic hydrolysis efficiency, avoid environmental pollution, and improve resource utilization

Inactive Publication Date: 2019-07-16
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few reports on the preparation of collagen peptides with ACE-inhibiting activity from horse-faced fish skin

Method used

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  • Filefish skin collagen peptide having ACE restraining activity and preparation method of filefish skin collagen peptide
  • Filefish skin collagen peptide having ACE restraining activity and preparation method of filefish skin collagen peptide
  • Filefish skin collagen peptide having ACE restraining activity and preparation method of filefish skin collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The preparation method of the horse-faced fish skin collagen peptide with ACE inhibitory activity in this embodiment comprises the following steps:

[0065] (1) Pretreatment of horse-faced fish skin

[0066] After the horse-faced fish skin is washed and minced, add the isopropanol with a volume concentration of 5% according to the solid-liquid weight ratio of 1:20, soak and degrease for 10 hours, filter to remove the isopropanol, wash the filter residue with distilled water, and then remove the filter residue Add the NaCl solution with a mass concentration of 10% according to the solid-liquid weight ratio of 1:5, filter after soaking for 12 hours, and wash the obtained filter residue with distilled water for later use;

[0067] (2) Preparation of horse-faced fish skin collagen

[0068] Add the filter residue obtained at the end of step (1) into acetic acid with a concentration of 0.5 mol / L and soak for 12 hours according to the solid-to-liquid weight ratio of 1:10, and...

Embodiment 2

[0074] The preparation method of the horse-faced fish skin collagen peptide with ACE inhibitory activity in this embodiment comprises the following steps:

[0075] (1) Pretreatment of horse-faced fish skin

[0076] After the horse-faced fish skin is washed and minced, it is added into isopropanol with a volume concentration of 15% according to the solid-liquid weight ratio of 1:10, soaked and degreased for 20 hours, then filtered to remove the isopropanol, and the filter residue is washed with distilled water before the filter residue Add the NaCl solution with a mass concentration of 7% according to the solid-liquid weight ratio of 1:10, filter after soaking for 24 hours, and wash the obtained filter residue with distilled water for later use;

[0077] (2) Preparation of horse-faced fish skin collagen

[0078] Add the filter residue obtained at the end of step (1) into acetic acid with a concentration of 0.1mol / L according to the solid-to-liquid weight ratio of 1:30 and soak...

Embodiment 3

[0084] In this embodiment, the preparation method of the horse-faced fish skin collagen peptide with ACE inhibitory activity comprises the following steps:,

[0085] (1) Pretreatment of horse-faced fish skin

[0086] After the horse-faced fish skin is washed and minced, add it to isopropanol with a volume concentration of 10% according to the solid-to-liquid weight ratio of 1:15, soak and degrease for 15 hours, filter to remove the isopropanol, wash the filter residue with distilled water, and then remove the filter residue Add the NaCl solution with a mass concentration of 8% according to the solid-liquid weight ratio of 1:7, filter after soaking for 18 hours, and wash the obtained filter residue with distilled water for later use;

[0087] (2) Preparation of horse-faced fish skin collagen

[0088] Add the filter residue obtained at the end of step (1) into acetic acid with a concentration of 0.3mol / L according to the solid-to-liquid weight ratio of 1:20 and soak for 24h, th...

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Abstract

The invention relates to filefish skin collagen peptide having ACE restraining activity. The amino acid sequence of the collagen peptide is Gly-Val-Gla, and through UPLC-MS detection, the molecular ion peak is [M]+329m / z. The invention further relates to a preparation method of the collagen peptide. The preparation method comprises combined usage of mixing and hydrolysis through double enzymes, ultrafiltration and a gel column chromotography technique, the whole preparation process is simple and easy to control, the enzymolysis efficiency is high, and the obtained collagen peptide is safe andfree from side effects, has higher ACE restraining activity and also has better heat stability; besides, after being digested through digestive tracts of stomach and intestines, the collagen peptide still maintains higher ACE restraining activity; and in addition, processed waste namely fishskin is used as a raw material for preparing the collagen peptide, so that environmental pollution is effectively avoided, and the utilization rate of the resources is increased. The filefish skin collagen peptide has higher practical value and commercial value.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of aquatic product processing, and in particular relates to a horse-faced fish skin collagen peptide with ACE inhibitory activity, and also relates to a preparation method of the horse-faced fish skin collagen peptide. Background technique [0002] At present, hypertension is a common cardiovascular disease, which seriously affects human health. Blood pressure is regulated under the action of angiotensin-converting enzyme (ACE), which catalyzes the conversion of inactive angiotensin I into angiotensin II, which has the activity of contracting vascular smooth muscle and causing blood pressure to rise; on the other hand, ACE can Convert bradykinin, which has vasodilation effect, into slow-release peptide with no vitality. ACE inhibitors play a role in lowering blood pressure by inhibiting the activity of ACE and blocking the two biochemical reaction processes caused by ACE. ACE inhibitors ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/36C07K1/34C07K1/16C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 曹少谦戚向阳马丹妮赵震震王正东杨华
Owner ZHEJIANG WANLI UNIV
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