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Angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and preparation method thereof

An angiotensin and protein-derived technology, applied in the field of angiotensin-converting enzyme (ACE) inhibitory peptides derived from amygdalin and its preparation, achieving high safety effects

Inactive Publication Date: 2013-05-29
SHAANXI UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, food-derived ACE inhibitor peptides are mainly composed of 2-11 amino acids and oligopeptides with a molecular weight of less than 1KDa. Insect proteins, no reports of ACE inhibitor peptides derived from amygdalin

Method used

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  • Angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and preparation method thereof
  • Angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and preparation method thereof
  • Angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and preparation method thereof

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Embodiment Construction

[0027] The invention will be further described below in conjunction with the accompanying drawings.

[0028] (1) Preparation of bitter almond protein isolate

[0029] After manually peeling the bitter almonds, soak them in 0.3% hydrochloric acid for 6 hours, then change the water for 42 hours, change the water every 6 hours to remove the amygdalin in the bitter almonds, and then dry them in an oven at 40°C for 24 hours. Then crush the dried bitter almonds, degrease them with petroleum ether (the mass ratio of bitter almonds and petroleum ether is 1:6) three times, dry them at 40°C to remove the petroleum ether, and pass through a 60-mesh sieve to obtain defatted bitter almond powder , using the alkali-soluble acid precipitation method to extract bitter almond protein. In the alkali-soluble acid precipitation method: in the alkali-soluble stage, add the defatted bitter almond powder to water, adjust the temperature of the system to 50 ° C, adjust the pH value of the system to 1...

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Abstract

The invention provides an angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and a preparation method thereof. The inhibitor is pentapeptide of which the relative molecular mass is 688 and the amino acid sequence is Thr-Tyr-Gly-Trp-Tyr. The preparation method comprises the following steps: debitterizing and detoxifying peeled bitter almond raw material, degreasing with petroleum ether, extracting and separating the protein by an alkali fusion acid precipitation method, carrying out enzymolysis with an alkaline proteinase, carrying out centrifugal separation on the enzymolysis solution, taking the supernatant, separating with an ultrafiltration membrane, and separating and purifying by gel filtration chromatography and reversed phase high performance liquid chromatography to obtain the new ACE (angiotensin converting enzyme) inhibitory peptide.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals or functional food processing, and relates to polypeptide structures and sequences, in particular to an angiotensin-converting enzyme (ACE) inhibitory peptide derived from amygdalin and a preparation method thereof. Background technique [0002] Many natural protein structures contain active peptide sequences, which can release bioactive peptides with various functions through in vitro hydrolysis or in vivo digestive enzymes. Among them, food-derived ACE inhibitor peptides have great potential in the prevention and treatment of hypertension. Food-derived ACE inhibitor peptide has the advantages of being safe to eat and has no side effects, and has become an important functional food factor for the adjuvant treatment of hypertensive patients. ACE is a metallopeptidase containing two Zn-binding 2+ The site, the so-called "must bind site", also contains one or several "additional binding...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61P9/12C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
Inventor 朱振宝易建华周慧江刘旷
Owner SHAANXI UNIV OF SCI & TECH
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