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Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease

A compound protease and walnut meal technology, which is applied in the field of food-derived protein deep processing, can solve the problems of product scale and industrial production limitations, limit the deep development and application of active peptides, and not reflect the use of final products, so as to improve immunity , Excellent room temperature storage stability and in vitro digestion stability, low cost effect

Inactive Publication Date: 2020-02-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are invention patents related to the technology of enzymatically hydrolyzing walnut protein to prepare walnut ACE inhibitory peptides, but there are some shortcomings, such as low product activity and no optimization of parameters, which limits the in-depth development and application of active peptides; some technologies only involve enzymatic hydrolysis The process does not reflect the use of the final product; some invented technologies are only suitable for small-scale production in laboratories, and have certain restrictions on the realization of large-scale and industrial production of products

Method used

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  • Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease
  • Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease
  • Method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease

Examples

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Effect test

Embodiment 1

[0058] A compound protease enzymatically hydrolyzes walnut dregs to prepare blood pressure-lowering walnut polypeptide and a method thereof. The specific steps are:

[0059] (1) Pretreatment of walnut meal: take fresh walnut meal after oil extraction, without oxidation and decay, pulverize, and ensure that the water content is below 8%, and pass through a 40-mesh sieve for subsequent use.

[0060] (2) Extraction of walnut protein: Weigh a certain amount of walnut meal powder to prepare a walnut meal powder solution, adjust the pH value to 9.0 with 1mol / L NaOH solution, ultrasonically extract for 1 hour, then centrifuge at 6500 rpm for 15 minutes, and discard the precipitate. Adjust the pH value of the supernatant to 4.5 with 1 mol / L HCl solution, centrifuge at 6500 rpm for 15 min, retain the precipitate, and wash with water until neutral. Vacuum freeze-drying and crushing to obtain walnut protein powder.

[0061] (3) Preparation of walnut polypeptide: Weigh a certain amount o...

Embodiment 2

[0063] A compound protease enzymatically hydrolyzes walnut dregs to prepare blood pressure-lowering walnut polypeptide and a method thereof. The specific steps are:

[0064] (1) Pretreatment of walnut meal: take fresh walnut meal after oil extraction, without oxidation and decay, pulverize, and ensure that the water content is below 8%, and pass through a 40-mesh sieve for subsequent use.

[0065] (2) Extraction of walnut protein: Weigh a certain amount of walnut meal powder to prepare a walnut meal powder solution, adjust the pH value to 9.0 with 1mol / L NaOH solution, ultrasonically extract for 1 hour, then centrifuge at 6500 rpm for 15 minutes, and discard the precipitate. Adjust the pH value of the supernatant to 4.5 with 1 mol / L HCl solution, centrifuge at 6500 rpm for 15 min, retain the precipitate, and wash with water until neutral. Vacuum freeze-drying and crushing to obtain walnut protein powder.

[0066] (3) Preparation of walnut polypeptide: Weigh a certain amount o...

Embodiment 3

[0068] A compound protease enzymatically hydrolyzes walnut dregs to prepare blood pressure-lowering walnut polypeptide and a method thereof. The specific steps are:

[0069] (1) Pretreatment of walnut meal: take fresh walnut meal after oil extraction, without oxidation and decay, pulverize, and ensure that the water content is below 8%, and pass through a 40-mesh sieve for subsequent use.

[0070] (2) Extraction of walnut protein: Weigh a certain amount of walnut meal powder to prepare a walnut meal powder solution, adjust the pH value to 8.0 with 1mol / L NaOH solution, ultrasonically extract for 1 hour, then centrifuge at 6500 rpm for 15 minutes, and discard the precipitate. Adjust the pH value of the supernatant to 4.5 with 1 mol / L HCl solution, centrifuge at 6500 rpm for 15 min, retain the precipitate, and wash with water until neutral. Vacuum freeze-drying and crushing to obtain walnut protein powder.

[0071] (3) Preparation of walnut polypeptide: Weigh a certain amount o...

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Abstract

The invention discloses a method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease, which belong to the technical field of food-borne protein deep processing.The protease preparation is screened and compounded, and enzymolysis process conditions are optimized, so that the walnut protein source antihypertensive peptide with high activity is prepared. The optimal process conditions for enzymolysis of walnut protein are as follows: the ratio of compound enzyme is 1: 2 (alkaline protease: neutral protease), the substrate concentration is 4%, the enzymolysis temperature is 45 DEG C, a pH value is 8.0, the enzyme addition amount is 8000U / g, and the enzymolysis time is 60min. The properties of polypeptide products are compared and analyzed, the relativemolecular mass of the products is mainly concentrated within a range of 0-2 kDa, good normal-temperature storage stability and in-vitro digestion stability are achieved, and a theoretical basis is provided for industrial production and practical application of the products.

Description

technical field [0001] The invention relates to a method for preparing hypotensive peptides by enzymatically hydrolyzing walnut dregs with compound protease, and belongs to the technical field of food-derived protein deep processing. Background technique [0002] Hypertension is a major risk factor for cardiovascular disease and one of the major diseases leading to human death. In recent years, the mortality rate of cardiovascular diseases in my country has continued to rise, ranking first among Chinese residents. With the continuous increase of hypertensive patients in our country, it is of great significance to strengthen the prevention and treatment of hypertension to improve human health and prolong life. Since drug treatment has certain side effects, it is a research hotspot in the non-drug treatment of hypertension to develop safe, effective, less side effects, low-cost active factors with hypotensive effect derived from natural food. Food-derived angiotensin-convert...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 孙秀兰叶永丽祝素莹张银志耿树香宁德鲁
Owner JIANGNAN UNIV
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