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A kind of preparation method of casein hypotensive peptide

A blood pressure-lowering peptide and casein technology, which is applied in the preparation and preliminary separation of peptides, can solve the problems of side effects, blood pressure rebound, and short duration of action, and achieve high ACE inhibitory activity, easy absorption in the body, and simple and easy process Effect

Inactive Publication Date: 2011-12-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a variety of chemically synthesized peptides can inhibit ACE activity, they all have certain side effects, and because their action time is not long, blood pressure will rebound after drug withdrawal, and a large number of experiments have proved that ACE inhibitory peptides derived from milk protein Has a high antihypertensive effect in vivo, mainly concentrated in small molecule peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of a casein blood pressure-lowering peptide of the present invention uses casein as a raw material, adds 100 mL of water for every 1.5 g of casein, maintains at 85° C. to 95° C. for 10 minutes to denature the casein, and adjusts the pH to 1.8 after cooling to room temperature. The hydrolysis temperature is 37°C, add pepsin 29000U per 1g of casein, hydrolyze for 2h, keep at 80°C-90°C for 10min to inactivate the enzyme, cool to room temperature and centrifuge at 4000r / min, adjust the supernatant to pH=7.8, hydrolyze The temperature is 48°C, add trypsin 15000U and chymotrypsin 15000U per 1g of casein, hydrolyze for 5h, keep at 80°C-90°C for 10min to inactivate the enzyme, cool to room temperature and centrifuge at 4000r / min, vacuum the supernatant Concentrated and freeze-dried to obtain white or light yellow powder casein hypotensive peptide crude product. Take the crude product of casein hypotensive peptide to prepare a 20mg / ml polypeptide solution,...

Embodiment 2

[0019] Similar to Example 1, the difference is that 100 mL of water is added for every 3 g of casein, pH=2.0, 20000 U of pepsin is added for every 1 g of casein, and the hydrolysis time is 3 h.

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PUM

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Abstract

The invention discloses a preparation method of casein hypotensive peptide and belongs to the field of biotechnology. The method comprises the following steps: heat and denature the casein suspension, adjust the pH after cooling, add pepsin, inactivate the enzyme after the reaction is completed, centrifuge, adjust the pH of the supernatant, and then add trypsin and chymotrypsin to continue the reaction. After completion, the enzyme was inactivated, centrifuged, and the supernatant was vacuum concentrated and freeze-dried to obtain the crude casein hypotensive peptide, which was separated by macroporous adsorption resin and Sephadex chromatography to obtain the casein hypotensive peptide with ACE inhibitory activity . The method simulates gastrointestinal digestion and utilizes two-step enzymatic hydrolysis to prepare small molecular peptides, which have high ACE inhibitory activity, no toxic and side effects, and are easy to absorb in vivo, and can be used to prepare antihypertensive drugs and food.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, in particular to the preparation and primary separation technology of polypeptides. Background technique: [0002] Antihypertensive peptides, also known as ACE inhibitory peptides (Angiotensin-converting Enzyme Inhibitory Peptides, ACEIP) is a class of small molecule peptides that can lower blood pressure in humans, it is an inhibitor of angiotensin-converting enzyme (Angiotensin-converting Enzyme, ACE) , can lower blood pressure by inhibiting the conversion of angiotensin I to angiotensin II and preventing ACE from degrading bradykinin. Although a variety of chemically synthesized peptides can inhibit ACE activity, they all have certain side effects, and because their action time is not long, blood pressure will rebound after drug withdrawal, and a large number of experiments have proved that ACE inhibitory peptides derived from milk protein It has a high antihypertensive effect in vivo, mainly co...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K4/12C07K1/36C07K1/22C07K1/16A61P9/12
Inventor 吕兵王桂春陆剑平王剑峰
Owner JIANGNAN UNIV
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