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Method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides

A fish skin collagen and inhibitory peptide technology, which is applied in the field of food biochemistry, can solve the problems that have not yet been reported on the study of catfish skin collagen ACE inhibitory peptides, and achieve the effect of low price, wide range of sources, and improved ACE inhibitory activity

Inactive Publication Date: 2014-02-12
广西农垦昌菱农场有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the ACE inhibitory peptide of catfish skin collagen

Method used

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  • Method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The catfish skin collagen was formulated into a solution with a substrate concentration of 1% (w / v, g / mL), the pH was adjusted to 7.0, and α-chymotrypsin (derived from bovine pancreas) was used for enzymolysis at 40°C for 10 minutes. The amount of enzyme added is 1000U / g. After the enzymolysis is completed, the enzyme is inactivated in a boiling water bath, the pH of the reaction mixture is adjusted to 8.0-9.0, and the enzyme is hydrolyzed with Alcalase (Novozymes food grade alcalase 2.4L) at 50°C for 20 minutes, and then added The amount of enzyme is 2000U / g. After the enzymolysis is completed, the enzyme is killed in a boiling water bath, the supernatant is collected by centrifugation, the pH of the supernatant is adjusted to neutral, and the concentration of the supernatant is concentrated to a solid content of 10% (w / v, g / mL), spray-dried at an air inlet temperature of 180°C and an outlet air temperature of 90°C to obtain a powdered catfish skin collagen ACE inhibit...

Embodiment 2

[0021] The catfish skin collagen was prepared into a solution with a substrate concentration of 2% (w / v, g / L), the pH was adjusted to 7.5, and α-chymotrypsin (sourced from bovine pancreas) was used for enzymolysis at 45°C for 15 minutes. The amount of enzyme added is 2000 / g. After the enzymolysis is completed, the enzyme is inactivated in a boiling water bath, the pH of the reaction mixture is adjusted to 8.5, and the enzyme is hydrolyzed with Alcalase (Novozymes food grade alcalase 2.4L) at 55°C for 25 minutes. 2500U / g, after the end of enzymolysis, boil the water bath to kill the enzyme, centrifuge to collect the supernatant, adjust the pH of the supernatant to neutral, and concentrate with a vacuum concentration device to a solid content of 10% (w / v, g / mL ) around 195°C at the inlet air temperature and 100°C at the outlet air temperature to obtain the powdered catfish skin collagen ACE inhibitory peptide product, and use ethyl acetate extraction-colorimetric method to determ...

Embodiment 3

[0023] The catfish skin collagen was formulated into a solution with a substrate concentration of 3% (w / v, g / L), the pH was adjusted to 8.0, and α-chymotrypsin (sourced from bovine pancreas) was used for enzymolysis at 50°C for 20 minutes. The amount of enzyme added is 3000U / g. After the enzymolysis is completed, the enzyme is inactivated in a boiling water bath. 3000U / g, after the enzymolysis, boil the water bath to kill the enzyme, centrifuge to collect the supernatant, adjust the pH of the supernatant to neutral, and concentrate it with a vacuum concentration device to a solid content of 10% (w / v, g / mL ) around 210°C at the inlet air temperature and 110°C at the outlet air temperature to obtain the powdered catfish skin collagen ACE inhibitory peptide product, and use ethyl acetate extraction-colorimetric method to determine different concentrations of catfish skin collagen Activity of protein ACE-inhibiting peptides, IC of catfish skin collagen ACE-inhibiting peptides dete...

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Abstract

The invention discloses a method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides. The method comprises the following steps: preparing channel catfish skin collagen into a solution with a substrate concentration of 1-3%, adjusting the pH value of the solution to 7.0-8.0; carrying out enzymolysis on the solution for 10-20 min at a temperature of 40-50 DEG C by using alpha-chymotrypsin, wherein the addition amount of the alpha-chymotrypsin is 1000-3000U / g; after the enzymolysis is completed, carrying out enzyme deactivation by using a boiling water bath; adjusting the pH value of reaction mixed liquid to 8.0-9.0; carrying out enzymolysis on the reaction mixed liquid for 20-30min at a temperature of 50-60 DEG C by using Alcalase enzyme, wherein the addition amount of the Alcalase enzyme is 2000-3000U / g; and after the enzymolysis is completed, carrying out enzyme deactivation by using the boiling water bath, and centrifugally collecting liquid supernatant. According to the method, channel catfish skin is used for preparing collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides for the first time, thereby realizing the efficient utilization of channel catfish skin resources; a defect that loca of single enzymatic hydrolysis are insufficient, and loca of mixed enzymatic hydrolysis are interfered with each other is overcome, the ACE inhibitory activity of enzymolysis products is improved, and the IC50 value is 0.33-0.86 mg / mL.

Description

technical field [0001] The invention relates to the field of food biochemistry and can be used as a functional additive in food, cosmetics and medicines, in particular to a preparation method of catfish skin collagen ACE inhibitory peptide. Background technique [0002] Food-source ACE inhibitory peptides have become one of the hot research fields for researchers because of their safety and no side effects. Now from grass carp (Liu X, Zhang MS, Jia AR, et al.Purification and characterization of angiotensin I converting enzyme inhibitory peptides from jellyfish Rhopilema esculentum[J].Food Research International,2013,50(1):339-343) , jellyfish (Ishiguro K, Sameshima Y, Kume T, et al.Hypotensive effect of a sweetpotato protein digest in spontaneously hypertensive rats and purification of angiotensin I-converting enzyme inhibitory peptides[J].Food Chemistry,2012,131(3): 774-779) and other aquatic products were isolated from the hydrolyzate of high activity ACE inhibitory pepti...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 吴琼英贾俊强颜辉桂仲争
Owner 广西农垦昌菱农场有限公司
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