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Method for preparing antihypertensive active peptides

A technology of active peptides and antihypertensive peptides, which is applied in the field of preparation of antihypertensive active peptides, can solve problems such as loss of ACE inhibitory effect, antihypertensive peptides are not resistant to digestion, application limitations, etc., and achieve high ACE inhibitory activity, no toxic side effects, Good antihypertensive effect

Inactive Publication Date: 2019-02-19
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wang Haiying and others used low-temperature alkaline protease from marine microorganisms as a degradation tool to act on deep-sea cod skin to prepare blood pressure-lowering peptides with angiotensin-converting enzyme inhibitor activity (Wang Haiying, Song Yongxiang, Sun Mi. Enzymatic hydrolysis of cod skin to prepare angiotensin Research on converting enzyme inhibitor ACEI[J].Modern Food Science and Technology, 2008(10):987-991.) However, most of the above-mentioned antihypertensive peptides from food sources are not resistant to digestion, and are easily decomposed by digestive enzymes in the body, losing their ability to ACE inhibition
Therefore, the application is subject to certain restrictions

Method used

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  • Method for preparing antihypertensive active peptides
  • Method for preparing antihypertensive active peptides
  • Method for preparing antihypertensive active peptides

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Experimental program
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Embodiment 1

[0019] Precisely weigh 24g of cod oligopeptides with a molecular weight of 500-1000Da or cod protein peptides with a molecular weight of 1000-3000Da, dissolve them in 600 mL of deionized water, and stir to completely dissolve to obtain a concentration of 4% (W / V). oligopeptide / protein peptide solution. Take 18 50 mL centrifuge tubes to mark the type and concentration of enzymes (6 different proteases, 3 dosages for each enzyme). Take another 500 mL large beaker, add 300 mL of the oligopeptide / protein peptide solution with a concentration of 4% prepared above, use sodium hydroxide or hydrochloric acid solution and use a pH meter to adjust the pH value of the peptide solution to 7.0, and accurately measure Take 30 mL of the peptide solution with pH 7.0 and transfer it to No. 1-6 centrifuge tubes and No. 10-12 centrifuge tubes; then take a 500 mL large beaker, add 200 mL of the prepared oligopeptide / protein peptide solution above, and use Sodium hydroxide or hydrochloric acid so...

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Abstract

The invention relates to the field of active peptide preparation, in particular to a method for preparing antihypertensive active peptides. The method includes carrying out enzymatic hydrolysis on Alaska cod by enzymes; carrying out enzyme deactivation on enzymatic hydrolysate, and then carrying out centrifuging and filtering to obtain the 500-3000 D antihypertensive active peptides which are antihypertensive peptides. The Alaska cod is used as a raw material. The method has the advantages that the antihypertensive active peptides prepared from the Alaska cod by the aid of the method are highin ACE (angiotensin converting enzyme) inhibitory activity and free of toxic and side effect, and can be easily absorbed and utilized by human bodies, and good antihypertensive effects can be realizedby the antihypertensive active peptides; the basis can be provided to comprehensively utilizing cod resources, and the basis can be provided to further developing natural antihypertensive healthcareproducts.

Description

technical field [0001] The invention relates to the field of preparation of active peptides, in particular to a preparation method of antihypertensive active peptides. Background technique [0002] Hypertension is currently the most prevalent cardiovascular disease and one of the major chronic diseases that endanger human health. In the blood pressure regulation system, angiotensin-converting enzyme (ACE) can hydrolyze angiotensin I to generate angiotensin II, which can increase blood pressure. It can also hydrolyze bradykinin, making it lose The effect of lowering blood pressure. Antihypertensive peptides from food-derived proteins, namely angiotensin I-conberting enzyme inhibitory peptides (ACEI), are more and more popular due to their high safety and few side effects. At present, people have obtained peptide fragments with ACE inhibitory activity from plant protein, animal protein, aquatic protein, and milk protein hydrolyzate. Marine products such as cod have a good p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 李文军杨贵兰秦松刘正一王明超
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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