Preparation method and application of urechis unicinctus ACE inhibitory peptide
A single-ring stinger and inhibitory peptide technology, applied in the field of biomedicine, can solve the problems of records and reports of ACE inhibitory peptides
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0045] The invention provides a method for preparing a monocyclic spiny ACE inhibitory peptide, comprising the following steps:
[0046] 1) Degreasing the crushed C. monoringensis to obtain a degreasing product; drying and pulverizing the degreasing product to obtain a body wall powder of C. monoringensis;
[0047] 2) Mixing the body wall powder, neutral protease and water of the monocyclic spinyweed, performing an enzymatic hydrolysis reaction, deactivating the enzyme in the enzymatic hydrolysis reaction liquid, separating the solid from the liquid, and collecting the liquid components, which contain the monocyclic ACE-inhibiting peptides of the spiny cartilage;
[0048] The mass-to-volume ratio of the body wall powder and water of the single-ring echinacea described in step 2) is 1 g: (20-30) mL;
[0049] In step 2), the mass ratio of the body wall powder and neutral protease of C. monoringensis is 100: (2-4);
[0050] The time for the enzymatic hydrolysis reaction in step...
Embodiment 1
[0083] 1. Thaw the frozen C. monoringensis with running water at room temperature, dry the surface moisture at low temperature, dissect and mince, soak in ethyl acetate for 48 hours, and keep stirring during the degreasing process. The degreased sample was vacuum filtered to remove ethyl acetate (recovered by rotary evaporation), dried and pulverized.
[0084] 2. Weigh an appropriate amount of pretreated C. monoringensis body wall powder, add deionized water at a solid-liquid ratio of 1g: 20mL, and add enzyme at a mass concentration of 2%, enzymatically hydrolyze for 4 hours, and use 0.5mol / L NaOH and 0.1mol / L HCL solution to adjust the pH, and neutral protease (45°C, pH 7.0) for enzymatic hydrolysis. The enzymolysis solution was inactivated in a water bath at 100°C by heating to inactivate the enzyme. After inactivating the enzyme, cool to room temperature, centrifuge at 8000r / min for 10min, collect the supernatant containing the ACE-inhibiting peptide of the monocyclonic sp...
Embodiment 2
[0094] Except that the enzyme amount of the neutral protease in Example 1 is replaced by 3%, all the other are the same as Example 1.
PUM
Property | Measurement | Unit |
---|---|---|
Molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com