Preparation method and application of urechis unicinctus ACE inhibitory peptide

A single-ring stinger and inhibitory peptide technology, applied in the field of biomedicine, can solve the problems of records and reports of ACE inhibitory peptides

Inactive Publication Date: 2021-03-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, in the prior art, there is no record and report about the preparation of ACE inhibitory peptides derived from the monocyclic spiny cartilage

Method used

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  • Preparation method and application of urechis unicinctus ACE inhibitory peptide
  • Preparation method and application of urechis unicinctus ACE inhibitory peptide
  • Preparation method and application of urechis unicinctus ACE inhibitory peptide

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preparation example Construction

[0045] The invention provides a method for preparing a monocyclic spiny ACE inhibitory peptide, comprising the following steps:

[0046] 1) Degreasing the crushed C. monoringensis to obtain a degreasing product; drying and pulverizing the degreasing product to obtain a body wall powder of C. monoringensis;

[0047] 2) Mixing the body wall powder, neutral protease and water of the monocyclic spinyweed, performing an enzymatic hydrolysis reaction, deactivating the enzyme in the enzymatic hydrolysis reaction liquid, separating the solid from the liquid, and collecting the liquid components, which contain the monocyclic ACE-inhibiting peptides of the spiny cartilage;

[0048] The mass-to-volume ratio of the body wall powder and water of the single-ring echinacea described in step 2) is 1 g: (20-30) mL;

[0049] In step 2), the mass ratio of the body wall powder and neutral protease of C. monoringensis is 100: (2-4);

[0050] The time for the enzymatic hydrolysis reaction in step...

Embodiment 1

[0083] 1. Thaw the frozen C. monoringensis with running water at room temperature, dry the surface moisture at low temperature, dissect and mince, soak in ethyl acetate for 48 hours, and keep stirring during the degreasing process. The degreased sample was vacuum filtered to remove ethyl acetate (recovered by rotary evaporation), dried and pulverized.

[0084] 2. Weigh an appropriate amount of pretreated C. monoringensis body wall powder, add deionized water at a solid-liquid ratio of 1g: 20mL, and add enzyme at a mass concentration of 2%, enzymatically hydrolyze for 4 hours, and use 0.5mol / L NaOH and 0.1mol / L HCL solution to adjust the pH, and neutral protease (45°C, pH 7.0) for enzymatic hydrolysis. The enzymolysis solution was inactivated in a water bath at 100°C by heating to inactivate the enzyme. After inactivating the enzyme, cool to room temperature, centrifuge at 8000r / min for 10min, collect the supernatant containing the ACE-inhibiting peptide of the monocyclonic sp...

Embodiment 2

[0094] Except that the enzyme amount of the neutral protease in Example 1 is replaced by 3%, all the other are the same as Example 1.

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Abstract

The invention provides a preparation method and application of urechis unicinctus ACE inhibitory peptide, and belongs to the technical field of biological medicine. The preparation method comprises the following steps of degreasing crushed urechis unicinctus, and obtaining a degreased product; drying and crushing the degreased product to obtain urechis unicinctus body wall powder; and mixing the urechis unicinctus body wall powder, neutral protease and water, performing enzymolysis reaction, enzyme deactivation and centrifugation, and collecting supernatant containing the urechis unicinctus ACE inhibitory peptide. The urechis unicinctus ACE inhibitory peptide prepared by the method provided by the invention has good ACE inhibitory activity, and the ACE inhibitory rate of the supernatant containing the urechis unicinctus ACE inhibitory peptide is 52.84 + / -0.31% through measurement.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of a monocyclic spiny ACE inhibitory peptide. Background technique [0002] Hypertension is a condition in which the pressure in blood vessels continues to rise. This disease greatly increases the risk of heart, brain, kidney and other organs, and also greatly increases the risk of other diseases. [0003] Angiotensin-converting enzyme (ACE) plays an important role in regulating blood pressure by converting angiotensin I to the octapeptide angiotensin II, which has a strong vasoconstrictive effect, and degrades bradykinin through vasodilation. ACE inhibitory peptides can compete with the two active functional domains of ACE, inhibit ACE activity, and inhibit the conversion of angiotensin I to angiotensin II. At present, ACE inhibitory peptides are generally extracted directly from food, or obtained by hydrolyzing proteins with food-grade ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/435C07K1/36C07K1/34C07K1/20C07K1/16C07K1/14A61K38/01A61K38/17A61P9/12
CPCC12P21/06C07K14/43536A61P9/12A61K38/00
Inventor 孙坤来李佩佩初雪梅陈荫王斌赵玉勤王玉梅张译文
Owner ZHEJIANG OCEAN UNIV
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