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Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof

A technology of collagen polypeptide and yak skin, applied in the field of collagen polypeptide and its preparation, can solve problems such as side effects, achieve good solubility, simple preparation method, and high ACE inhibitory activity

Inactive Publication Date: 2014-10-08
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, synthetic ACE inhibitory compounds generally have side effects, such as cough, dysgeusia, rash and angioedema, etc.

Method used

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  • Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] (1) Wash the yak skin in water to remove surface impurities, then cut off the hair on the dried yak skin, and then soak the yak skin in warm water at 80°C for 20 hours, remove the hair thoroughly mechanically, rinse with clean water, remove the dandruff, and cut the skin Cut into small pieces of 0.5×0.5cm to obtain the depilated yak hide, and store it in a freezer at -18~-20°C;

[0026] (2) Place the depilated yak hide in 12% n-butanol, the ratio of solid to liquid is 1:10, and continuously stir for 20 hours at a solution temperature of 4° C. to obtain degreased yak hide;

[0027] (3) Put the degreased yak hide in the alkali solution with a molar concentration of 0.1M at a ratio of 1:15, and stir continuously at 4°C for 20 hours to obtain the yak hide without impurities;

[0028] (4) Put the impurity-removed protein yak hide in 0.2% acid solution, the ratio of solid to liquid is 1:10, stir and soak at room temperature for 5 hours, then repeatedly wash the yak hide until...

example 2

[0035] (1) Wash the yak skin in water to remove surface impurities, then cut off the hair on the dried yak skin, then soak the yak skin in warm water at 80°C for 10 hours, remove the hair thoroughly mechanically, rinse with clean water, remove the dandruff, and cut the skin Cut into small pieces of 0.5×0.5cm to obtain the depilated yak hide, and store it in a freezer at -18~-20°C;

[0036] (2) Put the dehaired yak hide in 5% n-butanol, the ratio of solid to liquid is 1:5, and continuously stir for 12 hours at the temperature of the solution at 4° C. to obtain degreased yak hide;

[0037] (3) Put the degreased yak hide in the alkali solution with a molar concentration of 0.1M at a ratio of 1:20, and stir continuously at 5°C for 20 hours to obtain the yak hide without impurities;

[0038] (4) Put the impurity-removed protein yak skin in 1% acid solution, the ratio of material to liquid is 1:5, stir and soak at room temperature for 5 hours, then repeatedly wash the yak skin until t...

example 3

[0045] (1) Wash the yak skin in water to remove surface impurities, then cut off the hair on the dried yak skin, and then soak the yak skin in warm water at 60°C for 4 hours, mechanically depilate, rinse with clean water, remove dander, and cut the skin Cut into small pieces of 0.5×0.5cm to obtain the depilated yak hide, and store it in a freezer at -18~-20°C;

[0046] (2) Place the depilated yak hide in 10% n-butanol, the ratio of solid to liquid is 1:10, and continuously stir for 12 hours at a solution temperature of 4° C. to obtain degreased yak hide;

[0047] (3) Put the degreased yak hide in the lye solution with a molar concentration of 0.2M at a ratio of 1:40, and stir continuously at 4°C for 20 hours to obtain the yak hide without impurities;

[0048] (4) Put the impurity-removed protein yak skin in 2% acid solution, the ratio of material to liquid is 1:5, stir and soak at room temperature for 5 hours, then repeatedly wash the yak skin until the pH value reaches 6, and...

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Abstract

The invention discloses a yak skin blood pressure-decreasing collagen polypeptide and a preparation method thereof. The preparation comprises the following steps: pre-treating raw materials by virtue of physical and chemical methods, preparing gelatin, preparing ACE-inhibiting collagen polypeptide by virtue of composite enzymatic hydrolysis, and separating and purifying polypeptide by utilizing methods such as ultra-filtration, ion-exchange column chromatography, gel filtration chromatography and high performance liquid chromatography; concentrating and drying. According to the technical scheme in the invention, collagen polypeptide is successfully extracted from the yak skin, and the collagen polypeptide has good solubility, emulsifying capacity and foaming characteristic, and has higher ACE-inhibiting activity (blood pressure decreasing effect); the preparation method is simple and easy to operate, and low in cost, so that the application prospect of the yak skin is expanded.

Description

technical field [0001] The invention relates to a collagen polypeptide and a preparation method thereof, more specifically, to a yak skin hypotensive polypeptide and a preparation method thereof. Background technique [0002] Collagen peptides are usually products obtained by degradation of collagen or gelatin by protease, etc., and the amino acids contained are basically the same as those of collagen, especially the characteristic amino acid hydroxyproline. The research on modern biological metabolism has found that through a series of enzymes digesting protein, some small molecular substances can be formed and absorbed by the human body. Absorption in the body is not limited to the form of free amino acids, but mainly directly absorbed in the form of small peptides composed of 2-3 amino acids. Antihypertensive peptides are also known as angiotensin converting enzyme (ACE) inhibitory peptides. ACE inhibitory peptide hinders the catalytic reaction of ACE through competitiv...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34A61P9/12
Inventor 朱宏吉马力王世鹏
Owner TIANJIN UNIV
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