Novel peptide with angiotonin transferase restraining liveness and method of producing the same

A polypeptide, basic technology, applied in the field of novel peptides with angiotensin transferase inhibitory activity and their preparation

Inactive Publication Date: 2008-04-02
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many ACE inhibitors (captopril, etc.) have been sold on the market. Although the effect of this type of synthetic drugs on reducing hypertension is significant, the adverse reactions in long-term use show that there is still a need for antihypertensive therapeutic agents without side effects or non-toxic drugs. Drug Assisted Therapy Preparations

Method used

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  • Novel peptide with angiotonin transferase restraining liveness and method of producing the same
  • Novel peptide with angiotonin transferase restraining liveness and method of producing the same

Examples

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Embodiment 1

[0058] Preparation of peptides and their biological activity

[0059] Choose fresh cowhide that has passed the quarantine, and 100g of cowhide fine pieces after pretreatment such as hair removal, degreasing, and non-collagen removal, soak in 1.5L of potassium chloride-hydrochloric acid buffer solution with a pH of 1.0 and an ionic strength of 0.05M Keep stirring for 6 hours to make the skin swell. After the swelling is completed, homogenize intermittently for 3 minutes at a speed of 12,000 rpm in a tissue masher, then heat and denature the homogenate in a boiling water bath for 3 minutes, and homogenize again for 2 minutes;

[0060] Adjust the above homogenate to pH 1.5, temperature 38°C, add 1:3000 pepsin (final concentration 1%), use the stirring paddle to continue stirring at a speed of 120rpm, stop the reaction after 4 hours, add Tris base to make the final concentration Reach 0.05M, use 10N NaOH solution to adjust the pH of the solution to reach pH8.0, heat up to 55°C, ad...

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Abstract

The present invention discloses a method of using biological enzyme decomposition to prepare a hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity, and three novel peptides of high ACE inhibitory activity. For the hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity of the present invention, the oxhide rich in praline is used as the raw material; the product of ACE inhibitory activity IC50 equal to 0.168mg / m can be prepared through the compounding and hydrolysis of biological enzyme. After ultrafiltration, the IC50 is equal to 0.079mg / ml. The means of analysis and detection determines the peptides, ISVPGPM, LGPVGNPGPA and DLSFLPQPPQQ, containing high ACE inhibitory activity. The three peptide sections are novel peptide sections containing high ACE inhibitory activity. Therefore, the three peptides have potential function of lowering blood pressure. The compounding and decomposition of biological enzyme of oxhide is a peptide type hydrolysis product, has inhibitory effects on angiotensin transfer enzyme (ACE), and can be widely used for health food, functional food, auxiliary drugs for lowering blood pressure and other products; the peptide has broad market prospects.

Description

technical field [0001] The invention relates to a preparation method of a peptide with angiotensin transferase inhibitory activity, in particular to a biocomposite enzymatic hydrolysis method, and three new ACE inhibitory active peptides prepared by the method, which have the function of lowering blood pressure. Background technique [0002] Hypertension is a common and frequently-occurring disease worldwide. Since drug treatment of hypertension can cause various adverse reactions, antihypertensive peptides extracted from natural proteins with high food safety and no side effects have increasingly become the most promising products for non-drug treatment of hypertension. [0003] Angiotensin-converting enzyme (abbreviated as ACE, dipeptide hydroxypeptidase, EC3, 4, 15, 1) can catalyze the conversion of angiotensin I into angiotensin II, thereby leading to an increase in blood pressure. Therefore, inhibiting the activity of ACE becomes a means of regulating blood pressure. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61K38/10A61P9/12
Inventor 沈亚领马志英严维凌沈菊泉魏东芝董莹汤俊谢志镭任莉萍鲍熹珺刘伯雅
Owner EAST CHINA UNIV OF SCI & TECH
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