Novel peptide with angiotonin transferase restraining liveness and method of producing the same
A polypeptide, basic technology, applied in the field of novel peptides with angiotensin transferase inhibitory activity and their preparation
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[0058] Preparation of peptides and their biological activity
[0059] Choose fresh cowhide that has passed the quarantine, and 100g of cowhide fine pieces after pretreatment such as hair removal, degreasing, and non-collagen removal, soak in 1.5L of potassium chloride-hydrochloric acid buffer solution with a pH of 1.0 and an ionic strength of 0.05M Keep stirring for 6 hours to make the skin swell. After the swelling is completed, homogenize intermittently for 3 minutes at a speed of 12,000 rpm in a tissue masher, then heat and denature the homogenate in a boiling water bath for 3 minutes, and homogenize again for 2 minutes;
[0060] Adjust the above homogenate to pH 1.5, temperature 38°C, add 1:3000 pepsin (final concentration 1%), use the stirring paddle to continue stirring at a speed of 120rpm, stop the reaction after 4 hours, add Tris base to make the final concentration Reach 0.05M, use 10N NaOH solution to adjust the pH of the solution to reach pH8.0, heat up to 55°C, ad...
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