Urechis unicinctus biological active peptide and preparation method thereof

A technology of bioactive peptides and monocyclic thorns, applied in the field of bioactive peptides, can solve problems such as impact, side effects, and adverse effects, and achieve the effects of inhibiting proliferation, good ACE inhibitory activity, and good ACE inhibitory effect

Active Publication Date: 2018-01-12
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these treatments usually require life-long treatment with varying degrees of adverse effects and side effects

Method used

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  • Urechis unicinctus biological active peptide and preparation method thereof
  • Urechis unicinctus biological active peptide and preparation method thereof
  • Urechis unicinctus biological active peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A monocyclic echinacea bioactive peptide, the amino acid sequence of which is Thr-Arg-Gly-Pro-Pro.

[0040] A method for preparing a bioactive peptide from the monocyclic thorn, comprising the following steps:

[0041] a) shredding and degreasing: chopping the body wall of C. unicircularis, and degreasing treatment with acetone, centrifuging to remove the supernatant after degreasing, and obtaining the body wall powder of C. unicircularis after drying;

[0042] b) Preparation of crude product: Mix the body wall powder of C. monocircleum with water so that the mass concentration of C. monocycle body wall powder is 0.04g / ml, then heat at 75°C for 4 hours, then centrifuge to take the supernatant, and prepare get crude products;

[0043] b) Initial enzymolysis: Add 4.5ml of water per 1g of the crude product, mix the crude product with water, adjust the pH to 6.5, add 2wt% papain and heat at 55°C for 4 hours, then Heat at 100°C for 15 minutes to inactivate the enzyme, and ...

Embodiment 2

[0058] A monocyclic echinacea bioactive peptide, the amino acid sequence of which is Thr-Arg-Gly-Pro-Pro.

[0059] A method for preparing a bioactive peptide from the monocyclic thorn, comprising the following steps:

[0060] a) shredding and degreasing: chopping the body wall of C. unicircularis, and degreasing treatment with acetone, centrifuging to remove the supernatant after degreasing, and obtaining the body wall powder of C. unicircularis after drying;

[0061] b) Preparation of crude product: Mix the body wall powder of C. monocircleum with water so that the mass concentration of C. monocycle body wall powder is 0.05g / ml, then heat at 80°C for 4 hours, then centrifuge to take the supernatant, and prepare get crude products;

[0062] b) Initial enzymatic hydrolysis: Add 5.0ml of water per 1g of the crude product, mix the crude product with water, adjust the pH to 6.8, add 2.5wt% papain and heat at 60°C for 4 hours, Then heat at 100°C for 15 minutes to inactivate the e...

Embodiment 3

[0077] A monocyclic echinacea bioactive peptide, the amino acid sequence of which is Thr-Arg-Gly-Pro-Pro.

[0078] A method for preparing a bioactive peptide from the monocyclic thorn, comprising the following steps:

[0079] a) shredding and degreasing: chopping the body wall of C. unicircularis, and degreasing treatment with acetone, centrifuging to remove the supernatant after degreasing, and obtaining the body wall powder of C. unicircularis after drying;

[0080] b) Preparation of the crude product: mix the body wall powder of C. monocircleum with water so that the mass concentration of the body wall powder of C. monocircleum is 0.06 g / ml, then heat at 85° C. for 4 hours, then centrifuge to take the supernatant, and prepare get crude products;

[0081] b) Initial enzymatic hydrolysis: Add 5.5ml of water per 1g of the crude product, mix the crude product with water, adjust the pH to 7.0, add 3wt% papain and heat at 65°C for 4 hours, then Heat at 100°C for 15 minutes to i...

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Abstract

The invention belongs to the technical field of biological active peptide. The invention discloses urechis unicinctus biological active peptide, wherein the amino acid sequence is Thr-Arg-Gly-Pro-Pro.The invention also discloses a preparation method of the urechis unicinctus biological active peptide, which is a method for extracting the urechis unicinctus biological active peptide from the bodywall of the urechis unicinctus. The urechis unicinctus biological active peptide has pressure-reducing activity, has high angiotensin converting enzyme (ACE) inhibition activity and can inhibit proliferation of vascular endothelial cells.

Description

technical field [0001] The invention relates to the technical field of biologically active peptides, in particular to a biologically active peptide from the monocyclic echinacea and a preparation method thereof. Background technique [0002] Single-ring spinyweed, common name: sea intestine, sea intestine, belongs to the phylum of scorpion animals, class of scorpion, order of scorpion, family of scorpion, genus of scorpion; individual hypertrophy, delicious meat, body wall muscle rich in protein and many An essential amino acid for the human body. Since ancient times, it has been used as a famous and precious seafood in the coastal areas of my country, Japan and North Korea, with high economic value. Traditionally, people only eat the body wall and discard the internal organs. The dish is commonly known as sea intestines. The study found that the protein content of Echinacea monoringensis is 18.25%, the fat content is 0.12%, the total sugar content is 4.09%, and it is rich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/20A61P9/12A61P7/00
Inventor 李佩佩张小军严忠雍陈荫龙举方益
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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