Novel loach protein antihypertensive peptide and preparation method thereof

A technology of antihypertensive peptide and blood pressure lowering peptide, which is applied to the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problem that the research of loach protein blood pressure lowering peptide has not been reported, etc., and achieves good medicinal value and low price. , the effect of high food safety

Active Publication Date: 2011-09-21
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and application of loach are limited to the research on the antioxidant peptide of loach protein and the ant...

Method used

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  • Novel loach protein antihypertensive peptide and preparation method thereof
  • Novel loach protein antihypertensive peptide and preparation method thereof
  • Novel loach protein antihypertensive peptide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1. The highly active ACE inhibitory peptide Leu-Glu-Tyr was isolated and purified from loach protein hydrolyzate.

[0032] (1) Enzymolysis of loach protein to prepare enzymatic solution

[0033] Grind loach meat into minced meat, add water to make a slurry, and then add 3‰ (w / w) bromelain (activity 3×10 5 U / g), adjust the pH value to 5.5, temperature 55°C, stir for 6 hours, inactivate the enzyme, centrifuge at 3000rpm for 20min, and the supernatant is the enzymolysis solution.

[0034] (2) Separation and purification of hypotensive peptides from enzymatic hydrolyzate

[0035] The enzymatic solution was decolorized with 2% (w / v) activated carbon for 20min, then passed through a 2500Da ultrafiltration membrane ultrafiltration, and the ultrafiltrate with an ultrafiltration of less than 2500Da was passed through a Sephadex G-15 column (1.6 × 100cm, Amersham Pharmacia Biotech , Piscataway, NJ, USA), flow rate 1.0mL / min, separated to obtain 5 peaks, the automatic fr...

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Abstract

The invention provides a loach protein antihypertensive peptide and a preparation method thereof, belonging to the technical field of biology. The antihypertensive short peptide separated and purified from an enzymolysis product of loach protein has high activity in inhibiting angiotensin converting enzyme (ACE). The preparation method provided by the invention comprises the following steps of: carrying out enzymolysis on the loach protein to prepare an enzymolysis liquid; separating and purifying the antihypertensive peptide from the enzymolysis liquid; and sequencing the antihypertensive peptide. The invention combines a modern enzyme engineering technique and a peptide purifying and identifying technique, deep development and utilization on the loach protein are utilized, and a novel-sequence short peptide Leu-Glue-Tyr with high ACE inhibiting activity is found; and the novel loach protein antihypertensive peptide provided by the invention has high ACE inhibiting activity and has an IC50 value of 0.103 mg/mL.

Description

1. Technical field [0001] The invention relates to an antihypertensive peptide with a new amino acid sequence isolated and purified from an enzymatic hydrolyzate of loach meat and its preparation method and application, which applies modern biotechnology to the deep processing of freshwater aquatic products, and belongs to the technical field of deep processing of freshwater aquatic products . 2. Background technology [0002] Most of the hydrolyzed products obtained by enzymatic hydrolysis of food-derived proteins are short peptides composed of several amino acid residues. These short peptides are absorbed into the circulatory system in a complete form and used by tissues. They have many physiological activities: anti-oxidation, improving Immunity, antibacterial and anti-inflammatory, promotion of passive transport of trace elements and storage in the body, antihypertensive activity, etc. [0003] Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxyl ...

Claims

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Application Information

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IPC IPC(8): C07K5/083A61P9/12C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 李莹周剑忠曾晓雄黄开红王英马艳弘张丽霞
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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