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Rapid preparation method of mussel protein antihypertensive peptide

A technology of antihypertensive peptides and mussels, which is applied in the field of bioengineering, can solve the problems of preparing antihypertensive peptides, and achieve the effect of improving utilization rate, significant ACE inhibitory activity and high activity

Active Publication Date: 2015-07-08
山东大树达孚特膳食品有限公司
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Problems solved by technology

However, the researchers found that there is no research on the preparation of antihypertensive peptides from mussel hydrolyzate by using this technology

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  • Rapid preparation method of mussel protein antihypertensive peptide

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Embodiment

[0024] A rapid preparation method of mussel protein antihypertensive peptide, the preparation process is as follows: mussel meat "tissue crushing" enzymatic hydrolysis "ultrafiltration" agarose magnetic microspheres immobilized ACE adsorption "RP-HPLC purification" antihypertensive peptide.

[0025] ) Preparation and activation of agarose magnetic microspheres: add agarose to water according to the weight-to-volume ratio of 1.5g: 30 mL, boil together until the agarose is completely dissolved, add 1 ml Fe 3 o 4 Ferrofluid, fully mixed on a vortex mixer; drop the mixed liquid into the organic phase at 80°C (Span-80: liquid paraffin=1g:25mL) under stirring, and react at 80°C for 20 min; the organic phase Cool to 40°C and mechanically stir at 250 rpm for 15 min to shape the microspheres, and separate them in a magnetic field. The prepared microspheres were washed three times with petroleum ether and deionized water to remove the residual organic phase to obtain agarose magnetic M...

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Abstract

The invention discloses a rapid preparation method of a mussel protein antihypertensive peptide. The antihypertensive peptide is characterized in that an amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp(VSWPCRW); the molecular weight determined by ESI-MS (electrospray ionization mass spectrometry) is 935.07Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp is obtained by shelling mussels, taking the meat of the mussels, carrying out enzymolysis on the meat, carrying out ultrafiltration on the enzymatic hydrolysate, adsorbing the enzymatic hydrolysate with an agarose magnetic microsphere immobilized ACE (angiotensin converting enzyme) and carrying out purification by RP-HPLC (reversed-phase high-performance liquid chromatography). The active peptide prepared by the invention has obvious ACE inhibitory activity and can be used in hypertension treatment related drugs.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a rapid preparation method of mussel protein antihypertensive peptide. Background technique [0002] Angiotensin-converting enzyme (ACE), also known as peptidyl-carboxypeptidase or kininase II, has a molecular weight of 150,000 Da and belongs to the membrane-bound enzyme of vascular endothelial cells. ACE can hydrolyze the decapeptide angiotensin I into the octapeptide angiotensin II, which promotes further constriction of blood vessels, leading to an increase in blood pressure. At the same time, ACE can also promote the secretion of aldosterone by acting on the adrenal cortex. Therefore, ACE is an important component of renin-angiotensin-aldosterone (RAS). In addition, ACE can also catalyze the hydrolysis of bradykinin, which has antihypertensive effect, and make it inactive. Therefore, the purpose of lowering or controlling blood pressure can be achieved b...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/22C07K1/20A61P9/12B01J13/02B01J20/24B01J20/30B01J20/28
Inventor 王斌迟长凤赵玉勤孙坤来
Owner 山东大树达孚特膳食品有限公司
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