35results about How to "Strong industrial implementation" patented technology

The invention discloses a method for preparing blood fat reducing peptide by use of silkworm chrysalis, which comprises the following steps: 1) mixing the degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder with water, regulating the pH value to 6.8-7.2, heating to 85-95 DEG C, preserving heat for 8-12 minutes and naturally cooling to 45-50 DEG C; adding papain for an enzymolysis reaction; heating the product of the enzymolysis reaction to 95-105 DEG C and keeping the temperature for 10-15 minutes; naturally cooling to room temperature to terminate the enzymolysis reaction; and centrifuging to obtain enzymolysis liquid; 2) performing decoloration treatment on the enzymolysis liquid at 42-48 DEG C by use of active carbon, and filtering the enzymolysis liquid after the decoloration; and 3) performing ultrafiltration treatment on the rough-filtration enzymolysis liquid under a working pressure of 0.1-0.2MPa and a working temperature of 0-45 DEG C by use of an ultrafiltration membrane to obtain ultrafiltration enzymolysis liquid; and sequentially performing vacuum concentration and freeze drying on the ultrafiltration enzymolysis liquid to obtain the blood fat reducing peptide.

The invention provides a freeze-drying protective agent as well as a preparation method and an application method thereof, in order to solve the problem on the preparation of the freeze-drying protective agent for different lactic acid bacteria strains in the prior art. According to the freeze-drying protective agent as well as the preparation method and the application method thereof disclosed by the invention, the freeze-drying protective agent is directly derived from corresponding lactic acid bacteria cells after freezing pretreatment at a low temperature, and is directly obtained by cell crushing treatment; and the freeze-drying protective agent disclosed by the invention has the advantages of easy availability for raw materials, simple preparation process, low cost, good freezing protection effect and stronger industrialized implementation.

The invention provides a method for enhancing workability of concrete. A technical scheme of the invention is that a workability improving agent with hydrophilic colloid as a main component is added into the concrete to enhance workability of the concrete. Adding the workability improving agent with hydrophilic colloid as the main component into the concrete can reduce sensitivity of water reducer to aggregate, improve concrete holding phenomenon during strong concrete pumping, reduce concrete pumping pressure, so as to endow better construction performance on the concrete.

The invention discloses a method for preparing blood glucose reducing peptides from flammulina velutipes. The method includes steps of mixing flammulina velutipes dry powder and sodium hydroxide aqueous solution with the mass concentration of 0.4%-0.6%, then carrying out water bath treatment, regulating a pH (potential of hydrogen) value of supernatant until the pH value of the supernatant reaches 4+ / -0.5, and then freezing and centrifuging the supernatant to obtain precipitates; adding water and neutral protease into the precipitates, carrying out enzymatic hydrolysis reaction under oscillation conditions, preserving heat of obtained enzymatic hydrolysis reaction products at the temperatures of 95-100 DEG C for 10-15 min, then naturally cooling the enzymatic hydrolysis reaction products until the temperatures of the enzymatic hydrolysis reaction products reach the room temperature and centrifuging the enzymatic hydrolysis reaction products to obtain enzymatic hydrolysate; carrying out ultra-filtration treatment on the enzymatic hydrolysate to obtain ultra-filtration enzymatic hydrolysate; carrying out spray drying on the ultra-filtration enzymatic hydrolysate to obtain blood glucose reducing peptide powder from the flammulina velutipes.

The present invention discloses a method of preparing antihypertensive peptide from silkworm pupa which includes the following steps: the defatted dry powder of silkworm pupa or silkworm pupa protein powder is mixed with water at the mass ratio of 1:6-7, the pH value is adjusted to 6.0-6.5, then neutral proteinase is added for hydrolysis at 50-55DEG C for 120-200 minutes, the result hydrolysate is antihypertensive peptide. The weight of the said neutral proteinase is 0.9-1.1% of the defatted dry powder of silkworm pupa or silkworm pupa protein powder. The method in the present invention that utilizes silkworm pupa to prepare antihypertensive peptide has lower cost and better effect.

The invention discloses a vacuum low-temperature continuous drying method for lactobacillus plantarum. The method comprises the following steps of (1) adding a drying protectant into a lactobacillus plantarum solution to prepare a uniform mixture; (2) feeding the uniform mixture obtained in the step (1) to a vacuum low-temperature continuous drying machine with the model of MJ103-6; and (3) carrying out vacuum storage on bacterial powder obtained in the step (2). The vacuum low-temperature continuous drying method can be used for effectively increasing the survival rate of lactobacillus in the drying process and realizing large-scale production and has relatively strong industrial feasibility.

The invention discloses a rapid preparation method of a mussel protein antihypertensive peptide. The antihypertensive peptide is characterized in that an amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp(VSWPCRW); the molecular weight determined by ESI-MS (electrospray ionization mass spectrometry) is 935.07Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp is obtained by shelling mussels, taking the meat of the mussels, carrying out enzymolysis on the meat, carrying out ultrafiltration on the enzymatic hydrolysate, adsorbing the enzymatic hydrolysate with an agarose magnetic microsphere immobilized ACE (angiotensin converting enzyme) and carrying out purification by RP-HPLC (reversed-phase high-performance liquid chromatography). The active peptide prepared by the invention has obvious ACE inhibitory activity and can be used in hypertension treatment related drugs.

The invention discloses a method for producing angiotensin converting enzyme inhibitory peptide with combined catalysis on hairtail chine, which includes the following procedures that: the hairctail chine is shattered and mixed evenly with water, with PH regulated and the temperature raised to 30-45 DEG C, added with elastase to undergo enzymatic hydrolysis, then the temperature of the hydrolysate liquid is raised rapidly to 90-100 DEG C and kept for 10-15 minutes, then cooled down rapidly to the room temperature to terminate the reaction, then the pH of the hydrolysate liquid is regualated to 1.5-3.0, which is added with pepsin for enzymatic hydrolysis, then the temperature of the reaction system is rapidly raised to 90-100DEG C, kept for 10-15 minutes, and cooled down to the room temperature rapidly to terminate the reaction, the hydrolysate liquid is centrifuged, and the supernate is harvested, condensed in vacuum, refrigerated and dried, to obtain the power-like angiotensin converting enzyme inhibitory peptide. The raw material of the invention is rich in raw material but low in price, and the technical transformation provided by the invention can improve the appendix value of hairtail chine as the residue material of processed marine products, and has importance to go on enhancing the development of the industry of processing of marine products.

The invention discloses high-concentration compound microorganism bacterial fertilizer and a production method thereof, wherein the production method comprises the following steps: respectively cultivating azotobacter chroococcum, bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis and bacillus macerans to obtain seeds for fermentation; sterilizing a production culture medium in a fermentation tank at the temperature of 121 DEG C for 20 minutes, inoculating strain seeds when the temperature of the culture medium decreases to about 33 DEG C, fermenting for 24 to 30 hours at the temperature of 33+ / -2 DEG C under the condition of introducing oxygen to obtain the compound microorganism bacterial fertilizer, wherein the number of effective live bacteria is larger than or equalto 5*109 (50 hundred million) / mL, and the number of each kind of bacteria is not lower than 3 hundred million / mL. The method adopted by the invention is scientific and reasonable, and has simple and easy operation steps, strong industrialized feasibility and important meanings for promoting agricultural production.

The invention discloses a method for improving the survival rate of lactobacilli after freeze drying by using prefreezing with liquid nitrogen, which comprises the following steps: (1) collecting well-cultivated bacterial sludge of lactobacilli and adding a protective agent according to a certain mixing ratio; (2) dripping the bacterial sludge into liquid nitrogen for prefreezing; (3) freeze drying the pre-frozen bacterial sludge; and (4) collecting product for storage under vacuum. The method of the invention effectively increases the number of live bacteria after freeze drying by replacing the conventional prefreezing method with prefreezing with the liquid nitrogen, and adopting the certain compound protective agent at the same time, is simple in related materials and convenient operation, and has high industrial implementation performance.