Method for preparing hypoglycemic peptide from silkworm chrysalis

A technology for lowering blood sugar and silkworm chrysalis, which is applied in the field of bioengineering, can solve the problems of not involving the inhibitory activity of α-glucosidase in animals, unreported mechanism of lowering blood sugar, high cost of raw materials, etc., and achieve strong industrial implementation, low price and easy operation simple effect

Active Publication Date: 2016-08-24
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the raw material cost of these methods is relatively high
[0004] In previous studies, Zhang Haina found that silkworm chrysalis protein prepared ACE activity inhibitory peptides with alkaline protease and complex protease, and at the same time had the effect of lowering blood sugar in mice, but the activity was not high, and its blood sugar lowering mechanism was not reported
Li Guorong hydrolyzed silkworm chrysalis powder with alkaline protease and compound protease to inhibit the activity of yeast α-glucosidase, but it did not involve the inhibitory activity of animal α-glucosidase, and the activity was not high
There is no report on the inhibitory activity of active peptides derived from silkworm chrysalis against α-glucosidase in animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, a kind of method utilizing silkworm chrysalis to prepare hypoglycemic peptide, carries out the following steps successively:

[0034] 1) Weigh 10 kg of pulverized degreased dry silkworm chrysalis powder, add 150 kg of water and stir evenly, adjust the pH value to 4.0 with an appropriate amount of HCl, and use it as a reaction starting material. Add 300 g of acid protease (enzyme activity: 50,000 U / g) to the medium, continue to keep warm (35°C-40°C) and stir continuously to carry out enzymolysis reaction for 4h.

[0035] After the reaction time is up, heat the product obtained from the enzymatic hydrolysis reaction to 95-100°C within 5-10 minutes, and keep it warm (95-100°C) for 10 minutes; then cool naturally to room temperature to terminate the reaction.

[0036] Centrifuge 20min under 4000r / min then, obtain supernatant 98kg, this supernatant is enzymolysis solution;

[0037] 2) Use 9.8 kg of decolorized activated carbon to decolorize the enzymatic hydro...

Embodiment 2

[0041] Embodiment 2, a kind of method utilizing silkworm chrysalis to prepare hypoglycemic peptide, carries out the following steps successively:

[0042] 1) Weigh 40 kg of pulverized degreased dry silkworm chrysalis powder, add 600 kg of water and stir evenly, adjust the pH value to 4.0 with an appropriate amount of HCl, and use it as a reaction starting material. Control the temperature at 35°C to 40°C. Add 800 g of acid protease (enzyme activity: 50,000 U / g) to the mixture, continue to incubate and stir continuously for enzymolysis reaction for 4 hours.

[0043] After the enzymolysis time is up, heat the product obtained from the enzymolysis reaction to 95-100°C within 5 to 10 minutes, and keep it warm for 10 minutes; then cool naturally to room temperature to terminate the reaction.

[0044] Centrifuge 20min under 4000r / min then, obtain supernatant 390kg, and this supernatant is enzymolysis solution;

[0045] 2) Use 39 kg of decolorizing activated carbon to decolorize the...

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PUM

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Abstract

The invention discloses a method for preparing hpyerglycemic peptide by using silkworm chrysalis. The method comprises the following steps: 1) adding water into degreased dried silkworm chrysalis powder, carrying out uniform mixing through stirring, then adjusting a pH value to 3.8 to 4.2, using the obtained mixture as a reaction starting substance, adding acidic protease into the reaction starting substance at 35 to 40 DEG C, carrying out a heat-preserved enzymatic hydrolysis reaction for 3.5 to 4.5 h under stirring and carrying out centrifugation after termination of the reaction so as to obtain a supernatant which is an enzymatic hydrolysis liquid; and 2) decoloring the enzymatic hydrolysis liquid with decoloring active carbon at 35 to 40 DEG C, subjecting the decolored enzymatic hydrolysis liquid to filtering so as to obtain a roughly filtered enzymatic hydrolysis liquid, carrying out ultrafiltration on the roughly filtered enzymatic hydrolysis liquid by using a hollow fiber polysulfone ultrafiltration membrane with a molecular weight cutoff of 3 Kda, concentrating the obtained ultrafiltered enzymatic hydrolysis liquid by using a vacuum thickener to a weight 25 to 40% of the original weight of the ultrafiltered enzymatic hydrolysis liquid and carrying out vacuum freeze drying so as to obtain peptide powder with a hpyerglycemic function.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing hypoglycemic peptides by using silkworm chrysalis. Background technique [0002] At present, diabetes has become a chronic disease that seriously threatens human health after tumors and cardiovascular diseases. It is one of the three major chronic diseases that threaten human health. Postprandial hyperglycemia is the main risk factor for diabetes. By inhibiting the activity of α-glucosidase on the brush border of the epithelial chorion of the small intestine, it reduces the digestion of carbohydrates in food and delays the production of glucose, which plays an important role in reducing postprandial blood sugar. Some chemically synthesized α-glucosidase inhibitors (such as acarbose tablets) have been developed and applied in clinical treatment. However, chemically synthesized α-glucosidase inhibitors can produce certain side effects on t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 张玉王伟王君虹
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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