Method for preparing hypoglycemic peptide from silkworm chrysalis

Abstract

The invention discloses a method for preparing hpyerglycemic peptide by using silkworm chrysalis. The method comprises the following steps: 1) adding water into degreased dried silkworm chrysalis powder, carrying out uniform mixing through stirring, then adjusting a pH value to 3.8 to 4.2, using the obtained mixture as a reaction starting substance, adding acidic protease into the reaction starting substance at 35 to 40 DEG C, carrying out a heat-preserved enzymatic hydrolysis reaction for 3.5 to 4.5 h under stirring and carrying out centrifugation after termination of the reaction so as to obtain a supernatant which is an enzymatic hydrolysis liquid; and 2) decoloring the enzymatic hydrolysis liquid with decoloring active carbon at 35 to 40 DEG C, subjecting the decolored enzymatic hydrolysis liquid to filtering so as to obtain a roughly filtered enzymatic hydrolysis liquid, carrying out ultrafiltration on the roughly filtered enzymatic hydrolysis liquid by using a hollow fiber polysulfone ultrafiltration membrane with a molecular weight cutoff of 3 Kda, concentrating the obtained ultrafiltered enzymatic hydrolysis liquid by using a vacuum thickener to a weight 25 to 40% of the original weight of the ultrafiltered enzymatic hydrolysis liquid and carrying out vacuum freeze drying so as to obtain peptide powder with a hpyerglycemic function.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing hypoglycemic peptides by using silkworm chrysalis. Background technique [0002] At present, diabetes has become a chronic disease that seriously threatens human health after tumors and cardiovascular diseases. It is one of the three major chronic diseases that threaten human health. Postprandial hyperglycemia is the main risk factor for diabetes. By inhibiting the activity of α-glucosidase on the brush border of the epithelial chorion of the small intestine, it reduces the digestion of carbohydrates in food and delays the production of glucose, which plays an important role in reducing postprandial blood sugar. Some chemically synthesized α-glucosidase inhibitors (such as acarbose tablets) have been developed and applied in clinical treatment. However, chemically synthesized α-glucosidase inhibitors can produce certain side effects on t...

AI Technical Summary

Problems solved by technology

However, the raw material cost of these methods is relatively high

[0004] In previous studies, Zhang Haina found that silkworm chrysalis protein prepared ACE activity inhibitory peptides with alkaline protease and complex protease, and at the same time had the effect of lowering blood sugar in mice, but the activity was not high, and its blood sugar lowering mechanism was not reported

Li Guorong hydrolyzed silkworm chrysalis powder with alkaline protease and compound protease to inhibit the activity of yeast α-glucosidase, but it did not involve the inhibitory activity of animal α-glucosidase, and the activity was not high

There is no report on the inhibitory activity of active peptides derived from silkworm chrysalis against α-glucosidase in animals