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Method for preparing hypoglycemic peptide by using Flammulina velutipes

A technology of Flammulina velutipes and hypoglycemia, which is applied in the field of bioengineering, can solve the problems of lack of high value-added products, backward processing technology of Flammulina velutipes, unfavorable sustainable development of Flammulina velutipes, etc., to achieve increased utilization value, strong industrial implementation, and strong inhibition of a- Effect of glucosidase activity

Active Publication Date: 2020-09-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] my country is a big country in the production and consumption of Flammulina velutipes. With the popularization of industrial production of Flammulina velutipes, the output of Flammulina velutipes has increased, but the deep processing technology of Flammulina velutipes is backward, and there is a lack of high value-added products, which is not conducive to the healthy and sustainable development of Flammulina velutipes industry

Method used

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  • Method for preparing hypoglycemic peptide by using Flammulina velutipes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of method utilizing Flammulina velutipes to prepare hypoglycemic peptide, carries out following steps successively:

[0030] 1), Weigh 2kg of Flammulina velutipes dry powder, add 24kg of 0.5% sodium hydroxide aqueous solution to mix, extract at 60°C for 2h, take the supernatant, adjust the pH value of the supernatant to 4.0 with 1moL / L HCl, 8000r / min Freeze (4°C) and centrifuge for 15 minutes to obtain 0.5kg of precipitate,

[0031] 2), add 1.5kg of water to the precipitate obtained in step 1) and stir evenly, use 0.1mol / L sodium hydroxide to adjust the pH to 7.0, heat up to 45°C, then add 5.0g of neutral protease (100U / mg), Enzymolysis reaction at 45°C for 3 hours under shaking conditions (180r / min);

[0032] 3), heat up the enzymolysis reaction product obtained in step 2) to 95-100° C. within 10 minutes, maintain this temperature for 15 minutes, and cool it down to room temperature naturally. Centrifuge at 4°C for 20 minutes, and the supernatant...

Embodiment 2

[0043] Embodiment 2, a kind of method utilizing Flammulina velutipes to prepare hypoglycemic peptide, carries out following steps successively:

[0044] 1), Weigh 5kg of Flammulina velutipes dry powder, add 60kg of 0.5% sodium hydroxide aqueous solution to mix, extract at 60°C for 2h, take the supernatant, adjust the pH value of the supernatant to 4.0 with 1moL / L HCl, 8000r / min Freeze (4°C) and centrifuge for 15 minutes to obtain 1.3kg of precipitate;

[0045] 2), add 6.5kg of water to the precipitate obtained in step 1) and stir evenly, use 0.1mol / L sodium hydroxide to adjust the pH value to 7.5, heat up to 50°C, then add 13.0g of neutral protease (100U / mg), Enzymolysis reaction at 50°C for 4 hours under shaking conditions (180r / min);

[0046] Subsequent steps are the same as in Example 1.

[0047] The obtained Flammulina velutipes hypoglycemic peptide powder is slightly yellow in color. Configured as a 3mg / mL solution, the measured inhibitory activity on a-glucosidase was 6...

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Abstract

The invention discloses a method for preparing blood glucose reducing peptides from flammulina velutipes. The method includes steps of mixing flammulina velutipes dry powder and sodium hydroxide aqueous solution with the mass concentration of 0.4%-0.6%, then carrying out water bath treatment, regulating a pH (potential of hydrogen) value of supernatant until the pH value of the supernatant reaches 4+ / -0.5, and then freezing and centrifuging the supernatant to obtain precipitates; adding water and neutral protease into the precipitates, carrying out enzymatic hydrolysis reaction under oscillation conditions, preserving heat of obtained enzymatic hydrolysis reaction products at the temperatures of 95-100 DEG C for 10-15 min, then naturally cooling the enzymatic hydrolysis reaction products until the temperatures of the enzymatic hydrolysis reaction products reach the room temperature and centrifuging the enzymatic hydrolysis reaction products to obtain enzymatic hydrolysate; carrying out ultra-filtration treatment on the enzymatic hydrolysate to obtain ultra-filtration enzymatic hydrolysate; carrying out spray drying on the ultra-filtration enzymatic hydrolysate to obtain blood glucose reducing peptide powder from the flammulina velutipes.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing hypoglycemic peptides from Flammulina velutipes. Background technique [0002] At present, diabetes has become a chronic disease that seriously threatens human health after tumors and cardiovascular diseases. It is one of the three major chronic diseases that threaten human health. Postprandial hyperglycemia is the main risk factor for diabetes. By inhibiting the activity of α-glucosidase on the brush border of the epithelial chorion of the small intestine, it reduces the digestion of carbohydrates in food and delays the production of glucose, which plays an important role in reducing postprandial blood sugar. The α-glucosidase inhibitors currently used in clinical treatment are mostly chemically synthesized, and long-term use can cause certain side effects on the human body, such as damage to the liver, leading to flatulence and intestin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A61K38/01A61P3/10
CPCA23L33/18A23V2002/00A61K38/005A61K38/011C07K1/34C12P21/06A23V2200/328A23V2250/55
Inventor 张玉王伟王君虹朱作艺李雪
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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