Rapid preparation method of mussel protein antihypertensive peptide

A technology of antihypertensive peptides and mussels, which is applied in the field of bioengineering, can solve the problems of preparing antihypertensive peptides, and achieve the effects of improving utilization, significant ACE inhibitory activity, and simple operation

Active Publication Date: 2020-08-11
山东大树达孚特膳食品有限公司
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  • Abstract
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Problems solved by technology

However, the researchers found that there is no research on the preparation of antihypertensive peptides from mussel hydrolyzate by using this technology

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  • Rapid preparation method of mussel protein antihypertensive peptide

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Embodiment

[0024] A rapid preparation method of mussel protein antihypertensive peptide, the preparation process is as follows: mussel meat "tissue fragmentation" enzymatic hydrolysis, "ultrafiltration" agarose magnetic microspheres immobilized ACE adsorption, "RP-HPLC purification" antihypertensive peptide.

[0025] ) Preparation and activation of agarose magnetic microspheres: add agarose to water according to the weight-volume ratio of 1.5g: 30 mL, boil together until the agarose is completely dissolved, add 1 ml Fe 3 O 4 Magnetic fluid, fully mixed on a vortex mixer; drip the mixed liquid into the organic phase (Span-80: liquid paraffin = 1g: 25mL) at 80°C under stirring, and react at 80°C for 20 minutes; the organic phase is cooled Stir mechanically at 250 rpm for 15 min at 40°C to form microspheres and separate them in a magnetic field. The prepared microspheres were washed 3 times with petroleum ether and deionized water to remove residual organic phases to obtain agarose magnetic micr...

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Abstract

The invention discloses a rapid preparation method of a mussel protein antihypertensive peptide. The antihypertensive peptide is characterized in that an amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp(VSWPCRW); the molecular weight determined by ESI-MS (electrospray ionization mass spectrometry) is 935.07Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp is obtained by shelling mussels, taking the meat of the mussels, carrying out enzymolysis on the meat, carrying out ultrafiltration on the enzymatic hydrolysate, adsorbing the enzymatic hydrolysate with an agarose magnetic microsphere immobilized ACE (angiotensin converting enzyme) and carrying out purification by RP-HPLC (reversed-phase high-performance liquid chromatography). The active peptide prepared by the invention has obvious ACE inhibitory activity and can be used in hypertension treatment related drugs.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a rapid preparation method of mussel protein antihypertensive peptide. Background technique [0002] Angiotensin-converting enzyme (ACE) is also called peptidyl-carboxypeptidase or kininase II, with a molecular weight of 150,000 Da, and is a membrane-bound enzyme of vascular endothelial cells. ACE can hydrolyze the angiotensin I of the decapeptide into angiotensin II of the octapeptide, which promotes the further contraction of blood vessels, leading to an increase in blood pressure. At the same time, ACE can also promote the secretion of aldosterone by acting on the adrenal cortex. Therefore, ACE is an important component of renin-angiotensin-aldosterone (RAS). In addition, ACE can also catalyze the hydrolysis of bradykinin that has a hypotensive effect and make it inactive. Therefore, looking for suitable substances that inhibit the activity of ACE in the body c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/22C07K1/20A61P9/12B01J13/02B01J20/24B01J20/30B01J20/28
Inventor 王斌迟长凤赵玉勤孙坤来
Owner 山东大树达孚特膳食品有限公司
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