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Mussel Protein Antihypertensive Peptide

A technology for antihypertensive peptides and proteins, applied in the field of bioengineering, can solve problems such as preparing antihypertensive peptides, and achieve the effects of improving utilization, scientific and reasonable process, and strong industrial implementation.

Active Publication Date: 2020-09-08
安徽盛美诺生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the researchers found that there is no research on the preparation of antihypertensive peptides from mussel hydrolyzate by using this technology

Method used

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  • Mussel Protein Antihypertensive Peptide

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Embodiment

[0023] A rapid preparation method of mussel protein antihypertensive peptide, the preparation process is as follows: mussel meat "tissue fragmentation" enzymatic hydrolysis, "ultrafiltration" agarose magnetic microspheres immobilized ACE adsorption, "RP-HPLC purification" antihypertensive peptide.

[0024] 1) Preparation and activation of agarose magnetic microspheres: add agarose to water according to the weight-volume ratio of 1.5g:30mL, boil together until the agarose is completely dissolved, add 1ml Fe 3 O 4 Magnetic fluid, fully mixed on a vortex mixer; drip the mixed liquid into the organic phase (Span-80: liquid paraffin = 1g: 25mL) at 80°C under stirring, and react at 80°C for 20 minutes; the organic phase is cooled Stir mechanically at 250 rpm for 15 min at 40°C to form microspheres and separate them in a magnetic field. The prepared microspheres were washed 3 times with petroleum ether and deionized water to remove the residual organic phase to obtain agarose magnetic mic...

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Abstract

The invention discloses a mussel protein antihypertensive peptide with an antihypertensive activity as well as a preparation method and an application thereof. The amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW), and the molecular weight measured by ESI-MS is 935.07 Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW) is obtained through shelling and meat taking, enzymolysis, ultrafiltration, agarose magnetic microsphere immobilized ACE adsorption and reversed-phase high-performance liquid chromatography (RP-HPLC) purification. The active peptide prepared by the preparation method disclosed by the invention is remarkable in angiotensin converting enzyme (ACE) inhibitory activity, and capable of being used for hypertension treatment-related medicines.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a mussel protein antihypertensive peptide. Background technique [0002] Angiotensin-converting enzyme (ACE) is also called peptidyl-carboxypeptidase or kininase II, with a molecular weight of 150,000 Da, and is a vascular endothelial cell membrane-bound enzyme. ACE can hydrolyze the angiotensin I of the decapeptide into angiotensin II of the octapeptide, which promotes the further contraction of blood vessels, leading to an increase in blood pressure. At the same time, ACE can also promote the secretion of aldosterone by acting on the adrenal cortex. Therefore, ACE is an important component of renin-angiotensin-aldosterone (RAS). In addition, ACE can also catalyze the hydrolysis of bradykinin that has a hypotensive effect and make it inactive. Therefore, looking for suitable substances that inhibit ACE activity in the body can achieve the purpose of lowering or c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61P9/12
Inventor 王斌迟长凤赵玉勤孙坤来
Owner 安徽盛美诺生物技术有限公司
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