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Method for producing anti-hypertension peptide with complex zymohydrolysis of hairtail backbone

A blood pressure-lowering peptide and hairtail technology, applied in the field of bioengineering, can solve the problems of hairtail spine utilization and deep processing that are still blank, and achieve the effects of strong industrial implementation, simple operation, and shortened enzymatic hydrolysis time

Inactive Publication Date: 2011-09-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly used in the feed industry, and it is still blank for the utilization and deep processing of the hairtail backbone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take by weighing hairtail backbone (moisture 76.22%, protein content 12.17%, Zhejiang Province Ningbo Chaoxing Marine Biological Products Co., Ltd. provides) 20kg after the homogenization, add water 80kg and stir with adjusting pH value after 8.0, temperature is raised to 37 After keeping for 20 minutes, add 0.02kg of elastase 0.02kg (enzyme activity is 15000U / g) of 0.1% of hairtail backbone weight in the raw material, continue to incubate and constantly stir to carry out enzymolysis reaction for 3 hours. Then use the enzymolysis solution to rapidly heat up to 90-100° C., keep it for 10-15 minutes, and then rapidly cool down to room temperature to terminate the reaction. Adjust the pH of the enzymolysis solution to 2.0, keep the temperature at 37° C. for about 20 minutes, add 0.002 kg of pepsin with a content of 0.1% of the original protein, continue to keep warm and stir continuously to carry out the enzymolysis reaction for 2 hours. Then use the enzymolysis solution t...

Embodiment 2

[0025] Take by weighing hairtail backbone (moisture 76.22%, protein content 12.17%, Zhejiang Province Ningbo Chaoxing Marine Biological Products Co., Ltd. provides) 10kg after the homogenization, add water 30kg and stir with adjusting pH value after 7.0, temperature is raised to 37 ℃, add 0.012kg of elastase (enzyme activity is 15000U / g) of 0.12% of hairtail backbone weight in the raw material, continue to insulate and constantly stir to carry out enzymolysis reaction 2 hours. Then use the enzymolysis solution to rapidly heat up to 90-100° C., keep it for 10-15 minutes, and then rapidly cool down to room temperature to terminate the reaction. Adjust the pH of the enzymolysis solution to 2.0, keep the temperature at 37° C. for about 20 minutes, add 0.002 kg of pepsin with a content of 0.1% of the original protein, continue to keep warm and stir continuously to carry out the enzymolysis reaction for 1 hour. Then use the enzymolysis solution to rapidly heat up to 90-100° C., keep...

Embodiment 3

[0027]Take by weighing hairtail backbone (moisture 76.22%, protein content 12.17%, Zhejiang Province Ningbo Chaoxing Marine Biological Products Co., Ltd. provides) 25kg after the homogenization, add water 125kg and stir with adjusting pH value after 8.0, temperature is raised to 37 After keeping for 20 minutes, add 0.03kg of 0.12% elastase (enzyme activity is 15000U / g) of hairtail backbone weight in the raw material, continue to incubate and constantly stir to carry out enzymolysis reaction for 3 hours. Then use the enzymolysis solution to rapidly heat up to 90-100° C., keep it for 10-15 minutes, and then rapidly cool down to room temperature to terminate the reaction. Adjust the pH of the enzymolysis solution to 1.5, keep the temperature at 37° C. for about 20 minutes, add 0.0025 kg of pepsin with a content of 0.1% of the original protein, continue to keep warm and stir continuously to carry out the enzymolysis reaction for 1 hour. Then use the enzymolysis solution to rapidly...

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PUM

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Abstract

The invention discloses a method for producing angiotensin converting enzyme inhibitory peptide with combined catalysis on hairtail chine, which includes the following procedures that: the hairctail chine is shattered and mixed evenly with water, with PH regulated and the temperature raised to 30-45 DEG C, added with elastase to undergo enzymatic hydrolysis, then the temperature of the hydrolysate liquid is raised rapidly to 90-100 DEG C and kept for 10-15 minutes, then cooled down rapidly to the room temperature to terminate the reaction, then the pH of the hydrolysate liquid is regualated to 1.5-3.0, which is added with pepsin for enzymatic hydrolysis, then the temperature of the reaction system is rapidly raised to 90-100DEG C, kept for 10-15 minutes, and cooled down to the room temperature rapidly to terminate the reaction, the hydrolysate liquid is centrifuged, and the supernate is harvested, condensed in vacuum, refrigerated and dried, to obtain the power-like angiotensin converting enzyme inhibitory peptide. The raw material of the invention is rich in raw material but low in price, and the technical transformation provided by the invention can improve the appendix value of hairtail chine as the residue material of processed marine products, and has importance to go on enhancing the development of the industry of processing of marine products.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to biopharmaceutical technology and functional food production and processing technology. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in blood pressure regulation. It converts the inactive angiotensin decapeptide precursor to angiotensin, which increases blood pressure. Angiotensin-converting enzyme (ACE) inhibitory active peptide is a kind of angiotensin-converting enzyme inhibitor (ACEI), which can lower blood pressure by inhibiting the activity of ACE. There are many kinds of chemically synthesized peptide drugs as ACE inhibitors. The disadvantage of this kind of synthetic antihypertensive drugs is that the action time is not long, the blood pressure is easy to rebound, and the drugs are easily excreted by the kidneys to cause damage to renal function. [0003] Extracting functional peptides with nutritional and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A61K35/60A61P9/12
Inventor 王金玲何国庆章海峰阮晖陈启和王凤平
Owner ZHEJIANG UNIV
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