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Preparation of depressor peptide by silkworm chrysalis

A blood pressure-lowering peptide and silkworm chrysalis technology, applied in the field of bioengineering, can solve the problems such as the technology of blood pressure-lowering peptide has not been seen before, and achieve the effects of strong industrial implementation, increased added value and simple operation.

Inactive Publication Date: 2011-07-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few domestic reports on the preparation of antihypertensive peptides from food proteins, and the technology of preparing antihypertensive peptides by using degreased dried silkworm chrysalis after reeling has not been seen in the world.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take by weighing 50 kg of degreased dry silkworm chrysalis powder (moisture 1.2%, protein content 66.23%, purchased from Zhejiang Silk Factory No. 1) after pulverization, add 320 kg of water and stir evenly, and use HCl with a concentration of 2N to adjust the pH value to 6.0~6.5; then The temperature of the entire reaction system was raised to 50°C to 55°C, and kept warm for 20 minutes; then 0.5kg of neutral protease (enzyme activity: 1.5AU / g) was added, kept warm and kept stirring for 150 minutes of enzymolysis reaction. After the time of 150 minutes is up, the enzymolysis reaction product is rapidly heated to 85° C. and kept for 10-15 minutes; then rapidly cooled to room temperature (ie 0-30° C.) to terminate the enzymolysis reaction.

[0021] Centrifuge in J-6M large-capacity refrigerated centrifuge (BECKMAN company) at 3000r / min at 4°C for 20 minutes to obtain 220kg of supernatant, which is the enzymatic hydrolysis solution; the precipitate obtained by centrifugatio...

Embodiment 2

[0024] Take by weighing 10 kg of degreased dry silkworm chrysalis powder (moisture content 1.2%, protein content 66.23%, purchased from No. 1 Zhejiang Silk Factory) after pulverization, utilize 1.0% NaOH to extract protein, temperature 60 ℃, extraction time is 4 hours, in J-6M Type large-capacity refrigerated centrifuge (BECKMAN company) 3000r / min under the condition of 4 ℃ of centrifugation 20 minutes, supernatant is that the HCl of 2N pH value is reduced to 4.6, precipitates after centrifugal freeze-drying and obtains silkworm chrysalis protein powder 2.38kg, The protein content is 96.4%.

[0025] Take 2kg of the above silkworm chrysalis protein powder, add 12kg of water and stir well, then adjust the pH value to 6.0-6.5 with 2N HCl; Protease (enzyme activity is 1.5AU / g), continue to incubate and stir constantly to carry out enzymolysis reaction for 120-200 minutes. After the set reaction time is up, the enzymatic hydrolysis reaction product is rapidly heated to 85° C. and ...

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Abstract

The present invention discloses a method of preparing antihypertensive peptide from silkworm pupa which includes the following steps: the defatted dry powder of silkworm pupa or silkworm pupa protein powder is mixed with water at the mass ratio of 1:6-7, the pH value is adjusted to 6.0-6.5, then neutral proteinase is added for hydrolysis at 50-55DEG C for 120-200 minutes, the result hydrolysate is antihypertensive peptide. The weight of the said neutral proteinase is 0.9-1.1% of the defatted dry powder of silkworm pupa or silkworm pupa protein powder. The method in the present invention that utilizes silkworm pupa to prepare antihypertensive peptide has lower cost and better effect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing hypotensive peptides by using silkworm chrysalis. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in blood pressure regulation. Angiotensin-converting enzyme (ACE) inhibitory active peptide is a kind of angiotensin-converting enzyme inhibitor (ACEI), which can lower blood pressure by inhibiting the activity of ACE. There are many kinds of chemically synthesized peptide drugs as ACE inhibitors. The disadvantage of this kind of synthetic antihypertensive drugs is that the action time is not long, the blood pressure is easy to rebound, and the drugs are easily excreted by the kidneys to cause damage to renal function. [0003] Extracting functional peptides with nutritional and physiological activities from natural proteins, and applying these functional peptides to health food and fortified nutrit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02A61P9/12
Inventor 何国庆王伟陈启和
Owner ZHEJIANG UNIV
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