Method of utilizing duck blood to prepare antibacterial peptide

An antimicrobial peptide and duck blood technology, applied in the field of bioengineering, can solve the problems of antimicrobial peptide extraction in blood and few reports on antibacterial activity research, and achieve the effects of strong industrial implementation, increased added value, and simple operation

Inactive Publication Date: 2015-12-02
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic and foreign research on duck-derived antimicrobial peptides mainly focuses on antimicrobial peptides in duck small intestine, but there are few reports on the extraction and antibacterial activity of antimicrobial peptides in blood at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Collect 10L of fresh duck blood, anticoagulate with 3g / L sodium citrate, add 105L distilled water to the duck blood at 4°C, ultrasonically disrupt the cells to assist hemolysis for 20min, and then centrifuge at 4°C and 4000r / min After 10 minutes, 20 L of hemolysis solution was obtained.

[0016] (2) In the hemolysis solution, add 100g alkaline protease for enzymatic hydrolysis (the specific activity of the enzyme is 10×10 4 u / g), stirring, 1mol / L NaoH solution to maintain the pH of the system at 8.0, 50°C, 5h; the enzymolysis reaction product was quickly heated to 85°C and kept at this temperature for 15 minutes, and then rapidly cooled to 0-30°C to terminate the enzyme hydrolysis reaction, adjust the pH value to about 7.0, centrifuge at 8000r / min for 15min, and obtain 19L of supernatant enzymatic hydrolysis solution.

[0017] (3) Add 3kg of activated carbon to the enzymatic hydrolysis solution, stir at 55°C for 20-30 minutes to decolorize; then perform coarse filt...

Embodiment 2

[0020] (1) Collect 50L of fresh duck blood, anticoagulate with 3g / L sodium citrate, add 50L of distilled water to duck blood at 4°C, ultrasonically disrupt cells to assist hemolysis for 20min, and then centrifuge at 4°C and 4000r / min for 10min , to obtain 100 L of hemolysis solution.

[0021] (2) In the hemolysis solution, add 500g of alkaline protease for enzymatic hydrolysis (the specific activity of the enzyme is 10×10 4 u / g), stirring, 1mol / L NaoH solution to maintain the pH of the system at 8.0, 50°C, 5h; the enzymolysis reaction product was quickly heated to 85°C and kept at this temperature for 15 minutes, and then rapidly cooled to 0-30°C to terminate the enzyme For hydrolysis reaction, adjust the pH value to about 7.0, centrifuge at 8000r / min for 15min, and obtain 100L of supernatant enzymatic hydrolysis solution.

[0022] (3) Add 15kg of activated carbon to the enzymatic hydrolysis solution, stir at 55°C for 20-30 minutes to decolorize; then perform coarse filtratio...

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PUM

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Abstract

The invention discloses a method of utilizing duck blood to prepare antibacterial peptide. The method includes the following steps: hemolyzing duck blood; enzymolyzing; decoloring; performing ultrafiltration; performing vacuum concentration; freeze-drying to obtain antibacterial peptide. Adding quantity of alkaline protease is 5000U / g, and enzymolysis reaction time is 4-6h with pH to be 8 and at temperature of 45-55 DEG C. The method is low in cost and good in effect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing antibacterial peptides from duck blood. Background technique [0002] Antibacterial peptides (antibacterialpeptide, ABP) is an important part of the natural immune defense system of organisms. Antimicrobial peptides have many advantages such as broad antibacterial spectrum, high thermal stability, safety and high efficiency. The preparation of food-source protein antimicrobial peptides has become one of the research hotspots in the field of biology. The domestic and foreign studies on duck-derived antimicrobial peptides mainly focus on the antimicrobial peptides in duck small intestine, but there are few reports on the extraction and antibacterial activity of antimicrobial peptides in blood at home and abroad. At present, the amount of ducks raised in our country is very large, and the leftovers of slaughtered ducks are a lot of duck blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 邓小玲宁发子殷华
Owner CHONGQING DOUHAO BIOTECH
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