4results about How to "Ensure application security" patented technology

The invention relates to the technical field of functional compositions, in particular to an anti-wrinkle and firming functional composition containing glassine as well as a preparation method and application of the anti-wrinkle and firming functional composition containing glassine. The composition containing the botryine and having the anti-wrinkle and firming effects is prepared from the following components: 3 to 15 parts of an RS configuration botryine solution, 1 to 3 parts of a spherical PDRN compound, 0.1 to 0.5 part of a folium achilleae millefolium extract wrapped by maltodextrin and 0.5 to 1.25 parts of a sodium mannose phosphate solution, the RS-configuration glassine solution comprises R-type glassine and S-type glassine in a mass ratio of (7-8): (2-3); the spherical PDRN compound is prepared from poly (deoxyribonucleotide) (PDRN), hydrolyzed sodium hyaluronate, 1, 2-pentanediol, 1, 2-hexanediol and deionized water; the sodium mannose phosphate solution comprises sodium mannose phosphate, glycerol and water. Through the multi-target multi-layer synergistic effect of all the components, the obvious tightening and wrinkle fading effects superior to those of a single component or a conventional compound are obtained.

The application provides a self-adaptive large model prompt attack and security evaluation method based on feedback learning, and belongs to the technical field of artificial intelligence security. The method comprises the following steps: constructing an attack library containing multiple text transformation attack strategies, and obtaining performance data of the attack strategies on a target model; then, the success rate, average score, R value and Q value of the attack strategies are calculated, and the optimal attack strategy is selected in descending order of the Q value; the strategy is applied to generate an adversarial input from an original malicious prompt, and the attack is judged to be successful or not by a pre-training evaluation model score after the adversarial input is submitted to the target model; if the attack is not successful, the strategy is reselected; if the attack is successful, the statistical data are updated and the score is recalculated; the strategy is iteratively tried according to the Q value until a preset condition is met; finally, the test process is recorded and a report is generated. The method realizes the security evaluation of the large model, improves the success rate and efficiency of the attack test, provides empirical evidence for the design of the large model defense mechanism, and guarantees the application safety of the large model.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The invention belongs to the technical field of biological product preparation, and particularly relates to a method for preparing a platelet lysate through combination of freeze thawing and ultrasound and application of the platelet lysate. The method provided by the invention comprises the following steps: S1, preparing and obtaining platelet-rich plasma (PRP) by taking umbilical cord blood as a raw material; s2, adding a protective agent into the platelet-rich plasma prepared in the step S1; and S3, preparing platelet lysate (HPL) by using a freeze thawing and ultrasonic combination method. The platelet membrane can be more easily cracked by freezing and thawing through a combined method and then carrying out ultrasonic treatment; according to the protective agent, glutathione and glycerol are added to inhibit active oxygen generated by destroying cell membranes due to the cavitation effect in the ultrasonic process and protein structure damage caused by ice crystal formation in the freezing and thawing process, so that the problems of incomplete platelet lysis, serious growth factor and protein loss and the like in the HPL preparation process are effectively solved, the operation is simple and convenient, and the cost is low. And the cost can be effectively saved.