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A kind of purification method of hazelnut shell brown pigment

A brown and brown pigment technology of hazelnut shell, applied in the field of bioengineering, can solve the problems of blank purification process of brown pigment of hazelnut shell, membrane pollution, natural pigment pollution, etc., and achieve strong industrial implementation, scientific and reasonable process, and good solubility Effect

Inactive Publication Date: 2016-03-30
哈尔滨丹彤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the gel is used many times, the gel particles may gradually deposit and compact, the flow rate will slow down, and the work efficiency will decrease, which is not conducive to industrial production
[0005] (2) Enzymatic method: The impurities in the crude product of natural pigments are removed by enzymatic reaction. This method has the characteristics of specificity and needs to be combined with other methods to achieve better results, thus limiting its use in industrial production. usage of
[0006] (3) Membrane separation method: Membrane separation technology is an emerging high-efficiency separation technology. Although the method is simple to operate, the membrane is easily polluted by microorganisms during the operation, thereby polluting the separated natural pigments and requires cleaning. Regular cleaning of the membrane with the cleaning agent will cause the residue of the cleaning agent on the membrane, which also affects the safety of natural pigments to a certain extent.
At present, the methods for extracting brown pigment from hazelnut shell mainly include alkali extraction method and microwave-assisted method, but the purification process of brown pigment from hazelnut shell is still blank. A simple and easy method for the enrichment and purification of brown pigment from hazelnut shell is established. , it is particularly important to the deep development of hazelnut shells, and the present invention provides a method for purifying brown pigment from hazelnut shells with macroporous resins just based on this

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0034] According to the method for preparing the brown pigment crude extract of hazelnut shells published by the patent number CN101798468A, the brown pigment crude extract of hazelnut shells was prepared: 10 kg of crushed hazelnut shells were weighed, 25 kg of a 0.5% NaCl aqueous solution was added, and the temperature was raised to 30 ℃, after keeping for 80 minutes, add 50 kg of NaOH aqueous solution with a mass fraction of 4%, raise the temperature to 50 ℃, continue to keep warm and stir constantly for extraction for 3 hours. Then the extract is cooled to room temperature, adding hydrochloric acid aqueous solution to adjust the pH to be about 7.5, centrifugal 30min under the condition of J-6M large-capacity refrigerated centrifuge (BECKMAN company) 3000rpm, supernatant is concentrated to 10kg, adds 40kg dehydrated alcohol, again Centrifuge to obtain 44.6 kg of supernatant, concentrate, and freeze-dry to obtain 0.96 kg of brown pigment crude extract of brown hazelnut shell. ...

preparation example 2

[0036]Preparation of a separation column equipped with macroporous resin: select a separation column with a volume of 250 mL, measure 200 mL of macroporous resin in a beaker, soak it in 600 mL of absolute ethanol for 24 hours, and place the soaked macroporous resin in a 200 Mesh filter funnel, rinse with 10L distilled water; then soak with 600mL of 4% sodium hydroxide aqueous solution for 24h, put the soaked macroporous resin in a funnel with 200 mesh filter, wash with 18L distilled water Rinse; finally use 600mL of 4% hydrochloric acid aqueous solution to soak for 24h, place the soaked macroporous resin in a funnel with a 200-mesh filter, and rinse with 18L of distilled water; wet the pretreated macroporous resin 10 separation columns equipped with macroporous resin were prepared in the same way and set aside.

Embodiment 1

[0038] Weigh 50.0 g of the hazelnut shell brown pigment crude extract of hazelnut shell brown pigment described in Preparation Example 1, add water to 2000 mL, stir to dissolve, and adjust its pH to 7 with a 4% hydrochloric acid aqueous solution to obtain hazelnut shell brown pigment Crude extract solution; 620mL hazelnut shell brown pigment crude extract solution is passed through the separation column equipped with macroporous resin prepared in Preparation Example 2 at a flow rate of 2.0mL / min, and the hazelnut shell brown pigment crude extract solution is all passed through the separation column After that, let it stand for adsorption for 70min, then rinse with 1200mL distilled water at a flow rate of 1.5mL / min until the effluent is colorless; The macroporous resin of the shell brown pigment crude extract, when the effluent is brown, collect the eluate, and stop collecting when the effluent turns yellow. The collected eluent was concentrated by rotary evaporation at a tempe...

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Abstract

The invention discloses a method for purifying a hazelnut shell brown pigment. A D101 type macroporous resin is employed for secondary purification on a crude extract of the hazelnut shell brown pigment, and the method includes resin adsorption, distilled water leaching, ethanol desorption, concentration and drying, so as to obtain purified hazelnut shell brown pigment. The macroporous resin method for purification of hazelnut shell brown pigment provided by the invention has the advantages of simple operation, safety and no toxicity, provides technical support for the development and utilization of the hazelnut shell brown pigment and provides a high quality natural raw material for the development of functional food and food additives, and truly realizes safe, simple, reasonable, economical and high value utilization of agricultural and forestry resources and industrialized production of series products.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a method for separating and purifying natural pigments, in particular to a method for separating and purifying brown pigment from hazelnut shells with a macroporous resin. Background technique [0002] In recent years, with the continuous advancement of science and technology, people have discovered that many varieties of synthetic pigments have chronic toxicity and carcinogenicity. Therefore, the variety and quantity of synthetic pigments have gradually decreased. In the middle of the 20th century, there were more than 100 kinds of chemically synthesized pigments in the world, and now there are only more than 60 kinds; Japan once approved the use of 27 kinds of chemically synthesized pigments, which has been reduced to 9 kinds; the United States allowed the use of 35 kinds at that time, and now only 7 kinds species; in addition, some countries, such as Norway, have completel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54
Inventor 姚丽敏王金玲
Owner 哈尔滨丹彤生物科技有限公司
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