Method for preparing antihypertensive peptide by using catfish bone

A technology of hypotensive peptide and catfish, which is applied in the field of preparation of hypotensive peptide, can solve the problems of environmental pollution, less comprehensive processing and utilization, waste, etc., and achieve the effects of reducing pollution, strong industrial implementation, and no drug resistance

Inactive Publication Date: 2015-11-18
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is rich in freshwater fish resources, and its output ranks first in the world. In the process of fish processing, fish bones are often discarded as leftovers, and less comprehensively processed and utilized, resulting in a lot of waste and easy pollution to the environment.

Method used

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Embodiment Construction

[0014] A kind of method utilizing catfish bone to prepare hypotensive peptide is characterized in that it comprises the following steps:

[0015] (1) Take fresh catfish bones, wash them, cook them under high pressure for 4 hours, add distilled water at a mass volume ratio of 1:1, pulverize them and make catfish bone paste;

[0016] (2) Take a certain amount of catfish bone slime 50kg, dissolve it in 100L deionized water, stir well, heat to 90°C for 10 minutes, cool to 60°C; adjust pH to 8.5, add 65g alkaline protease (enzyme specific activity 20× 10 4 u / g), enzymolysis at 55℃~60℃ for 6h, the temperature of the product of the enzymolysis reaction was raised to 90℃ rapidly and kept at this temperature for 10 minutes, then rapidly cooled to 0~30℃ to terminate the enzymolysis reaction, and the pH value was adjusted to 7.0 Centrifuge at 8000r / min for 15min to obtain supernatant enzymatic hydrolysis;

[0017] (3) The amount of activated carbon is 10k of the total weight of the enz...

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PUM

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Abstract

The invention discloses a method for preparing antihypertensive peptide by using catfish bone. The method comprises the following steps: cleaning the catfish bone, stewing the catfish bone to obtain catfish bone paste, carrying out enzymolysis, decoloring, carrying out ultrafiltration and vacuum concentration, and freeze-drying so as to obtain antihypertensive peptide. According to the method, the enzyme dosage of alkaline protease is that the enzyme-substrate ratio is 500 U / g, and the enzymatic reaction time is 5-7 h under the conditions that pH is 8.5 and the temperature is 55-60 DEG C. The molecular weight of antihypertensive peptide prepared through the method provided by the invention is smaller than 5 kD, and the ACE inhibition rate of antihypertensive peptide reaches 88% or above and is higher than those of antihypertensive drugs nimodipine and nifedipine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing blood pressure-lowering peptides from catfish bones. Background technique [0002] Hypertension is a cardiovascular disease that plagues the world today, and it is also the most important factor inducing coronary heart disease and heart and kidney failure, so it is called the "silent killer". Angiotensin-converting enzyme (ACE) plays an important role in the formation of blood pressure in humans. Since Ferreirra first isolated ACE inhibitory peptides from Agkistrodon venom in 1965, people have found ACE inhibitory peptides from many food proteins, such as cow milk casein, yogurt, garlic, soybeans, etc., but isolated ACE inhibitory peptides from bone research is still less. my country is rich in freshwater fish resources, and its output ranks first in the world. In the process of fish processing, fish bones are often discarded as leftovers, and sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 邓小玲宁发子宁成勇
Owner CHONGQING DOUHAO BIOTECH
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