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Method for preparing blood fat reducing peptide by use of silkworm chrysalis
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A technology for reducing blood fat and silkworm chrysalis, which is applied in the field of bioengineering, can solve the problems of poor blood fat lowering effect, and achieve the effect of low price, strong industrial implementation, and wide sources
Active Publication Date: 2015-05-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology
[0008] What can be obtained by this method is the hypotensive peptide, which has a relatively poor effect on reducing blood lipids
Method used
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Embodiment 1
[0031] Embodiment 1, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out following steps successively:
[0032] 1) Weigh 50kg of pulverized degreased dry silkworm chrysalis powder (moisture content 1.5%, protein content 75.03%, raw material comes from Nantong Fulier Biological Products Co., Ltd.), add 500kg water and stir well, adjust the pH value to 7.0 (use The concentration is 2mol / L HCl solution, NaOH solution to adjust, this adjustment method belongs to the conventional technology), first raise the temperature to 90°C within 5~10min and keep this temperature for 10min, then naturally cool to 45°C~50°C and keep it warm, as the starting point of the reaction starting material; add 1 kg of papain (enzyme activity: 800AU / g) to the reaction starting material, continue to incubate and stir continuously for enzymolysis reaction for 3 hours.
[0033] After the time is up, heat the product obtained from the enzymatic hydrolysis reaction to 1...
Embodiment 2
[0039] Embodiment 2, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out the following steps successively:
[0040] 1) Weigh 2kg of silkworm chrysalis protein powder, add 20kg of water and stir well, adjust the pH value to 7.0, heat up to 90°C within 5~10min and keep this temperature for 10min, then cool naturally to 45°C~50°C to keep warm, As a reaction starter; add 40 g of papain (enzyme activity: 800 AU / g) to the reaction starter, continue to incubate and stir continuously for enzymolysis reaction for 3 hours. After the time is up, heat the enzymolysis reaction solution to 100°C within 5-10 minutes, and keep it warm for 10 minutes; then cool it down to room temperature naturally to terminate the reaction;
[0041] Next, centrifuge in a J-6M large-capacity refrigerated centrifuge (BECKMAN company) at 3000r / min at 4°C for 20min to obtain a supernatant of 13.5kg, which is the enzymatic hydrolysis solution; the precipitate obtained by cen...
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Abstract
The invention discloses a method for preparing blood fat reducing peptide by use of silkworm chrysalis, which comprises the following steps: 1) mixing the degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder with water, regulating the pH value to 6.8-7.2, heating to 85-95 DEG C, preserving heat for 8-12 minutes and naturally cooling to 45-50 DEG C; adding papain for an enzymolysis reaction; heating the product of the enzymolysis reaction to 95-105 DEG C and keeping the temperature for 10-15 minutes; naturally cooling to room temperature to terminate the enzymolysis reaction; and centrifuging to obtain enzymolysis liquid; 2) performing decoloration treatment on the enzymolysis liquid at 42-48 DEG C by use of active carbon, and filtering the enzymolysis liquid after the decoloration; and 3) performing ultrafiltration treatment on the rough-filtration enzymolysis liquid under a working pressure of 0.1-0.2MPa and a working temperature of 0-45 DEG C by use of an ultrafiltration membrane to obtain ultrafiltration enzymolysis liquid; and sequentially performing vacuum concentration and freeze drying on the ultrafiltration enzymolysis liquid to obtain the blood fat reducing peptide.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing blood lipid-lowering peptides by using silkworm chrysalis. Background technique [0002] HMG-CoA reductase is the key enzyme that catalyzes the generation of dimethylvaleric acid (MVA) from 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), which is the limit of cholesterol synthesis in vivo. It is also the target of the most important clinical drug for hyperlipidemia at present. HMG-CoA reductase inhibitors are one of the main means of blood lipid-lowering functional ingredients and drug screening. At present, most of the lipid-lowering drugs used in the market are chemically synthesized statins. Although they have a good effect on improving cardiovascular and cerebrovascular diseases caused by hyperlipidemia, long-term use will bring a series of adverse reactions and side effect. [0003] Extracting functional peptides with nutritiona...
Claims
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Application Information
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