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A kind of vacuum low temperature continuous drying method for plant lactic acid bacteria

A plant lactic acid bacteria, vacuum and low temperature technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of cumbersome cleaning, low output, unsuitable for live bacteria, etc., and achieve simple equipment, high survival rate, The effect of strengthening the implementation of industrialization

Active Publication Date: 2020-04-17
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drying of plant lactic acid bacteria adopts vacuum drying, freeze drying, spray drying, etc. The lactic acid bacteria powder produced by freeze vacuum drying technology has the advantages of constant product volume, loose and porous, less water content, convenient transportation and long storage period. Due to the high production cost, the freeze-drying process will also lead to the change and inactivation of the protein structure of some bacteria. Generally, it is mostly used for the preservation of the original species and is not suitable for industrial production. In addition, the output is small and the cleaning is cumbersome. For the same batch of lactic acid bacteria, the equipment needs to be cleaned after each drying; when the hot air spray drying method is used to prepare lactic acid bacteria powder, the required temperature in the spray drying drying tower reaches about 85°C, and the excessive dehydration of the cells will cause the cells to lose their activity. , generally not suitable for the production of live bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A continuous low-temperature vacuum drying method for plant lactic acid bacteria, comprising the steps of:

[0019] (1) Add the drying protectant to the plant lactic acid bacteria solution to prepare a uniform mixture;

[0020] (2) Put the mixture obtained in step (1) into a low-temperature vacuum dryer, the model of the vacuum low-temperature continuous dryer: MJ103-6;

[0021] (3) Preserve the bacteria powder obtained in step (2) in a vacuum.

[0022] The solid content of the mixture prepared in step (1) is 50%-60%.

[0023] The drying protectant described in step (1) is composed of skimmed milk powder, trehalose, soybean protein isolate, maltodextrin, and corn starch, and its mass ratio is (5~6):(5~6):8:(9 ~10): (13~15).

[0024] The low-temperature vacuum drying equipment in step (2) continuously dries for 70 minutes to 90 minutes at a temperature of 45°C~50°C and a vacuum degree of -0.088MPa~-0.099MPa.

Embodiment 2

[0026] A continuous low-temperature vacuum drying method for plant lactic acid bacteria, comprising the steps of:

[0027] (1) Add the drying protectant to the plant lactic acid bacteria solution to prepare a uniform mixture;

[0028] (2) Put the mixture obtained in step (1) into a low-temperature vacuum dryer, the model of the vacuum low-temperature continuous dryer: MJ103-6;

[0029] (3) Preserve the bacteria powder obtained in step (2) in a vacuum.

[0030] The solid content of the mixture prepared in step (1) is 51.25%.

[0031] The drying protectant described in step (1) is composed of skimmed milk powder, trehalose, soybean protein isolate, maltodextrin, and corn starch, and its mass is 22.5 kg, 22.5 kg, 40 kg, 47.5 kg, and 70.0 kg respectively. They are mixed and then added to 1000.0 kg of a solution containing plant lactic acid bacteria to prepare a uniform mixture with a solid content of 50%. The low-temperature vacuum drying equipment continuously dries for 90 min...

Embodiment 3

[0034] The specific implementation is as shown in embodiment 2. Drying protectant skimmed milk powder, trehalose, soy protein isolate, maltodextrin, and corn flour mixture have a mass of 25.3 kg, 25.3 kg, 33.8 kg, 42.3 kg, and 63.3 kg, mix them, and then add them to 1001.1 kg of plant-containing In the solution of lactic acid bacteria, prepare a homogeneous mixture with a solid content of 50%. The low-temperature vacuum drying equipment continuously dries for 90 minutes at a temperature of 45°C and a vacuum degree of -0.099MPa. The number of viable bacteria obtained by detecting the number of colonies is 42.9 billion / g.

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Abstract

The invention discloses a vacuum low-temperature continuous drying method for lactobacillus plantarum. The method comprises the following steps of (1) adding a drying protectant into a lactobacillus plantarum solution to prepare a uniform mixture; (2) feeding the uniform mixture obtained in the step (1) to a vacuum low-temperature continuous drying machine with the model of MJ103-6; and (3) carrying out vacuum storage on bacterial powder obtained in the step (2). The vacuum low-temperature continuous drying method can be used for effectively increasing the survival rate of lactobacillus in the drying process and realizing large-scale production and has relatively strong industrial feasibility.

Description

technical field [0001] The invention belongs to a production method of micro-ecological products, in particular to a continuous low-temperature vacuum drying method for plant lactic acid bacteria. [0002] technical background [0003] With the strengthening of people's health awareness, plant lactic acid bacteria food is more and more favored by consumers. The general preparation process is plant lactic acid bacteria liquid + protective agent + carrier→homogeneous mixing→drying→crushing→product. The drying technology for plant lactic acid bacteria is one of the important steps in the preparation of high-efficiency products. At present, the drying of plant lactic acid bacteria adopts vacuum drying, freeze drying, spray drying, etc. The lactic acid bacteria powder produced by freeze vacuum drying technology has the advantages of constant product volume, loose and porous, less water content, convenient transportation and long storage period. Due to the high production cost, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/25
CPCC12N1/04
Inventor 胡国春郭文江赵福生
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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