Method for improving survival ratio of lactobacillus after freeze drying by liquid nitrogen prefreezing

A lactic acid bacteria and pre-freezing technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of cell membrane and organelle damage, solute damage, and high solute concentration, achieve strong industrialization implementation, and prevent materials from falling. The effect of bubbles, improving the survival rate

CN101503668BInactive Publication Date: 2011-01-05ZHEJIANG UNIV
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2011-01-05
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The invention discloses a method for improving the survival rate of lactobacilli after freeze drying by using prefreezing with liquid nitrogen, which comprises the following steps: (1) collecting well-cultivated bacterial sludge of lactobacilli and adding a protective agent according to a certain mixing ratio; (2) dripping the bacterial sludge into liquid nitrogen for prefreezing; (3) freeze drying the pre-frozen bacterial sludge; and (4) collecting product for storage under vacuum. The method of the invention effectively increases the number of live bacteria after freeze drying by replacing the conventional prefreezing method with prefreezing with the liquid nitrogen, and adopting the certain compound protective agent at the same time, is simple in related materials and convenient operation, and has high industrial implementation performance.
Need to check novelty before this filing date? Find Prior Art

Description

The method of improving the survival rate of lactic acid bacteria after freeze-drying by using liquid nitrogen prefreezing technical field The invention relates to a freeze-drying pre-freezing method, in particular to a pre-freezing method for improving the survival rate of lactic acid bacteria after freeze-drying. Background technique With the improvement of people's quality of life requirements, freeze-dried products are becoming more and more popular. However, due to the fact that the technology of vacuum freeze-drying microorganisms in China is not perfect enough, the number of viable bacteria in freeze-dried products is not high enough, and the freeze-drying technology is still in the development stage. The direct-throwing freeze-dried starter has a high content of viable bacteria, is convenient for storage and transportation, has a high degree of mechanization, and is convenient for industrialized mass production. It is the main development direction of high-efficie...

Examples

Embodiment 1

Embodiment 2

Embodiment 3