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Preparation method of urechis unicinctus visceral protein source zinc chelating peptide

A technology of monocyclic thorn and chelating peptides, which is applied in the field of preparation of animal viscera active polypeptides, can solve the problems of wasting fishery resources, etc., and achieve the effect of easy operation, scientific and reasonable technology

Inactive Publication Date: 2016-11-09
MARINE FISHERIES RES INST OF ZHEJIANG
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Problems solved by technology

People's traditional practice is to eat only the body wall and discard the internal organs, wasting precious fishery resources

Method used

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  • Preparation method of urechis unicinctus visceral protein source zinc chelating peptide
  • Preparation method of urechis unicinctus visceral protein source zinc chelating peptide
  • Preparation method of urechis unicinctus visceral protein source zinc chelating peptide

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Embodiment

[0028] 1) Pretreatment of the viscera of C. monocircleum: chop up the viscera of C. monocircleum and use a tissue blender to homogenize it. The acetone was stirred overnight to degrease, the supernatant was discarded by centrifugation, the precipitate was dried in a blast drying oven at 40°C overnight and then pulverized to obtain the viscera powder of C. monocylicus.

[0029] ) Preparation of the visceral protein of C. unicycle: Take the viscera powder of C. unicycle at a ratio of 1:20 (mass volume ratio, 50 g: 1000 mL), add distilled water, heat in a constant temperature water bath at 80°C for 4 hours, centrifuge and take the supernatant solution, repeat the above steps three times, and finally obtain the supernatant as the experimental sample, which is concentrated and freeze-dried.

[0030] ) Enzymatic hydrolysis of the visceral protein of E. monocylicus: add the sample to distilled water at a ratio of 1:5 (mass volume ratio, 10 g: 50 mL), then adjust the pH to 6.8, add 2%...

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Abstract

The invention discloses a preparation method of an urechis unicinctus visceral protein source zinc chelating peptide. The zinc chelating peptide takes utrechis unicinctus viscera as a raw material, and the product is obtained through viscera pretreatment, protein extraction, enzyme hydrolysis, gel purification and chromatography. The prepared zinc chelating peptide has an amino acid sequence of Phe-Pro-Tyr-Lys-His, and the ESI-MS detection molecular weight is 690.78 Da. The preparation of the zinc chelating peptide provides a technical support for development of zinc supplemental health-care food and drugs, and also provides a new idea for high-valued utilization of the urechis unicinctus viscera.

Description

technical field [0001] The invention relates to a preparation method of an animal visceral active polypeptide, in particular to a zinc chelating peptide derived from the visceral protein of monocyclic echinacea. Background technique [0002] Active biological peptides are favored by more and more consumers due to their wide range of biological activities and their unique effects in medicine, health care products, food and cosmetics, making them a popular choice in the food industry, health care product industry, and daily chemical industry. Competing for the center of attention. Zinc is an essential trace element for the human body, and its deficiency can lead to diseases such as poor growth and development, mental retardation, and decreased immunity in children. Traditional zinc supplements (such as zinc gluconate and zinc citrate) have low absorption and utilization rates, cause severe gastrointestinal irritation, and are not easy to take for a long time. The chelate sal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/20C07K1/16
CPCC12P21/06C07K7/06
Inventor 李佩佩张小军陈思龙举陈荫
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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