Preparation method of urechis unicinctus viscera glycosaminoglycan and application thereof

A single-ringed thorn and glycosaminoglycan technology, applied in the field of extracting glycosaminoglycans, can solve problems such as resource waste, environmental pollution, etc., and achieve the effects of improving purity, simple extraction method and reducing protein content

Inactive Publication Date: 2015-11-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, C. monoringensis has been artificially cultured. Because people only eat its body wall in the traditional way, the viscera of C. monoringensis is discarded as waste in the production and processing link, which causes a great waste of resources and also affects the environment. caused serious pollution

Method used

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  • Preparation method of urechis unicinctus viscera glycosaminoglycan and application thereof
  • Preparation method of urechis unicinctus viscera glycosaminoglycan and application thereof
  • Preparation method of urechis unicinctus viscera glycosaminoglycan and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of Glycosaminoglycans from the Viscera of C. monocylicus

[0023] Take a fresh spinach, insert it through the anus with scissors, cut it open along the abdomen, or cut it along the back, take out all the internal organs, clean the removed internal organs, crush and homogenize them with a meat grinder, and add 4 times the volume After degreasing with acetone, magnetic stirring (200rpm) overnight, suction filtration to extract the precipitate, dry the precipitate and pulverize it to obtain dry powder of the viscera of C. Add distilled water to the prepared dry viscera powder according to the ratio of material to liquid (g / ml) 1:20, extract in a water bath at 80°C for 4 hours, adjust the pH to 7, add papain with 1% weight of dry viscera powder, and digest in a water bath at 60°C 4h, adjust the pH value to 9.0; add 0.5% trypsin by weight of the dry viscera powder, digest in a water bath at 60°C for 3h, adjust the pH value to 7.0, and inactivate at 100...

Embodiment 2

[0024] Example 2: Preparation of Glycosaminoglycan from Visceral Glycosaminoglycan

[0025] Take a fresh spinach, insert it through the anus with scissors, cut it open along the abdomen, or cut it along the back, take out all the internal organs, clean the removed internal organs, crush and homogenize them with a meat grinder, and add 3 times the volume After degreasing with acetone, magnetic stirring (200rpm) overnight, suction filtration to extract the precipitate, dry the precipitate and pulverize it to obtain dry powder of the viscera of C. Add distilled water to the obtained offal dry powder according to the ratio of material to liquid (g / ml) 1:18, and after extracting in a water bath at 75°C for 5 hours, adjust the pH value to 6, add papain with a weight of 1.4% of the dry powder of offal, and digest in a water bath at 65°C 4h, adjust the pH value to 8.5, add 0.6% trypsin by weight of dry viscera powder, digest in a water bath at 65°C for 4h, adjust the pH value to 7.0, ...

Embodiment 3

[0026] Example 3: Preparation of visceral glycosaminoglycans from the monocyclic spiny weeter three

[0027] Take a fresh spinach, insert it through the anus with scissors, cut it open along the abdomen, or cut it along the back, take out all the internal organs, clean the removed internal organs, crush and homogenize them with a meat grinder, and add 4 times the volume After degreasing with acetone, magnetic stirring (200rpm) overnight, suction filtration to extract the precipitate, dry the precipitate and pulverize it to obtain dry powder of the viscera of C. Add distilled water to the obtained offal dry powder according to the ratio of material to liquid (g / ml) 1:18, and after extracting in a water bath at 80°C for 4 hours, adjust the pH value to 6, add papain with a weight of 1.2% of the dry powder of offal, and digest in a water bath at 55°C 5h, adjust the pH value to 8.2, add 0.6% trypsin by weight of dry viscera powder, digest in a water bath at 55°C for 4h, adjust the ...

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Abstract

The invention relates to a preparation method of urechis unicinctus viscera glycosaminoglycan. The method comprises the following steps: using urechis unicinctus viscera, rubbing and homogenizing, degreasing and performing pumping filtration to obtain the urechis unicinctus viscera dry powder, then hydrolyzing the viscera dry powder by papain and trypsin, extracting a viscera glycosaminoglycan crude product from a hydrolysate, and performing dialysis and freeze drying to obtain the viscera glycosaminoglycan. The invention also relates to an application of the viscera glycosaminoglycan in preparation of food, health product and medicine for preventing and adjusting blood fat. The extraction method is simple, yield can reach 6-7%, protein content of viscera glycosaminoglycan can be greatly reduced, purity of glycosaminoglycan is increased, and the method provides a novel approach for preventing and adjusting blood fat.

Description

technical field [0001] The invention relates to a method for extracting glycosaminoglycan from the viscera of Echinococcus monoringensis and the application of the glycosaminoglycan, which belongs to the technical method of comprehensive processing and utilization of Echinococcus monocircleum. Background technique [0002] Urechisunicinctus (Urechisunicinctus), commonly known as sea intestines and sea intestines, belongs to the phylum Echiurioidea Echiurida, the order Xenopneusta, and the family Urchidae. The body surface is covered with granular protrusions of different sizes, the snout is conical, the ventral setae are 1 pair, thick, and there are 9-13 brown tail setae around the anus. It is distributed in Russia, Japan, North Korea and the coast of the Yellow and Bohai Seas in my country. It is the northern part of my country. A common species of benthic organisms in the intertidal subtidal zone and subtidal shallow water zone on coastal muddy banks. C. monoringensis has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L1/09A61P3/06A61P39/06
Inventor 牛庆凤陈荫王斌李涛
Owner ZHEJIANG OCEAN UNIV
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