Prepn. process of and use of thrombus dissolving enzyme

Abstract

This invention discloses a method for manufacturing a fibrinolytic enzyme from whole-body or coelomic fluid of Urechis unicinctus, which comprises the steps of: (1) grinding and / or separating the tissues, (2) centrifuging to obtain the clear supernatant, (3) ultrafiltrating, (4) freeze-drying to obtain freeze-dried powders, or precipitating with acetone at a low temperature and vacuum filtrating to obtain the precipitate, (5) dissolving, purifying through a gel permeation column, and freeze-drying to obtain the fibrinolytic enzyme. The enzyme has good anticoagulation effects, and can be used to manufacture drugs for treating cardiovascular and cerebrovascular diseases.

Description

technical field

[0001] The present invention relates to a preparation method of a thrombolytic enzyme and its application, in particular to a method for preparing a thrombolytic enzyme from the marine organism Echinacea monoringensis, which is applied to the preparation of anticoagulant or anticoagulant for the treatment of cardiovascular and cerebrovascular diseases. Dissolves blood clots. Background technique

[0002] Commonly known as "Sea Intestines", the marine organisms are benthic organisms that live in coastal sediments. They have strong fecundity and abundant resources. The single-ringed weed belongs to the phylum Echiurioidea, the class Echiurida, the order Xenopneusta, and the family Urchidae. At present, the research on E. monoringensis is limited to related basic biology, such as morphological structure, embryonic development, life history, artificial seedling technology and nutritional composition analysis. There has been no report on the development and appl...