The invention discloses a fermentation type seasoning rose cooking wine, which is prepared from the following raw materials: rose, Avicennia marina, olive nutlet, Agrocybe aegerita, lily, Polygonatumodoratum, Polygonatum sibiricum, mulberry, Milkvetch Root, Dendrobium officinale Kimura et Migo, Radix Paeoniae Alba, edible salt, white granulated sugar, Ophiocordyceps Sinensis, tuckahoe, Chinese wolfberry, a microbial fermentation agent, natural spice and aged yellow wine. The invention further discloses a preparation method of the fermentation type seasoning rose cooking wine. According to thepresent invention, the fermentation type seasoning rose cooking wine is rich in nutrition, contains functional ingredients such as polysaccharides and flavone, has unique flavor, can be subjected todirect stirring eating with cooked food without being cooked with food materials, has advantages of long storage time of up to 40 months, strong odor removing and excellent flavor enhancing effect, and further has health effects of digestion benefiting, sleep benefiting, beautifying, face nourishing, mental tranquilization, nerve calming and immune system improving; and the preparation method hasadvantages of cooking wine production process simplifying, high production efficiency, production cost reducing, high product taste and high nutritional value.