Method for preparing phenylethanoid glycosides by use of avicennia marina fruits and application thereof

A technology for the fruit and phenylethanol glycosides of A. chinensis, which is applied in the preparation of sugar derivatives, chemical instruments and methods, and anti-toxic agents. It can solve the problems of large toxic and side effects of organic solvents, complicated extraction and separation processes, and unstable extraction yields. Achieve the effect of stable extraction rate, high application value, anti-oxidation and auxiliary memory improvement

Inactive Publication Date: 2018-11-30
罗永恒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN201610049397.2 discloses a phenylethanol glycoside compound extracted from thistle and its preparation method and use. Thistle was selected as the research object, and modern extraction and separation technology was used to separate and purify a new phenylethanol glycoside from thistle. Ethanol glycoside compound, but the extraction and separation process of this method is complicated, and industrial production is difficult, and petroleum ether, ethyl acetate, n-butanol organic solvents are used in large quantities in this method, and there are disadvantages such as large toxic side effects and large consumption of organic solvents; CN03119204.1 publication A preparation containing phenylethanol glycoside derived from Cistanche tubuloside, its preparation method and application, and a phenylethanol glycoside containing 10-50% echinacoside and 1-15% phylloside Preparation, the method uses water, ethanol, methanol as extraction reagents, reducing the use of other organic toxic reagents, but the extraction yield is unstable

Method used

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  • Method for preparing phenylethanoid glycosides by use of avicennia marina fruits and application thereof
  • Method for preparing phenylethanoid glycosides by use of avicennia marina fruits and application thereof
  • Method for preparing phenylethanoid glycosides by use of avicennia marina fruits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Raw material pre-treatment: 10 kg of fresh A. chinensis fruit is controlled to dry the surface moisture and pulverized with a pulverizer, and passed through a 50-mesh sieve to obtain the B. a.

[0025] (2) Extraction: Add Tween-80 with 0.25% weight of the coarse powder to the coarse powder of the fruit of A. chinensis, add 2 times the volume, that is, 20 L of potassium dihydrogen phosphate and ammonium sulfate aqueous solution with pH 3.8, and ultrasonically treat at 30° C. for 1 hour. After ultrasonic treatment, add ethanol to make the ethanol concentration in the solution 55%, continue ultrasonic extraction for 2 hours, vacuum filter, and collect the extract; extract twice, combine the extract liquid; concentrate under reduced pressure until the volume of the extract is reduced by half, to obtain a concentrate;

[0026] (3) Impurity removal: Add 3.50% chitosan and 5.00% polyvinylpyrrolidone to the concentrated solution, stir evenly at 40°C, let stand for 5h, then c...

Embodiment 2

[0029] (1) raw material pre-treatment: 5 kg of dried A. chinensis fruit is pulverized with a pulverizer, and passed through an 80-mesh sieve to obtain the A. skeletalum fruit coarse powder for subsequent use;

[0030] (2) Extraction: add Tween-20 of 0.3% of the weight of the coarse powder to the coarse powder of the fruit of A. chinensis, add 2 times the volume of potassium dihydrogen phosphate and dipotassium hydrogen phosphate solution of pH3.5 and ultrasonically treat at 30°C 2h, after ultrasonic treatment, add ethanol so that the ethanol concentration in the solution is 75%, continue ultrasonic extraction for 2h, vacuum filtration, and collect the extract; extract 3 times, combine the extract; concentrate the extract under reduced pressure until the volume is reduced by half to obtain a concentrate;

[0031] (3) Impurity removal: add 3.6% chitosan and 2.5% polyvinylpyrrolidone to the concentrated solution, stir evenly at 35°C, let stand for 5h, centrifuge at 25°C, 10000r / mi...

Embodiment 3

[0034] (1) Raw material pre-treatment: 10kg of dried A. chinensis fruit is pulverized with a pulverizer, and passed through an 80-mesh sieve to obtain the A. basilisk fruit coarse powder for subsequent use;

[0035] (2) Extraction: Add 0.5% Tween-80 and 0.2% Tween-40 of the weight of the coarse powder to the coarse powder of the fruit of A. chinensis, add 1 times the volume, that is, 10L of ammonium sulfate aqueous solution with pH 4.5, and perform ultrasonic treatment at 30°C 1h, add ethanol after the ultrasonic treatment to make the ethanol concentration in the solution 65%, continue the ultrasonic extraction for 3h, vacuum filter, collect the extract; extract 4 times, combine the extract; concentrate the extract under reduced pressure until the volume is reduced by half to obtain a concentrate;

[0036](3) Impurity removal: add 4.00% chitosan and 0.25% polyvinylpyrrolidone to the concentrated solution, stir evenly at 40°C, let it stand for 5h, centrifuge at 25°C, 10000r / min ...

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Abstract

The invention discloses a method for preparing phenylethanoid glycosides by the use of avicennia marina fruits and application thereof. The method comprises the following steps: crushing fresh or dryavicennia marina fruits, adding 0.2-0.8% of a surfactant and uniformly mixing, adding 1-3 times volume of an acidic aqueous solution, carrying out ultrasonic extraction, adding an ethanol solution andcontinuously carrying out ultrasonic extraction, extracting for 2-4 times, filtering, mixing the extracts, vacuum-concentrating to a half, and removing impurities by the use of a chitosan/polyvinylpyrrolidone composite flocculant; letting a supernatant pass through a chromatography column filled with reverse phase silica gel, eluting with 20-40% (by volume) of an ethanol solution at the flow velocity of 2-7 mL/min, collecting an ethanol eluate and carrying out vacuum concentration, and freeze-drying. By the method for preparation of a phenylethanoid glycoside extract, content of phenylethanoid glycosides is 70-90%. The extraction efficiency is high, and the process is safe and environmentally-friendly. The method is suitable for large-scale production. The extract obtained by the method has remarkable antioxidant activity and assisted memory improvement effect, and can be used as a main ingredient or one of main ingredients to be applied in relative functional food and medicines.

Description

technical field [0001] The invention belongs to the technical field of separation of natural products, and in particular relates to a method and application of preparing phenylethanol glycosides by using the fruit of A. chinensis. Background technique [0002] Dietera is a true mangrove plant belonging to the Verbenaceae family. It is one of the most important mangrove plant species in my country with the largest distribution area. It is mainly distributed in coastal areas such as Guangdong, Guangxi, Taiwan, Hainan, and Fujian. The fruit of Amaranth is edible, and it is a seasonal food in the subtropical coastal areas of my country. With the increase of research on the fruit of A. chinensis, scholars have found that the fruit of A. chinensis contains a variety of active substances such as alkaloids, polyphenolic compounds and phenylethanol glycosides. [0003] Phenylethyl glycosides are glycoside compounds widely distributed in the plant kingdom. A large number of studies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08A61P39/06A61P25/28A61P25/00
CPCC07H15/18A61P25/00A61P25/28A61P39/06C07H1/08
Inventor 罗永恒
Owner 罗永恒
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