Microwave-assisted extraction process for mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide

A technology of astragalus saponins and astragalus polysaccharides, which is applied in the direction of medical preparations containing active ingredients, plant raw materials, plant/algae/fungus/moss components, etc., to increase the extraction rate, improve the utilization rate of effective substances, and improve the utilization rate of effective substances Effect

Inactive Publication Date: 2014-07-23
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently retrieved literatures are all extracting astragalus saponins or astragalus polysaccharides alone, and there is no literature report on extracting these two types of active ingredients at the same time

Method used

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  • Microwave-assisted extraction process for mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide
  • Microwave-assisted extraction process for mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide
  • Microwave-assisted extraction process for mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Extraction process parameter screening test of the present invention

[0024] 1 Instruments and reagents

[0025] 1.1 Instrument

[0026] HWC-3 microwave extraction experimental equipment (Tianshui Huayuan Medical Equipment Co., Ltd.); UV-6000 ultraviolet-visible spectrophotometer (Shanghai Meipuda Instrument Co., Ltd.); swinging high-speed universal pulverizer (DFY-300, Wenling City Linda Machinery Co., Ltd.); rotary evaporator (RE52CS-1 type, Shanghai Yarong Biochemical Instrument Factory); vacuum drying oven (DZG-6020 type, Shanghai Senxin Experimental Instrument Co., Ltd.); BP61 electronic balance (ten thousandth 1, Germany Satorius); electronic balance (BP211D type, one hundred thousandth, Germany Satorius); UPT-I-10T ultra-pure water device (Chengdu Ultra Pure Technology Co., Ltd.); constant temperature water bath (Jintan City Hongke instrument factory); pipette guns of various models.

[0027] 1.2 Reagent

[0028] Astragalus medicinal material (p...

Embodiment 2

[0083] Example 2 Extraction process of astragalus saponins and astragalus polysaccharides of the present invention

[0084] Precisely weigh 10 grams of astragalus powder (passed through a No. 2 sieve), put it in a microwave extraction device, microwave power 900W, keep the temperature at 70°C, extract 3 times in total, add 158mL of 50% ethanol for the first time, and extract for 10 minutes; the second time Add 140mL of 50% ethanol solution and extract for 10min; add 140mL of distilled water for the third time and extract for 20min. Put the extraction solution in a suction filtration device for suction filtration, transfer the first two extractions to a rotary evaporator, concentrate to a certain volume, add the third extraction, concentrate to about 60mL, add distilled water to a 100mL volumetric flask, and use phenol-sulfuric acid method The content of astragalus polysaccharide in the extract was determined to be 1.720g, the yield was 18.49%, and the extraction rate was 65.32...

Embodiment 3

[0098] Example 3 Extraction process of astragalus saponins and astragalus polysaccharides of the present invention

[0099] Precisely weigh 10 grams of astragalus powder (passed through a No. 2 sieve), put it in a microwave extraction device, microwave power 500W, keep the temperature at 70°C, extract 3 times in total, add 118mL of 50% ethanol for the first time, and extract for 10 minutes; the second time Add 100mL of 50% ethanol solution and extract for 10min; add 100mL distilled water for the third time and extract for 20min. Put the extraction solution in a suction filtration device for suction filtration, transfer the first two extractions to a rotary evaporator, concentrate to a certain volume, add the third extraction, concentrate to about 60mL, add distilled water to a 100mL volumetric flask, and use phenol-sulfuric acid method The content of astragalus polysaccharide in the extract was determined to be 1.772g, the yield was 19.05%, and the extraction rate was 67.30%; ...

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Abstract

The present invention discloses a microwave-assisted extraction process for mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide. The process comprises the following steps: a, weighing a mongolian milkvetch root drug material; b, placing the material in a microwave extraction device, and carrying out extraction three times at a temperature of 70 DEG C, wherein the microwave power is 500-900 W, 50-90% ethanol is added in the first extraction, the first extraction time is 10-30 minutes, 50-90% ethanol is added in the second extraction, the second extraction time is 10-30 minutes, distilled water is added in the third extraction, and the third extraction time is 20-40 minutes; c, concentrating the first extracting solution and the second extracting solution, removing the ethanol from the resulting mixture, then adding the third extracting solution to the ethanol-removed mixture, mixing and concentrating to obtain the extract of mongolian milkvetch root saponin and mongolian milkvetch root polysaccharide. With the extraction process of the present invention, utilization rate of active ingredients in the mongolian milkvetch root drug material is improved, yields of he mongolian milkvetch root saponin and the mongolian milkvetch root polysaccharide in the mongolian milkvetch root drug material are improved, the extraction time is shortened, and the extraction process steps are simplified.

Description

technical field [0001] The invention provides a process for microwave-assisted extraction of astragalus saponin and astragalus polysaccharide. Background technique [0002] Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) is a perennial herb of the leguminous family. After reviewing the relevant literature, until now, the research on the extraction process of Astragalus has mainly focused on the single extraction of astragalus saponins or astragalus polysaccharides, which has caused a great waste of rational utilization of medicinal materials. [0003] Currently retrieved patent literature and non-patent literature have reported that microwaves alone extract astragalus polysaccharides or astragalosides, and the solvents and parameters selected are different, such as Hu Rugui et al. Traditional Chinese Medicine, 2007, 18 (5): 1074-1075), using microwave-assisted extraction technology to extract astragalus saponins from Ast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/481C08B37/00
Inventor 李小芳李航
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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