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Method for extracting flavonoids from Avicennia marina fruit

A technology of flavonoids, white bone soil fruit, applied in the directions of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of limited control of food safety hazards, difficult to achieve environmental protection, leakage of porogen of macroporous resin, etc. Conducive to environmental protection, short operation period, and the effect of reducing the use of organic solvents

Inactive Publication Date: 2019-01-11
罗永恒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above methods mostly use resin as the flavonoid compound in the separation plant, there is a possibility of leakage of the porogen of the macroporous resin, and a large amount of organic reagents are required to elute the target product in the process, which makes it difficult to control the food safety hazard limit and protect the environment. achieve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Pre-treatment of raw materials: Accurately weigh 1.0 kg of dried A. chinensis fruit with a water activity of ≤0.5%, put it into a pulverizer for crushing, and pass through 40 and 50 mesh sieves to obtain A. chinensis fruit powder for later use;

[0030](2) Potassium permanganate wall breaking: add 0.5 times the volume of 500mL potassium permanganate acidic solvent with a concentration of 0.2% to the fruit powder of A. chinensis, stir and mix well, decompress and degas to form a vacuum, and then start the ultrasonic device Ultrasonic treatment was carried out for 5 minutes, centrifugal filtration was carried out, and the filter residue was collected;

[0031] (3) High-shear extraction: put the collected filter residue into a high-shear reaction kettle, then add 3L of ethanol solution with a volume fraction of 50%, react at 10000r / min, 30°C for 10min, centrifuge after the reaction, and collect The extract was reacted twice, the extract was combined, concentrated under...

Embodiment 2

[0036] (1) Pre-treatment of raw materials: Accurately weigh 0.5 kg of dried A. chinensis fruit with a water activity of ≤0.5% and add it to a grinder for crushing. Pass through 80 and 100 mesh sieves to obtain A. chinensis fruit powder for later use;

[0037] (2) Potassium permanganate wall breaking: add 1 times the volume of 500mL potassium permanganate acidic solvent with a concentration of 0.5% to the fruit powder of A. chinensis, stir and mix well, decompress and degas to form a vacuum, and then start the ultrasonic device Ultrasonic treatment was carried out for 5 minutes, centrifugal filtration was carried out, and the filter residue was collected;

[0038] (3) High-shear extraction: put the collected filter residue into a high-shear reactor, then add 5L of ethanol solution with a volume fraction of 60%, react at 20000r / min, 50°C for 5min, centrifuge after the reaction, and collect The extract was reacted twice, the extract was combined, concentrated under reduced pressu...

Embodiment 3

[0043] (1) Pre-treatment of raw materials: Accurately weigh 0.5 kg of dried A. chinensis fruit with a water activity of ≤0.5%, put it into a grinder for crushing, and pass through a 100-mesh sieve to obtain A. chinensis fruit powder for later use;

[0044] (2) Potassium permanganate wall breaking: add 1.5 times the volume of 750mL potassium permanganate acidic solvent with a concentration of 0.3% to the fruit powder of A. chinensis, stir and mix well, decompress and degas to form a vacuum, and then start the ultrasonic device Ultrasonic treatment was carried out for 5 minutes, centrifugal filtration was carried out, and the filter residue was collected;

[0045] (3) High-shear extraction: Add the collected filter residue into a high-shear reactor, then add 2.5L of ethanol solution with a volume fraction of 80%, react at 20000r / min, 30°C for 5min, and centrifuge after the reaction. Collect the extract, react 3 times, combine the extract, concentrate under reduced pressure and v...

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Abstract

The invention relates to a method for extracting flavonoids from Avicennia marina fruit, comprising the following steps: (1) adding potassium permanganate acid solution into dried Avicennia marina fruit powder, filtering and collecting filter residue after vacuum and ultrasonic treatment; 2. extracting flavonoids from Avicennia marina fruit by high shear technology, mixing filter residue and ethanol solution into a high shear reaction kettle, reacting for 5-10min at 10000-20000r/min, reacting for 2-3 times, concentrating and drying that extractive solution to obtain powder; 3) adding the powder a into a carboxyl functionalize ionic liquid/water two-phase to extract flavonoids, heating after the extraction to hydrolyze flavonoids and sugar molecule to obtain flavonoids aglycones; and (4) concentrating and drying to obtain flavonoids extract. The method of the invention is simple and easy to operate, the conditions are easy to control, the extraction time is short, the addition of organic solvent is reduced in the extraction process, the method is more environmentally friendly and safe, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a production technology of flavonoids in mangrove fruit, in particular to a method for extracting flavonoids from A. chinensis fruit. Background technique [0002] Bone soil is a true mangrove plant belonging to the family Verbenaceae Bone soil, which is mainly distributed in coastal areas such as Guangdong, Guangxi, Fujian, Hainan, and Taiwan, with an area of ​​more than 7000hm 2 . The fruit yield of A. chinensis is abundant, and the annual output reaches more than 20,000 tons, but it has not been rationally developed and utilized at present. Studies have shown that the fruit of A. chinensis is rich in flavonoids. Flavonoids are widely found in nature. Studies by scholars at home and abroad have confirmed that flavonoids have various biological activities such as anti-inflammation, anti-oxidation, anti-tumor, and cardiovascular regulation. [0003] At present, there are many patents on the extraction of flavonoids. For exa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/85A61P29/00A61P39/06A61P35/00A61P9/00A61K131/00
CPCA61K36/85A61K2236/15A61K2236/333A61K2236/51A61K2236/53A61P9/00A61P29/00A61P35/00A61P39/06
Inventor 罗永恒
Owner 罗永恒
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