38results about How to "Effective separation and purification" patented technology

The invention provides a bivalent egg yolk antibody against DVH (duck virus hepatitis) as well as a preparation method and an application of bivalent egg yolk antibody. The bivalent egg yolk antibody contains a DHAV (duck hepatitis A virus)-1 type egg yolk antibody against DVH and a DHAV-3 type egg yolk antibody against DVH. The preparation method comprises steps as follows: (1) a DHAV-1 type strain against DVH and a DHAV-3 type strain against DVH are inoculated with an SPF chick embryo and a susceptible duck embryo respectively, an allantoic fluid is obtained, obtained virus fluids are mixed in proportion and inactivated with formalin, and a vaccine is prepared; (2) laying hens are immunized with the vaccine, sampling is performed after immunization for measuring whether the neutralizing titer of DHAV-1 type and DHAV-3 type antigens and antibodies in hyperimmune egg yolk of chickens is larger than or equal to 1:8192, and later, hyperimmune eggs of the chickens are collected; (3) eggshells of the hyperimmune eggs are disinfected, isovolumetric distilled water is added after the egg yolk is collected, and the mixture is stirred and mixed uniformly and then is subjected to pasteurization at the low temperature; purification with an acidified distilled water method and purification with a caprylic acid method are performed; microfiltration and ultrafiltration are performed. The provided bivalent egg yolk antibody is low in cost and high in titer, DVH caused by DHAV-1 and DHAV-3 can be effectively controlled, and remarkable social benefits can be obtained.

The invention discloses a preparation method of anti-lipid peroxide. The preparation method comprises the following steps that a dry monascus fermentation sample is taken, an extraction solvent is added into the sample, extracting is carried out through a lixiviating method so that a rough extraction product can be obtained, then, the rough extraction product is eluted through column chromatography so that purified eluant can be obtained, silica gel thin layer chromatography is conducted on the purified eluant, eluant with the same silica gel thin layer chromatography results is combined, column chromatography elution is carried out again, and then the anti-lipid peroxide is obtained. Monascus serves as a raw material to prepare the anti-lipid peroxide for the first time, the rough extraction product of the monascus sample is extracted firstly, optimal column chromatography eluant is screened out according to the activity distribution situation and the solubility in different solvents, and the anti-lipid peroxide is effectively separated and purified. The preparation method is simple and easy to realize, the consumed time is short, the raw material is easy to get and low in cost, and batch production of the anti-lipid peroxide is achieved.

The invention relates to a crystal form of a key intermediate compound I of a tyrosine kinase inhibitor lapatinib (a lapatinib imine crystal form for short) and a lapatinib imine p-toluenesulfonate crystal form (a crystal form of a compound II for short), and a preparation method for the crystal forms. The crystal shape of a lapatinib imine crystal is determined through powder X-ray diffraction detection; and the crystal shape of a lapatinib imine p-toluenesulfonate crystal is determined through powder X-ray diffraction detection. The two crystal forms have the advantages that: lapatinib imine can be separated from reaction liquid and purified, the crystal forms have positive significance for improving the quality of lapatinib, and the preparation process is simple and suitable for industrial production.

This invention relates to extraction process of camel colostrum immunoglobulin IgA and IgG, belongs to biochemistic product field. The camel colostrum is the most prolific natural source of immunoglobulin, and is one of the biotic resources that has most concentrated immune factor. This invention supplies a craft of using gradient elution to extract IgA and using ultrafiltration and metal chelatechromatography to extract IgG. By test has show that in product IgA basicly has no other impurity, purity of IgG can reach 97 percent and above.

A method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in a caramel pigment comprises the following steps: 1, preparing a solution of a sample to be detected; 2, detecting the sample; and 3, analyzing result. The method has the advantages of rapid detection, high sensitivity, high accuracy and the like.

A filter-type gas separating-cleaning apparatus with quickly moving filter layer features that its filter cylinder rotating at high speed is composed of external filter net, supporting posts, internal filter net, shaft sleeve, shaft disk and supporting rings, and the gas containing solid particles and / or liquid drops is filtered by said filter cylinder. Its advantage is use of big-mesh filter material to remove fine solid particles and / or liquid drops, resulting in high filter speed, and easy cleaning.

The invention discloses a preparation method of a granisetron intermediate. The preparation method comprises the following steps: step 1, carrying out a Mannich reaction on acetone dicarboxylic acid represented by a formula III to obtain pseudopelletierine represented by a formula IV; step 2, carrying out a reaction on the pseudopelletierine and hydroxylamine to prepare 3-pseudopelletierine oximerepresented by a formula V; and step 3, carrying out preparation by adopting one of the following schemes: (1) carrying out catalytic reduction on the 3-pseudopelletierine oxime through sodium bis(2-methoxyethoxy)aluminumhydride and Lewis acid to obtain a crude product of endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane represented by a formula I, and directly using the crude product of endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane to prepare granisetron, or purifying the crude product of endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane for preparing granisetron; and (2) carrying out catalytichydrogenation reduction on the 3-pseudopelletierine oxime through Raney nickel to obtain a mixture of endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane, purifying the mixture to obtain endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane represented by the formula I, and using the endo-3-amine-9-methyl-9-azabicyclo[3,3,1]nonane used for preparing granisetron. The method has the advantages of mild reaction conditions, high reaction yield and low cost, and is suitable for industrial production.

The invention provides a purification method for whey protein. The purification method comprises: taking mammal milk as a raw material, performing pretreatment to obtain whey, and performing hydrophobic chromatographic separation, so as to obtain purified alpha-whey alhumin and beta-lactoglobulin. The invention also provides a method for obtaining purified recombinant human alpha-whey alhumin and animal endogenous alpha-whey alhumin and beta-lactoglobulin. The method comprises: when whey protein contains recombinant human alpha-whey alhumin, firstly performing anion exchange chromatography before hydrophobic chromatographic separation, so as to separate recombinant human alpha-whey alhumin from endogenous alpha-whey alhumin and beta-lactoglobulin, and then performing hydrophobic chromatographic separation, so as to obtain purified recombinant human alpha-whey alhumin and animal endogenous alpha-whey alhumin and beta-lactoglobulin.

The invention discloses an active ingredient of Tibetan salvia tanshinone and its extraction method and application. The extraction method comprises the following steps: drying and crushing Tibetan salvia miltiorrhiza, then soaking in water, and adding 6-8 times the amount of 60-60 90% ethanol, ultrasonic extraction for 60-90min, extraction 1-2 times; Concentrate the ultrasonic extract, then extract with ethyl acetate, collect the extract, concentrate under reduced pressure to obtain a fat-soluble extract, and then extract the extract Dissolved to the corresponding concentration, separated and purified by medium-pressure preparative chromatography. The method of the invention is simple to operate, can effectively separate and purify target components, greatly improves its purity and extraction efficiency, and can fully exert its medicinal value.

The invention discloses a separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid. The method utilizes high-speed counter-current chromatography to separate and purify the Arctic marine rhodococcus B7740 produced isoprenoid. The method has the advantages that the isoprenoid produced by the Arctic marine rhodococcus B7740 is effectively separated and purified by the high-speed counter-current chromatography, so as to obtain three types of rare marine-derived carotenoids; the three types of rare marine-derived carotenoids are further identified by high-precision mass spectrometry.