38results about How to "Effective separation and purification" patented technology

A filter-type gas separating-cleaning apparatus with quickly moving filter layer features that its filter cylinder rotating at high speed is composed of external filter net, supporting posts, internal filter net, shaft sleeve, shaft disk and supporting rings, and the gas containing solid particles and/or liquid drops is filtered by said filter cylinder. Its advantage is use of big-mesh filter material to remove fine solid particles and/or liquid drops, resulting in high filter speed, and easy cleaning.

The invention discloses a production method of ergosterol. The production method includes the following steps: adding an extracting solvent to a dried Monascus purpureus-fermented sample, extracting by an extraction process to obtain crude extract, eluting the crude extract by column chromatography to obtain primary purified eluent, subjecting the primary purified elution to silica gel thin-layer chromatography, combining eluents with same silica gel thin-layer chromatography results and performing column chromatography again to obtain secondary purified eluent, removing the solvent from the secondary purified eluent, and recrystallizing through petroleum ether to obtain ergosterol. The preparation of ergosterol from Monascus purpureus as material is provided for the first time, crude extract of the sample is extracted first, and an optimal column chromatography eluent is screened then according to distribution conditions and dissolutions in different solvents, so that ergosterol is isolated and purified effectively. The production method of the ergosterol is simple, easy to implement and low in time consumption, the material is easy to obtain and low in cost, and batch production of ergosterol is achieved.

The invention discloses a method for preparing flaxseed gum oligosaccharides by a hydrogen peroxide oxidation degradation technology. The flaxseed gum oligosaccharides (FGOSs) are prepared from commercial flaxseed gum by adopting a high pressure assisted hydrogen peroxide oxidation degradation technology through dynamic dissolving, oxidative degradation, suction filtering, decolorizing, ultrafiltration, dialysis, concentration and freeze-drying. The FGOSs obtained in the invention are a semisolid viscous substance, have a light reddish brown color and special fragrance, can be easily dissolved in water, are insoluble in ethanol and other organic solvents, can easily absorb moisture, are a typical carbohydrate substance, and contain uronic acid; the FGOSs have uniform components, the average molecular weight is 1047 Da, the FGOS have a pyranose ring structure, belong to pyranose, and are composed of the following seven monosaccharides: rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose, and the FGOS have certain anti-oxidation ability, and have a high removal rate on hydroxyl free radicals, DPPH free radicals, ABTS free radicals and superoxide anion free radicals.

The invention discloses a detecting method for byproducts 2-methylimidazole, 4-methylimidazole and 2-acetyl-4- tetrahydroxyl-buthylimidazole in caramel pigment by combining solid-phase extraction with high-efficiency liquid chromatography. The method comprises the following steps: 1. preparing a to-be-detected sample solution; 2. detecting a sample; and 3. analyzing results. The method disclosed by the invention has the characteristics of quick detection, high sensitivity, high degree of accuracy, low cost, strong universality, and the like.

The invention discloses a phenolic aldehyde-based super-hydrophilic carbon nanofiber net film and a preparation method thereof, wherein the preparation method comprises the steps: firstly, adding a mixture of thermoplastic phenolic resin and thermosetting phenolic resin, a spinning polymer and hydrophilic nanoparticles or a precursor of the hydrophilic nanoparticles into a solvent, stirring and dissolving to obtain a spinning solution, then carrying out electrostatic spinning, and preparing a composite precursor nano-fiber net film; performing heat treatment on the composite precursor nano-fiber net film, and performing multi-temperature-section carbonization treatment under the protection of high-purity nitrogen to prepare a primary carbon nano-fiber net film; and finally performing surface hydrophilicity enhancement treatment on the primary carbon nano-fiber net film to obtain the super-hydrophilic carbon nano-fiber net film. According to the method disclosed by the invention, a mixture of thermoplastic phenolic resin and thermosetting phenolic resin is used as a main carbon source, the hydrophilic nanoparticles are introduced, and after surface hydrophilicity enhancement treatment, the carbon nanofiber net film with a super-hydrophilic characteristic is obtained and can be used for efficient separation and purification of an oil-in-water type oil-water mixture.

The invention relates to the technical field of preparative liquid chromatography separation, and particularly discloses a two-dimensional high-pressure preparative liquid chromatography system and a separation and purification method for low-content target components in a medicine. The system comprises a first-dimensional high-pressure preparative liquid chromatography system, a two-position six-way switching valve, a multi-position selection valve, a trapping column and a second-dimensional high-pressure preparative liquid chromatography system, the second-dimensional high-pressure preparative liquid chromatography system comprises a second mixer and a two-dimensional separation column; the first-dimensional high-pressure preparative liquid chromatography system is sequentially communicated with the two-position six-way switching valve, the multi-position selection valve and the trapping column, and the outlet end of the trapping column is communicated with the two-position six-way switching valve again; and the second mixer is sequentially communicated with the two-position six-way switching valve and the two-dimensional separation column. The method comprises the following steps: detecting a sample by using a one-dimensional system, and trapping a target component to a trapping column; and switching the two-position six-way switching valve, back-washing out captured components, and carrying out two-dimensional detection. The method disclosed by the invention has the advantage of efficiently separating and purifying impurity components with extremely low content in the medicine.

The invention discloses a method for removing heavy metal ions in wastewater through ion exchange resin and a resin regeneration method and belongs to the technical field of environmental protection.The method comprises the following steps that 1, the concentration of Cl- in wastewater with heavy metal ions Mn+ is adjusted, so that the heavy metal ions are transformed into Mn+-Cl type stable complexation negative ions; 2, negative ion exchange resin is adopted for adsorbing the Mn+-Cl type complexation negative ions in the transformed wastewater; 3, a FeCl4- solution is adopted for desorbingthe negative ion exchange resin adsorbing the Mn+-Cl complexation negative ions; 4, the pH value of a desorption solution generated in the third step is adjusted until a metal hydroxide precipitate isformed through Mn+, and through solid-liquid separation, solids are taken as dangerous solid waste for treatment; ferric chloride is added to a supernate, the pH is adjusted, and the FeCl4- solutionis prepared for application in the third step. According to the method, the aim is achieved successfully that heavy metal ions in the wastewater with interference ions are removed with low cost underthe industrial scale. Regeneration is complete, and the service life of the resin is effectively prolonged.

The invention relates to a method for determining verbascoside in blood nourishing and brain-refreshing water extract. The method comprises the following steps: 1) reference substance preparation method: weighing a verbascoside reference substance, and adding methanol to prepare a standard reference solution; 2) a preparation method of a test solution: weighing the blood nourishing and brain refreshing water extract, adding methanol to dissolve, loading on a solid phase extraction column, eluting with a methanol solution, collecting eluent, and fixing the volume with methanol; and 3) a determination method: respectively taking the reference substance solution and the test solution, injecting into an ultra-high performance liquid chromatograph, recording a chromatogram, calculating the verbascoside content of the test solution by using an external standard method according to the peak area, and converting to obtain the verbascoside content in the blood nourishing and brain refreshing water extract.

The invention provides a method for industrially purifying sheep placenta polypeptides. The method comprises the following steps: preprocessing and homogenating sheep placentas, continuously carrying out three-time refrigeration under a vacuum condition at different temperatures, filtering, refrigerating, drying, and crushing to prepare lyophilized sheep placenta powder; carrying out a supersonic wave and microwave heating compounding process of the lyophilized sheep placenta powder for dispersion to form a tissue homogenate; cooling the tissue homogenate, centrifuging, and taking the obtained supernatant; adding a mixed organic solvent into the supernatant, fully reacting, centrifuging, taking the obtained new supernatant, adding acetone to the new supernatant for extraction, and taking the obtained precipitate; and dissolving the precipitate, letting the obtained precipitate solution in an ultrafiltration membrane assembly separator and a gel column combined separator for combined purification, and carrying out concentration lyophilizing to obtain purified sheep placenta polypeptides. The method has a low production cost and is suitable for the industrialized production, and a detection result shows that the purity of the sheep placenta polypeptides prepared through the method reaches above 90%.

The invention belongs to the technical field of plant extraction and discloses a method for extracting lactoflavin from phyllanthus emblica. According to the method for extracting the lactoflavin in the phyllanthus emblica, three methods of phyllanthus emblica extraction, normal phase silica gel column chromatography and a preparation liquid chromatograph are cooperatively used, so that the methodis a novel and effective separation and purification method, the anti-inflammatory active ingredient lactoflavin in the phyllanthus emblica is effectively separated and purified, separation steps arefew, the purity of the separated lactoflavin is high, and the separation purity of the lactoflavin can reach 95% or above. The lactoflavin is separated from phyllanthus emblica for the first time, and reference is provided for separation and purification of active ingredients in extracts of other natural products.

The invention provides a simple and efficient method for two-liquid phase extraction, separation and purification, and preparation of high-purity tea polyphenols, which at least includes the steps of: preparing tea raw materials; tea raw materials, water phase, and organic phase in a mass-volume ratio of 1g:5~ 20mL: Mix 5-20mL; mix the tea raw materials with the water phase evenly, and keep the water phase and the organic phase separated; separate the organic phase after extraction, and use it as the crude extract; mix the crude extract with the water phase and the adsorbent according to the mass volume Mix 10mL: 10mL: 0.5~2g; mix the adsorbent with the water phase evenly, and keep the water phase and the organic phase separated; separate the organic phase again to obtain a refined extract. The invention selectively extracts tea polyphenols through a two-liquid phase system, and combines with a pretreatment adsorbent to reduce the extraction of impurities and improve the purity of finished products. At the same time, no toxic and harmful substances are introduced, which increases the safety of finished products. The reagent used in the invention is easy to obtain, can be recycled and re-entered into the process, has high utilization rate, and effectively reduces cost.

The invention discloses a preparation method of anti-lipid peroxide. The preparation method comprises the following steps that a dry monascus fermentation sample is taken, an extraction solvent is added into the sample, extracting is carried out through a lixiviating method so that a rough extraction product can be obtained, then, the rough extraction product is eluted through column chromatography so that purified eluant can be obtained, silica gel thin layer chromatography is conducted on the purified eluant, eluant with the same silica gel thin layer chromatography results is combined, column chromatography elution is carried out again, and then the anti-lipid peroxide is obtained. Monascus serves as a raw material to prepare the anti-lipid peroxide for the first time, the rough extraction product of the monascus sample is extracted firstly, optimal column chromatography eluant is screened out according to the activity distribution situation and the solubility in different solvents, and the anti-lipid peroxide is effectively separated and purified. The preparation method is simple and easy to realize, the consumed time is short, the raw material is easy to get and low in cost, and batch production of the anti-lipid peroxide is achieved.