2results about How to "Effective separation and purification" patented technology

The application provides a pertussis antigen recombinant expression vector and engineering bacteria and application thereof. The pertussis antigen recombinant expression vector comprises a resistance screening gene, an upstream recombinant nucleic acid fragment of a pertussis exotoxin gene and a downstream recombinant nucleic acid fragment, wherein the upstream and downstream recombinant nucleic acid fragments can respectively perform homologous recombination with the upstream and downstream of the pertussis exotoxin gene. The application takes the pertussis bacillus as a host, constructs an engineering bacteria ΔPT strain for producing FHA and PRN by knocking out the PT gene, simplifies the production process of the FHA and PRN antigens, maintains the high purity and immunogenicity of the FHA and PRN antigens, eliminates the interference of the PT protein in the production of the FHA and PRN antigens, avoids the PT protein detoxification process, and eliminates the risk of possible PT protein toxicity reversal.

This utility model relates to a treatment device for methanol waste liquid from benzalkonium chloride, belonging to the field of benzalkonium chloride technology. The treatment device includes: a tubular reactor; a discharge pipe at one end of the tubular reactor is connected to a rising film evaporator; a discharge pipe at the top of the rising film evaporator is connected to a gas-liquid separator; and a discharge pipe at the top of the gas-liquid separator is connected to a methanol distillation column. A recovery mechanism is provided on one side of the methanol distillation column. Through the tubular reactor, rising film evaporator, and methanol distillation column, the methanol waste liquid is reacted and distilled to obtain qualified methanol product, turning the methanol waste liquid into a by-product, saving treatment costs. During methanol treatment, sodium acetate impurities can be effectively separated and reused, allowing for the recycling of methanol waste liquid. The device can effectively separate and purify methanol waste liquid from benzalkonium chloride, improving the efficiency of the treatment.