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Method for separating and purifying blood-glucose-reducing longan aril polysaccharides

A longan meat polysaccharide, separation and purification technology is applied in the field of separation and purification of hypoglycemic longan meat polysaccharide, which can solve the problems of polysaccharide molecular weight dispersion, single extraction solvent, and inability to conduct targeted research, and achieve the effect of reducing blood sugar.

Active Publication Date: 2018-05-04
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on polysaccharide extraction technology at home and abroad mostly adopts the traditional hot water extraction method, the extraction solvent is single, and the polysaccharides are difficult to release from the plant cells; in addition, the hot water extraction method requires a long extraction time and high extraction temperature. It is easy to cause the hydrolysis of polysaccharides, thereby reducing the biological activity of polysaccharides; moreover, the molecular weight of polysaccharides prepared by the current process is relatively dispersed and chaotic, and it is impossible to carry out targeted research

Method used

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  • Method for separating and purifying blood-glucose-reducing longan aril polysaccharides
  • Method for separating and purifying blood-glucose-reducing longan aril polysaccharides
  • Method for separating and purifying blood-glucose-reducing longan aril polysaccharides

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Embodiment 1

[0021] A method for separating and purifying hypoglycemic longan meat polysaccharide, comprising the following steps:

[0022] (1) Stir and pulverize: collect fresh longan meat, put it into a blender, stir at a speed of 3000r / min for 15min, obtain longan meat slurry, and set aside;

[0023] (2) Preparation of the total extract of longan meat: at 70°C, extract the longan meat slurry with a 70% ethanol solution for 3 hours, using a solid-liquid ratio of 1:20, collect the extract, and reduce it at 50°C. Concentrate under pressure to obtain the total extract of longan meat;

[0024] (3) Extraction: including petroleum ether extraction, ethyl acetate extraction and n-butanol extraction;

[0025] Petroleum ether extraction: Transfer 200mL of the total extract of longan meat into a 1000mL separatory funnel, add 400mL of petroleum ether, shake, and let stand. After the solution is completely separated, the upper layer solution is the petroleum ether extract;

[0026] Ethyl acetate e...

Embodiment 2

[0031] A method for separating and purifying hypoglycemic longan meat polysaccharide, comprising the following steps:

[0032] (1) stirring and crushing: collect fresh longan meat, put it into a blender, stir at a speed of 3500r / min for 18min, obtain longan meat slurry, and set aside;

[0033] (2) Preparation of the total extract of longan meat: at 71°C, extract the longan meat slurry with a 70% ethanol solution for 3.5 hours, the ratio of solid to liquid used is 1:20, collect the extract, and carry out at 50°C Concentrate under reduced pressure to obtain the total extract of longan meat;

[0034] (3) Extraction: including petroleum ether extraction, ethyl acetate extraction and n-butanol extraction;

[0035] Petroleum ether extraction: Transfer 200mL of the total extract of longan meat into a 1000mL separatory funnel, add 400mL of petroleum ether, shake, and let stand. After the solution is completely separated, the upper layer solution is the petroleum ether extract;

[00...

Embodiment 3

[0041] A method for separating and purifying hypoglycemic longan meat polysaccharide, comprising the following steps:

[0042] (1) Stir and pulverize: collect fresh longan meat, put it into a blender, stir at a speed of 4000r / min for 16min, obtain longan meat slurry, and set aside;

[0043] (2) Preparation of the total extract of longan meat: at 72°C, extract the longan meat slurry with 70% ethanol solution for 4.5 hours, the ratio of solid to liquid used is 1:20, collect the extract, and carry out at 50°C Concentrate under reduced pressure to obtain the total extract of longan meat;

[0044] (3) Extraction: including petroleum ether extraction, ethyl acetate extraction and n-butanol extraction;

[0045] Petroleum ether extraction: Transfer 200mL of the total extract of longan meat into a 1000mL separatory funnel, add 400mL of petroleum ether, shake, and let stand. After the solution is completely separated, the upper layer solution is the petroleum ether extract;

[0046] E...

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Abstract

The invention discloses a method for separating and purifying blood-glucose-reducing longan aril polysaccharides. The method comprises the following steps: (1) stirring and grinding; (2) preparing total extract of longan ari; (3) extracting; and (4) separating and purifying longan aril polysaccharides, namely enabling 500mL of longan aril crude polysaccharide solution to pass through filter membranes with molecular mass Mr of 30kD, 10kD, 3kD and 650D by adopting an ultrafiltration system, thereby respectively obtaining five longan aril polysaccharide solutions of which the Mr is more than 30kD, the Mr is more than 10kD and less than 30kD, the Mr is more than 3kD and less than 10kD, the Mr is more than 650D and less than 3kD and the Mr is less than 650D, wherein the polysaccharide with theMr of being more than 10kD and less than 30kD is the blood-glucose-reducing longan aril polysaccharide. According to the method disclosed by the invention, the blood-glucose-reducing longan aril polysaccharides can be efficiently separated and purified, the longan aril polysaccharides with different molecular weights can be prepared, and a research basis is provided for expanding further development of the longan aril polysaccharides.

Description

【Technical field】 [0001] The invention relates to the field of plant extraction, in particular to a separation and purification method for hypoglycemic longan meat polysaccharide. 【Background technique】 [0002] Longan meat is extremely rich in vitamin C and potassium, and also contains a lot of magnesium and copper. It has the effect of nourishing qi and blood, and has a significant effect on treating fatigue, insomnia, and forgetfulness. Longan meat is rich in nutrition, and the main component is water, chloroform, and ethanol extracts of sugar longan meat, all of which have significant hypoglycemic and antioxidant activities. [0003] Polysaccharides are sugar chains joined by monosaccharides through glycosidic bonds, and are widely distributed in plants, animals and microorganisms. Polysaccharides are not a pure chemical substance, but a mixture of substances with different degrees of polymerization. In recent years, people have realized that polysaccharides are not on...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/10
CPCC08B37/00C08B37/0003
Inventor 何雪梅孙健李丽李志春刘国明郑凤锦辛明盛金凤唐雅园李昌宝李杰民零东宁周主贵
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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