Starlike magnetic response organic adsorption material and preparation method thereof

An organic adsorbent, magnetic response technology, applied in chemical instruments and methods, inorganic chemistry, other chemical processes, etc., can solve the problem of lack of monodisperse polymers, low specific surface area and active sites, and adsorption efficiency of adsorbent materials. Low problems, to achieve the effects of excellent affinity, efficient and rapid separation and purification, and increased diversity

Inactive Publication Date: 2018-01-09
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this kind of polymer microspheres has a big disadvantage in practical application: it is not easy to separate
However, during the specific experiment process, the researchers found that with the continuous progress of the polymerization reaction, the particle size distribution of the obtained polymer was very wide, and no monodisperse polymer could be obtained. At the same time, due to the large particle size prepared, this type of polymerization The lower specific surface area and active sites of the adsorbent material will lead to lower adsorption efficiency of the final adsorbent material.

Method used

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  • Starlike magnetic response organic adsorption material and preparation method thereof
  • Starlike magnetic response organic adsorption material and preparation method thereof
  • Starlike magnetic response organic adsorption material and preparation method thereof

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Experimental program
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Embodiment 1

[0038] Composition of raw materials measured in parts by mass:

[0039] dispersed phase

[0040]

[0041]

[0042] Continuous phase:

[0043] 70 parts of ethanol

[0044] 10 parts of toluene

[0045] 0.8 parts of polyvinylpyrrolidone

[0046] The polyvinylpyrrolidone described in the present invention is PVP K3000 type.

[0047] The oleic acid modified ferric oxide described in the present invention is a 5% cyclohexane solution.

[0048] A method for preparing a magnetically responsive star-shaped organic adsorbent material for separating and purifying proteins, specifically comprising the following steps:

[0049] Quantitative polyvinylpyrrolidone and ethanol were weighed into the reactor, stirred and dissolved at room temperature at a speed of 200 rpm, and then an appropriate amount of toluene was added to prepare a continuous phase. Add glycidyl methacrylate, dodecyl alcohol, cyclohexanol, and oleic acid-modified ferric iron tetroxide to another container, mix e...

Embodiment 2

[0051] Composition of raw materials measured in parts by mass:

[0052] Dispersed phase:

[0053]

[0054] Continuous phase:

[0055] Ethanol 90 parts

[0056] 15 parts of toluene

[0057] 1 part polyvinylpyrrolidone

[0058] The polyvinylpyrrolidone described in the present invention is PVP K3000 type.

[0059] The oleic acid modified ferric oxide described in the present invention is a 5% cyclohexane solution.

[0060] A method for preparing a magnetically responsive star-shaped organic adsorbent material for separating and purifying proteins, specifically comprising the following steps:

[0061] Quantitative polyvinylpyrrolidone and ethanol were weighed into the reactor, stirred and dissolved at room temperature at a speed of 200 rpm, and then an appropriate amount of toluene was added to prepare a continuous phase. Add glycidyl methacrylate, dodecyl alcohol, cyclohexanol, and oleic acid-modified ferric iron tetroxide to another container, mix evenly and place in a...

Embodiment 3

[0063] Composition of raw materials measured in parts by mass:

[0064] Dispersed phase:

[0065]

[0066] Continuous phase:

[0067] 60 parts of ethanol

[0068] 10 parts of toluene

[0069] 0.5 parts of polyvinylpyrrolidone

[0070] A method for preparing a magnetically responsive star-shaped organic adsorbent material for separating and purifying proteins, specifically comprising the following steps: weighing quantitative polyvinylpyrrolidone and ethanol into a reactor, stirring and dissolving at room temperature at a speed of 200 rpm A suitable amount of toluene was added to obtain a continuous phase. Add glycidyl methacrylate, dodecanol, cyclohexanol, and oleic acid-modified ferric iron tetroxide to another container, mix evenly and place in an ultrasonic cleaner for ultrasonic dispersion for 10 minutes to obtain a dispersed phase. Add the dispersion to the continuous phase, and add a certain amount of azobisisobutyronitrile dissolved in toluene at the same time, ...

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Abstract

The invention relates to a starlike-structured adsorption material and a preparation method thereof and belongs to the technical field of a preparation technology of a high polymer material. The starlike magnetic response material used for efficiently, rapidly and conveniently separating and purifying protein comprises raw materials as follows: a continuous phase and a dispersion phase, wherein the continuous phase comprises ethanol, toluene and polyvinylpyrrolidone, and the dispersion phase comprises GMA (glycidyl methacrylate), EDMA (ethylene dimethacrylate ), azodiisobutyronitrile, dodecanol, cyclohexanol and Fe3O4. Polymeric microspheres are controlled to perform surface crosslinking self-assembly with a dispersion polymerization method, so that a starlike magnetic response polymer adsorbent is formed. The prepared starlike magnetic response organic adsorbent not only has high specific surface area, high adsorption sites and high adsorbing capacity of a microsphere adsorbent, but also has the characteristics of large macro particle size, high magnetic response strength and the like, two characteristics including efficient adsorption and convenient separation of a biological macromolecular adsorbent are integrated, and a separation material with good performance is provided for the field of the bioseparation materials. The adsorption capacity of the material for BSA (bovineserum albumin) can reach 108 mg per g of a dry-based material.

Description

technical field [0001] The invention relates to a star-shaped adsorption material and a preparation method thereof, more specifically to a polyester adsorption material for efficient separation and purification of proteins and a preparation method thereof, belonging to the technical field of polymer material preparation technology. Background technique [0002] With the continuous development of biochemical industry, biological macromolecules are continuously used in various fields, making bioseparation technology a research hotspot in the biochemical industry. Due to their large specific surface area, ease of modification, and high mechanical strength, polymer microspheres have been widely used in many applications in bioseparation, including enzyme immobilization, cell separation, immunoassays, protein purification, and Used as a drug site-specific carrier. However, this kind of polymer microspheres has a big disadvantage in practical application: it is not easy to separa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/28B01J20/26B01J20/30
Inventor 杜开峰但顺民
Owner SICHUAN UNIV
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