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Phosphorus-dissolving penicillium oxalicum with broad spectrum acid production feature

A technology of Penicillium oxalicum and strains, applied in the field of environmental biology, can solve limited problems, achieve the effect of improving soil fertility, good application value, and promoting comprehensive treatment of heavy metal soil pollution

Active Publication Date: 2019-08-16
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few phosphorus-solubilizing fungi with broad-spectrum organic acid metabolism characteristics at home and abroad. Therefore, the research on the application of phosphorus-solubilizing bacteria to soil remediation is still very limited.

Method used

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  • Phosphorus-dissolving penicillium oxalicum with broad spectrum acid production feature
  • Phosphorus-dissolving penicillium oxalicum with broad spectrum acid production feature
  • Phosphorus-dissolving penicillium oxalicum with broad spectrum acid production feature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] [Implementation 1] Screening of Phosphorus Solubilizing Fungi

[0027] The experimental soil samples were collected from the activated sludge of the sewage treatment plant in Yunxi District Industrial Park, Yueyang City, Hunan Province, and were air-dried for 48 hours immediately after collection. After removing excess impurities, they were ground through a 20-mesh soil sieve, and the excess soil samples were stored in a refrigerator at 4°C. . Take 10g of sieved soil sample, add 100mL sterilized liquid enrichment medium (containing sterile glass beads) (enrichment medium components: peptone 10g / L, NaCl 1.0g / L, KH 2 PO 3 1.0g / L, pH=7), shaking at 150rpm for 7 days. After culturing for 1 week, take 1 mL of the culture solution in the enriched medium, and take 1 mL of the supernatant and dilute it to 10 -5 , take 10 of them -4 ,10 -5 ,10 -6 200 μL of the solutions under the three dilution gradients were placed in the phosphate-dissolving medium with calcium phospha...

Embodiment 2

[0029] [Example 2] identification of bacterial strains

[0030] Inoculate the purified bacterial strain on solid PDA medium (ingredients: potato 200g / L, glucose 20g / L, agar 20g / L, pH=7) and cultivate for 5 days to observe the bacterial colony morphological characteristics of the bacterial strain on the solid plate, and then pick some Observe the microstructure of the bacterial colony under an optical microscope, and record the morphological characteristics of the bacterial strain: the colony is fluffy, the interbranches are bifurcated, mostly trident, and the center is asymmetrically bifurcated; the color of the spore is green, and the shape is oval , spherical, about 0.10 μm in diameter; spore peduncle short and thick, with obvious branches, fork-like acute-angled branches, asymmetrical branches, about 0.25 μm in length.

[0031] Fungal ITS rDNA sequencing included strain DNA extraction, ITS rDNA in vitro amplification (using ITS universal primers ITS1 and ITS4) and gene sequ...

Embodiment 3

[0032] [Example 3] Degradation characteristics analysis of bacterial strains to calcium phosphate and iron phosphate

[0033] Using liquid phosphate-dissolving medium, the final concentrations after adding calcium phosphate and iron phosphate were set to 5g / L and 2g / L respectively. Before the measurement, inoculate the strain in the phosphorus-dissolving slant medium and cultivate it, activate it at 28°C for 72 hours, then take 1mL of sterile water, add it to the slant, and scrape the spores and hyphae of the slant fungus into the sterile water to form a spore suspension solution was added to the culture medium at a volume ratio of 5%. All the cultures were shaken at 28° C. for 14 days based on a shaker at 150 rpm, and the pH value and soluble phosphorus content of the medium were measured respectively. Finally, PSF-4 can dissolve 2071mg / L of calcium phosphate with a phosphorus dissolution rate of 100% for 5g / L calcium phosphate, and 198mg / L for 2g / L iron phosphate with a pho...

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Abstract

The invention discloses phosphorus-dissolving penicillium oxalicum with a broad spectrum acid production feature. The preservation number of the phosphorus-dissolving penicillium oxalicum is CCTCC No.M2019233. The phosphorus-dissolving penicillium oxalicum can grow well in a solid culture medium using calcium phosphate or iron phosphate as the only phosphorus source, the penicillium oxalicum canutilize insoluble phosphorus compounds as the growth substance after being cultured for 48 hours, and the penicillium oxalicum can grow a large number of colonies after being cultured for 3-7 days; ina liquid culture medium, the penicillium oxalicum can efficiently utilize calcium phosphate or iron phosphate with the maximum concentration being 50g / L to produce up to 2500mg / L of soluble phosphate. The penicillium oxalicum in a culture medium added with solid calcium phosphate can produce oxalic acid, formic acid, malic acid, acetic acid and lactic acid through metabolism, and the secretion quantity reaches up to 215.73mg / L. The microbial strain resource which is separated from a soil environment for the first time has a good application value in farmland soil phosphorus improving, soil fertility improving and soil heavy metal contamination comprehensive treatment promoting.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a phospholytic fungus Penicillium oxalicum PSF-4 with broad-spectrum acid-producing properties and its application. Background technique [0002] Phosphorus is one of the macroelements necessary for the growth and development of organisms, and is an important component of biofilms, nucleic acids, and many macromolecules (such as adenosine triphosphate). Phosphorus is the most important nutrient for plant growth after nitrogen. Phosphorus is transformed and flows between plants, animals, microorganisms and soil in the form of different compounds. Animals and microorganisms can supplement the required phosphorus elements through predation or catabolism, while plants mainly realize the biological activity in the soil through plant roots. Available phosphorus (mainly including primary phosphoric acid H 2 PO 4 - and secondary phosphate HPO 4 2- )Absorpt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/80
CPCB09C1/105C12N1/14C12N1/145C12R2001/80
Inventor 田江蒋逸凡葛飞李峰郝少芬张浩陈箫
Owner XIANGTAN UNIV
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